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2.4.1.264: D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase

This is an abbreviated version!
For detailed information about D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase, go to the full flat file.

Reaction

UDP-alpha-D-glucuronate
+
alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol
=
UDP
+
beta-D-GlcA-(1->2)-alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol

Synonyms

gumK

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.264 D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase

Engineering

Engineering on EC 2.4.1.264 - D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D157A
mutation in UDP binding site, loss of activity
D157E
mutation in UDP binding site, loss of activity
D157N
mutation in UDP binding site, loss of activity
D207A
mutation in UDP binding site, kcat/Km for UDP-glucuronate is identical to wild-type value
D234A
mutation in UDP binding site, kcat/Km for UDP-glucuronate is identical to wild-type value
E192A
mutation in UDP binding site, kcat/Km for UDP-glucuronate is identical to wild-type value
E272A
mutation in UDP binding site, kcat/KM is 4.6fold lower than wild-type value
E272D
mutation in UDP binding site, kcat/KM is 2fold lower than wild-type value
K307A
mutation in UDP binding site, kcat/KM is 560fold lower than wild-type value
M231A
mutation in UDP binding site, kcat/Km for UDP-glucuronate is similar to wild-type value
Q310A
mutation in UDP binding site, kcat/KM is 22.2fold lower than wild-type value
Y292A
mutation in UDP binding site, kcat/KM is 14.7fold lower than wild-type value