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2.4.1.255: protein O-GlcNAc transferase

This is an abbreviated version!
For detailed information about protein O-GlcNAc transferase, go to the full flat file.

Word Map on EC 2.4.1.255

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
[protein]-L-serine
=
UDP
+
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine

Synonyms

beta-N-acetylglucosaminidase, EGF domain-specific O-GlcNAc transferase, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, EOGT1, epidermal growth factor domain-specific O-GlcNAc transferase, glycosyltransferase OGT, Gtf1, GtfA, hOGT, HsOGT, human OGT, mammalian OGT, mOGT, More, N-acetylglucosamine transferase, N-acetylglucosamine-peptide N-acetylglucosaminyltransferase, N-acetylglucosaminyltransferase, ncOGT, nuclear cytoplasmic OGT, nucleocytoplasmic glycosyltransferase, nucleocytoplasmic OGT, O-GlcNAc protein, O-GlcNAc transferase, O-GlcNAc transferase OGT, O-GlcNAc-transferase, O-GlcNAcase, O-glycosylated protein p135, O-linked beta-N-acetylglucosamine transferase, O-linked beta-N-acetylglucosaminyltransferase, O-linked GlcNAc transferase, O-linked N-acetylglucosamine transferase, O-linked N-acetylglucosaminyltransferase, O-N-acetylglucosamine (O-GlcNAc) transferase, OGT, OGT p110, OGT protein, ogta, OGTase, OGTase protein, ogtb, p135, Protein SECRET AGENT, SEC, SECRET AGENT, sOGT, spindly, SPY, super sex combs, Sxc, Synpcc7942_0051, transferase OGT, UDP-GlcNAc: polypeptide O-N-acetylglucosaminyltransferase, UDP-GlcNAc:polypeptidyl transferase, UDP-N-acetylglucosaminyl transferase, uridine diphosphate-N-acetyl-D-glucosamine:polypeptidyltransferase, uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylaminyltransferase, uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase, XcOGT, XcOGT protein

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.255 protein O-GlcNAc transferase

Substrates Products

Substrates Products on EC 2.4.1.255 - protein O-GlcNAc transferase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ac4-5S-GlcNAc + [protein]-L-serine
UDP + ?
show the reaction diagram
the donor substrate analogues Ac4-5S-GlcNAc and benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside, might reduce the flux through the hexosamine pathway and reduce the amount of intracellular UDP-GlcNAc with potential side effects on glycan synthesis
-
-
?
adhesin PsrP + UDP-GlcNAc
? + UDP
show the reaction diagram
benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside + [protein]-L-serine
UDP + ?
show the reaction diagram
the donor substrate analogues Ac4-5S-GlcNAc and benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside, might reduce the flux through the hexosamine pathway and reduce the amount of intracellular UDP-GlcNAc with potential side effects on glycan synthesis
-
-
?
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
show the reaction diagram
casein kinase II + UDP-GlcNAc
? + UDP
show the reaction diagram
-
-
-
-
?
casein kinase II + UDP-N-azidoacetylglucosamine
? + UDP
show the reaction diagram
-
-
-
-
?
casein kinase II peptide + UDP-GlcNAc
? + UDP
show the reaction diagram
-
-
-
-
?
casein kinase II peptide + UDP-GlcNAc
UDP + ?
show the reaction diagram
-
much poorer substrate than Nup 62
-
-
?
crystalline alpha + UDP-GlcNAc
? + UDP
show the reaction diagram
-
i.e. small heat-shock protein crystalline alpha
-
-
?
GSK-3beta + UDP-GlcNAc
UDP + ?
show the reaction diagram
-
rabbit skeletal muscle glycogen synthase kinase (GSK) -3beta
-
-
?
KENSPCVTPVSTA + UDP-GlcNAc
? + UDP
show the reaction diagram
-
-
-
?
KKKYPGGSTPVSSANMM + UDP-4-deoxy-GalNAc
? + UDP
show the reaction diagram
-
-
-
?
KKKYPGGSTPVSSANMM + UDP-4-deoxy-GlcNAc
? + UDP
show the reaction diagram
22.2% yield
-
-
?
KKKYPGGSTPVSSANMM + UDP-6-deoxy-GalNAc
? + UDP
show the reaction diagram
37.7% yield
-
-
?
KKKYPGGSTPVSSANMM + UDP-6-deoxy-GlcNAc
? + UDP
show the reaction diagram
85% yield
-
-
?
KKKYPGGSTPVSSANMM + UDP-GlcNAc
? + UDP
show the reaction diagram
peptide acceptor derived from casein kinase II
-
-
?
KKKYPGGSTPVSSANMM + UDP-GlcNAc
UDP + ?
show the reaction diagram
-
Pep-CKII, known natural substrate for OGT
-
-
?
KKKYPGGSTPVSSANMM + UDP-GlcNAz
? + UDP
show the reaction diagram
peptide acceptor derived from casein kinase II
-
-
?
KKKYPGGSTPVSSANMM + UDP-GlcNPr
? + UDP
show the reaction diagram
the close vicinity between Met501 and the N-acyl group of GlcNPr, as well as the hydrophobic environment near Met501, are responsible for the selective binding of UDP-GlcNPr
-
-
?
mitochondrial motor-adaptor protein milton + UDP-GlcNAc
? + UDP
show the reaction diagram
-
-
mitochondrial motor-adaptor protein Milton is a required substrate for OGT to arrest mitochondrial motility by mapping and mutating the key O-GlcNAcylated serine residues
-
?
nucleoporin p62 + UDP-GlcNAc
? + UDP
show the reaction diagram
-
high affinity substrate
-
-
?
nucleoporin p62 + UDP-N-azidoacetylglucosamine
? + UDP
show the reaction diagram
-
-
-
?
OIP106 protein + UDP-GlcNAc
O-GlcNAc-OIP106 protein + UDP
show the reaction diagram
-
N-terminal deletions of OIP106 are generated as S-tagged constructs: DELTAnCC, DELTA491, DELTA639, DELTA859
-
-
?
RBL-2 + UDP-GlcNAc
? + UDP
show the reaction diagram
acceptor RBL-2 is a key regulator of entry into cell division. Residue Ser420 is a possible O-GlcNAc site in RBL-2. Substitution of Ser 420 inhibits OGT activity
-
-
?
UDP-GlcNAc + Abi2 protein
UDP + N-acetly-D-glucosaminyl-[Abi2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Abl2 protein
UDP + N-acetly-D-glucosaminyl-[Ab12 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Ablim2 protein
UDP + N-acetly-D-glucosaminyl-[Ablim2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Add1 protein
UDP + N-acetly-D-glucosaminyl-[Add1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + AIPVSREEK
UDP + AIPV-(GlcNAc)SREEK
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Amot protein
UDP + N-acetly-D-glucosaminyl-[Amot protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Arhgap32 protein
UDP + N-acetly-D-glucosaminyl-[Arhgap32 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Atf2 protein
UDP + N-acetly-D-glucosaminyl-[Atf2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + beta-amyloid associated protein
GlcNAc-beta-amyloid associated protein + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + c-MYC intron binding protein 1
UDP + N-acetyl-D-gluosaminyl-[c-MYC intron binding protein 1]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + calcium/calmodulin-dependent kinase IV
UDP + N-acetyl-D-glucosaminyl-[calcium/calmodulin-dependent kinase IV]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + CARM1 protein
UDP + N-acetyl-D-glucosaminyl-[CARM1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + CKII peptide
UDP + N-acetyl-D-glucosaminyl-[CKII peptide]
show the reaction diagram
UDP-GlcNAc + CKII peptide
UDP + N-acetylglucosaminyl-CKII alpha-peptide
show the reaction diagram
PGGSTPVSSANMM
-
-
?
UDP-GlcNAc + Clasp2 protein
UDP + N-acetly-D-glucosaminyl-[Clasp2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Clip1 protein
UDP + N-acetly-D-glucosaminyl-[Clip1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Cnot4 protein
UDP + N-acetly-D-glucosaminyl-[Cnot4 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Cnskr2 protein
UDP + N-acetly-D-glucosaminyl-[Cnskr2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Corp1b protein
UDP + N-acetly-D-glucosaminyl-[Corp1b protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Crtc1 protein
UDP + N-acetly-D-glucosaminyl-[Crtc1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + CSNK1D
CSNK1D-GlcNAc + UDP
show the reaction diagram
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + DCTN1
DCTN1-GlcNAc + UDP
show the reaction diagram
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + DELTA639 protein
UDP + N-acetyl-D-glucosaminyl-[DELTA639 protein]
show the reaction diagram
-
N-terminal truncation of OIP106, able to bind and pull down OGT, indicates that the potential OGT-binding domain localized to within residues 639-859 in the C-terminus of OIP106
-
-
?
UDP-GlcNAc + DELTAnCC protein
UDP + N-acetyl-D-glucosaminyl-[DELTAnCC protein]
show the reaction diagram
-
N-terminal truncation of OIP106, able to bind and pull down OGT, indicates that the potential OGT-binding domain localized to within residues 639-859 in the C-terminus of OIP106
-
-
?
UDP-GlcNAc + Dlgap2 protein
UDP + N-acetly-D-glucosaminyl-[Dlgap2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Dvl1 protein
UDP + N-acetly-D-glucosaminyl-[Dv11 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + dynamin-related protein 1
UDP + dynamin-related protein 1-GlcNAc
show the reaction diagram
dynamin-related protein 1 is O-linked-N-acetyl-glucosamine-glycosylated at threonine 585 and 586
-
-
?
UDP-GlcNAc + Enah protein
UDP + N-acetly-D-glucosaminyl-[Enah protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Foxk2 protein
UDP + N-acetly-D-glucosaminyl-[Foxk2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Foxp1 protein
UDP + N-acetly-D-glucosaminyl-[Foxp1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Frs3 protein
UDP + N-acetly-D-glucosaminyl-[Frs3 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + glutathione S-transferase
glutathione S-transferase-GlcNAc + UDP
show the reaction diagram
UDP-GlcNAc + glutathione S-transferase-CARM1
glutathione S-transferase-CARM1-GlcNAc + UDP
show the reaction diagram
UDP-GlcNAc + glutathione S-transferase-MYPT1
glutathione S-transferase-MYPT1-GlcNAc + UDP
show the reaction diagram
UDP-GlcNAc + H2O
UDP + GlcNAc
show the reaction diagram
UDP-GlcNAc + host cell factor C1
UDP + N-acetyl-D-gluosaminyl-[host cell factor C1]
show the reaction diagram
the enzyme both O-GlcNAcylates the HCF-1N subunit and directly cleaves the host cell factor-1PRO repeat
-
-
?
UDP-GlcNAc + ITISETPSSTTTTQITK
UDP + ITI-(GlcNAc)SETPSSTTTTQITK
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + KKFELLPTPPLSPSRR
UDP + KKFELLP-(GlcNAc)TPPLSPSRR
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Mamld1 protein
UDP + N-acetly-D-glucosaminyl-[Mamld1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + MYPT1
MYPT1-GlcNAc + UDP
show the reaction diagram
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + Nav1 protein
UDP + N-acetly-D-glucosaminyl-[Nav1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Ncoa1 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Ncoa2 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Ncoa5 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa5 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + NFATc1
O-GlcNAc-NFATc1 + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Nfia protein
UDP + N-acetly-D-glucosaminyl-[Nfia protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Notch epidermal growth factor 20 repeat
UDP + N-acetyl-D-gluosaminyl-[Notch epidermal growth factor 20 repeat]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Nr3c1 protein
UDP + N-acetly-D-glucosaminyl-[Nr3c1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Nup 62 protein
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Nup62 protein
UDP + N-acetyl-D-glucosaminyl-[Nup62 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Nup62 protein
UDP + N-acetyl-D-glucosmainyl-[Nup62 protein]
show the reaction diagram
UDP-GlcNAc + O-GlcNAcase
O-GlcNAcase-GlcNAc + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + p62 protein
UDP + N-acetyl-D-glucosaminyl-[p62 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + PGC-1alpha
UDP + N-acetyl-D-gluosaminyl-[PGC-1alpha]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + PGGSTPVS(PO3)-SANMM
? + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + PGGSTPVSSANMM
UDP + PGGSTPV-(GlcNAc)SSANMM
show the reaction diagram
-
PGGSTPVSSANMM is the best acceptor
-
-
?
UDP-GlcNAc + Plec protein
UDP + N-acetly-D-glucosaminyl-[Plec protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Psd3 protein
UDP + N-acetly-D-glucosaminyl-[Psd3 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Rapgef2 protein
UDP + N-acetly-D-glucosaminyl-[Rapgef2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Rasgrf2 protein
UDP + N-acetly-D-glucosaminyl-[Rasgrf2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + SAP130
SAP130-GlcNAc + UDP
show the reaction diagram
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + Sirt2 protein
UDP + N-acetly-D-glucosaminyl-[Sirt2 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Ss18l1 protein
UDP + N-acetly-D-glucosaminyl-[Ss1811 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Stat3 protein
UDP + N-acetly-D-glucosaminyl-[Stat3 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Tab1 protein
UDP + N-acetly-D-glucosaminyl-[Tab1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + TAB1 protein
UDP + N-acetyl-D-glucosaminyl-[TAB1 protein]
show the reaction diagram
UDP-GlcNAc + tau protein
GlcNAc-tau protein + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Tbr1 protein
UDP + N-acetly-D-glucosaminyl-[Tbr1 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Tle4 protein
UDP + N-acetly-D-glucosaminyl-[Tle4 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Tnik protein
UDP + N-acetly-D-glucosaminyl-[Tnik protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + TRAK1 protein
UDP + N-acetyl-D-glucosaminyl-[TRAK1 protein]
show the reaction diagram
UDP-GlcNAc + transcription factor FoxM1
O-GlcNAc-transcription factor FoxM1 + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + transcription factor NFAT
UDP + N-acetyl-D-glucosaminyl-[transcription factor NFAT]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + transcription factor NFkappaB
UDP + N-acetyl-D-glucosaminyl-[transcription factor NFkappaB]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + YPGGSTPVSSANMM
UDP + YPGGSTPVS-3-O-(N-acetyl-D-glucosaminyl)-SANMM
show the reaction diagram
-
-
-
?
UDP-GlcNAc + YSDSPSTST
UDP + ?
show the reaction diagram
UDP-GlcNAc + YSDSPSTST
UDP + GlcNAc-YSDSPSTST
show the reaction diagram
-
-
-
?
UDP-GlcNAc + YSDSPSTST
YSDSP-(GlcNAc)STST + UDP
show the reaction diagram
-
coupled enzyme assay of C-654
-
-
?
UDP-GlcNAc + YSPTSPSYSPTSPS
UDP + Y-(GlcNAc)SPT-(GlcNAc)SPSYSPT-(GlcNAc)SPS
show the reaction diagram
-
YSPTSPSYSPTSPS is a poor substrate
-
-
?
UDP-GlcNAc + Znf532 protein
UDP + N-acetly-D-glucosaminyl-[Znf532 protein]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + [YSPTSPSYSPTSPS]5
UDP + [Y-(GlcNAc)SP-(GlcNAc)TSPSYSP-(GlcNAc)TSPS]5
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc DELTA491 protein
UDP + N-acetyl-D-glucosaminyl-[DELTA491 protein]
show the reaction diagram
-
N-terminal truncation of OIP106, able to bind and pull down OGT, indicates that the potential OGT-binding domain localized to within residues 639-859 in the C-terminus of OIP106
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + FITC-YAVVPVSK peptide
UDP + ?
show the reaction diagram
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [Nup62 protein]-L-serine
UDP + [Nup62 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [octamer-binding protein 4]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + [octamer-binding protein 4]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
show the reaction diagram
UDP-N-acetyl-D-glucosamine + KKKYPGGSTPVSSANMM
UDP + ?
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + YPGGSTPVSSANMM
UDP + YPGGSTPVS-3-O-(N-acetyl-D-glucosaminyl)-SANMM
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + [protein]-L-serine
?
show the reaction diagram
UDP-N-acetyl-5-deoxy-5-thio-alpha-D-glucosamine is a very poor (3200times slower) donor substrate compared to UDP-N-acetyl-D-glucosamine
-
-
?
UDP-N-acetyl-D-glucosamine + [protein]-L-serine
UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine
show the reaction diagram
the enzyme transfers N-acetylglucosamine from the sugar donor UDP-GlcNAc onto specific serine or threonine residues of nucleocytoplasmic proteins with inversion of configuration at the anomeric center
-
-
?
UDP-N-acetyl-D-glucosamine + [protein]-L-serine
UDP + [protein]-O3-(N-acetyl-D-glucosaminyl)-L-serine
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + [protein]-L-threonine
UDP + [protein]-O3-(N-acetyl-D-glucosaminyl)-L-threonine
show the reaction diagram
-
-
-
-
?
YSDSPSTST + UDP-GlcNAc
UDP + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-