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2.4.1.255: protein O-GlcNAc transferase

This is an abbreviated version!
For detailed information about protein O-GlcNAc transferase, go to the full flat file.

Word Map on EC 2.4.1.255

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
[protein]-L-serine
=
UDP
 +
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine

Synonyms

beta-N-acetylglucosaminidase, EGF domain-specific O-GlcNAc transferase, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, EOGT1, epidermal growth factor domain-specific O-GlcNAc transferase, glycosyltransferase OGT, Gtf1, GtfA, hOGT, HsOGT, human OGT, mammalian OGT, mOGT, More, N-acetylglucosamine transferase, N-acetylglucosamine-peptide N-acetylglucosaminyltransferase, N-acetylglucosaminyltransferase, ncOGT, nuclear cytoplasmic OGT, nucleocytoplasmic glycosyltransferase, nucleocytoplasmic OGT, O-GlcNAc protein, O-GlcNAc transferase, O-GlcNAc transferase OGT, O-GlcNAc-transferase, O-GlcNAcase, O-glycosylated protein p135, O-linked beta-N-acetylglucosamine transferase, O-linked beta-N-acetylglucosaminyltransferase, O-linked GlcNAc transferase, O-linked N-acetylglucosamine transferase, O-linked N-acetylglucosaminyltransferase, O-N-acetylglucosamine (O-GlcNAc) transferase, OGT, OGT p110, OGT protein, ogta, OGTase, OGTase protein, ogtb, p135, Protein SECRET AGENT, SEC, SECRET AGENT, sOGT, spindly, SPY, super sex combs, Sxc, Synpcc7942_0051, transferase OGT, UDP-GlcNAc: polypeptide O-N-acetylglucosaminyltransferase, UDP-GlcNAc:polypeptidyl transferase, UDP-N-acetylglucosaminyl transferase, uridine diphosphate-N-acetyl-D-glucosamine:polypeptidyltransferase, uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylaminyltransferase, uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase, XcOGT, XcOGT protein

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.255 protein O-GlcNAc transferase

Cloned

Cloned on EC 2.4.1.255 - protein O-GlcNAc transferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of more than 50 of human cDNA clones and other ESTs suggest that the mammalian ogt gene encodes several splice variants
-
baculovirus expression
-
cloning and expression of OGT in insect cells
-
expressed in Escherichia coli
expressed in Escherichia coli and expressed in HeLa cells
expressed in Escherichia coli and HEK-293 cells
expressed in Escherichia coli Tuner cells
expressed in HEK-293T cells
expressed in Saccharomyces cerevisiae
-
expression in Escherichia coli
expression in Escherichia coli, N-terminally truncated construct starting at amino acid 353 in tetratricopeptide repeat TPR 10 (D1–352)
expression in HeLa cells
-
full length and functional human mOGT is difficult to be expressed in Escherichia coli, truncated mOGT recombinant (C-654) is constructed and expressed
-
modified with both O-GlcNAc and tyrosine phosphorylation when overexpressed in insect cells
-
ncOGT, mOGT, and sOGT constructs are cloned into a modified pET-24b vector for expression as C-terminal His8 fusions. Expression in Escherichia coli
-
overexpressed FLAGtagged human OGT in VSM cells
-
overexpression of the Ogt gene encoding the 110 kDa alpha-subunit in Escherichia coli and transiently in HEK293 cells, DNA sequence analysis: gene Ogt is no member of a multigene family, the sequence contains multiple tandem repeats of the tetratricopeptide repeat motif
purified recombinant 110 kDa subunit of OGTase expressed in Escherichia coli
-
recombinant expression in Escherichia coli
-
recombinant production of human sOGT and ncOGT in Escherichia coli
-
SEC protein expressed in Escherichia coli
the mitochondrial version of human OGT is subcloned into the pSos vector in-frame with the human Sos protein
-
using the insect cell baculovirus system to overexpress p110 subunit
-
when co-expressed in Escherichia coli SEC modifies PPV-CP