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2.4.1.252: GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase

This is an abbreviated version!
For detailed information about GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase, go to the full flat file.

Reaction

GDP-mannose
+
D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
=
GDP
+
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol

Synonyms

AceA, alpha-mannosyltransferase AceA, GDP-mannosyltransferase, gumH

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.252 GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase

Cloned

Cloned on EC 2.4.1.252 - GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in fusion with maltose-binding protein in Escherichia coli BL21(DE3) cells and as His-tagged protein in Rosetta(DE3)pLysS cells
-
overproduced in Escherichia coli. The aceA ORF is subcloned into the expression vector pET29 in frame with the S-tag epitope. The recombinant protein is identified, and culture conditions are optimised for production of the soluble protein
-
recombinant mutant AceA expressed in Escherichia coli and in Xanthomonas campestris gumH- strain
-
recombinantly expressed in Escherichia coli
-