2.4.1.252: GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase
This is an abbreviated version!
For detailed information about GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase, go to the full flat file.
Reaction
Synonyms
AceA, alpha-mannosyltransferase AceA, GDP-mannosyltransferase, gumH
ECTree
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Application
Application on EC 2.4.1.252 - GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase
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analysis
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method for real-time detection of recombinant GumH enzymatic activity using a Quartz Crystal Microbalance with dissipation monitoring, QCM-D. Monitoring the real-time cellobiose-diphosphate-polyprenol carrier lipid-QCM-D transducer in the presence of GDP-man and GumH enzyme shows a mass increase, indicating the transfer of mannose. The determination of mannosyltransferase function is validated by a HPLC method developed for determination of GDP produced by enzymatic reaction
synthesis
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the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide