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2.4.1.25: 4-alpha-glucanotransferase

This is an abbreviated version!
For detailed information about 4-alpha-glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.25

Reaction

maltononaose
+
maltotriose
=
maltoundecaose
 +
D-glucose

Synonyms

(1->4)-alpha-D-glucan:(1->4)-alpha-D-glucan 4-alpha-D-glycosyltransferase, 4-alpha-D-alpha-glucanotransferase, 4-alpha-glucanotransferase, 4-alpha-GTase, 4alphaGT, 4alphaGTase, AgtA, AgtB, alpha-1,4-glucanotransferase, alpha-1,4-transferase, alpha-amylase-like transglycosylase, alpha-glucanotransferase, alphaGT, alphaGTase, AMase, amylmaltase, amylomaltase, At2g40840, CGTase, Cqm1, D-enzyme, debranching enzyme, debranching enzyme maltodextrin glycosyltransferase, dextrin glycosyltransferase, dextrin glycosyltransferase,, dextrin transglycosylase, disproportionating enzyme, DPE1, DPE2, EC 2.4.1.3, exo-MGTase, GDE, GH77, GH77 amylomaltase, glycogen debranching enzyme, GTase, MalQ, maltodextrin glycosyltransferase, maltosyltransferase, MgtA, MmtA, More, MQ-01, MTase, oligo-1,4-1,4-glucantransferase, PyAMase, TAalphaGT, TAalphaGTase, TLGT, TmalphaGT, TreX, TS alpha GTase, TSalphaGT

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.25 4-alpha-glucanotransferase

Temperature Stability

Temperature Stability on EC 2.4.1.25 - 4-alpha-glucanotransferase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 40
-
at short incubation time for 15 min at 40°C, the remaining activity of the wild type enzyme is 39.5%, whereas for 30 min incubation, the activity remained is 15.2%. At 35°C for a longer incubation time of 3 h, the remaining activity of the wild type enzyme is 45%
35 - 45
-
the specific activity remaining after 10 min at 35°C for the wild type enzyme is 49.3% while that for mutant enzyme N287Y is 91.4%. At 40°C, no activity of the wild type enzyme is left after 10 min but mutant N287Y shows 34.3% remaining activity. At 45°C, the wild type loses all activity after 10 min incubation time while 28.6% of the activity of mutant N287Y still remains
37 - 42
-
D-enzyme, activity decreases rapidly above 37°C and is almost completely lost at 42°C
37 - 80
-
stable at 37°C, retains more than 90% of its maximum activity between 55-80°C, half-life of activity of about 3 h at 80°C
4
-
quite stable at low temperatures
45
-
stable up to
50 - 80
-
the recombinant enzyme maintains more than 80% of its activity at a temperature range of 50-80°C and a pH range of 5.0-9.0
51.5
melting temperature
60
-
loses activity when incubated for 10 min
60 - 70
-
the thermostability at 60-70°C in pH 6.0 potassium phosphate buffer, after 2 h of incubation time, shows approximately 70-80% remaining activity
87
-
half-life: 506 min
89
-
half-life: 114 min