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2.4.1.245: alpha,alpha-trehalose synthase

This is an abbreviated version!
For detailed information about alpha,alpha-trehalose synthase, go to the full flat file.

Reaction

NDP-alpha-D-glucose
+
D-glucose
=
alpha,alpha-trehalose
+
NDP

Synonyms

PH1035, TaTS, TmTreT, trehalose glycosyltransferring synthase, Trehalose synthase, TreT

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.245 alpha,alpha-trehalose synthase

Engineering

Engineering on EC 2.4.1.245 - alpha,alpha-trehalose synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D134E
the mutant shows 10% reduced activity in the forward reaction, and 50% reduced activity in the reverse reaction
D134R
inactive mutant
D274R/D275A
inactive mutant
E326A
inactive mutant
F85R
the mutant shows 95% reduced activity in the forward reaction, and no activity in the reverse reaction
F85Y
the mutant shows 10% reduced activity in the forward reaction, and 40% reduced activity in the reverse reaction
H155D
inactive in the forward reaction, 80% reduced activity in the reverse reaction compared to wild-type
H92A
the mutant shows wild-type activity in the forward reaction, and 90% reduced activity in the reverse reaction
K209R
the mutant shows wild-type activity
Q96A
the mutant shows 10% reduced activity in the forward reaction, and 90% reduced activity in the reverse reaction
R239A
inactive in the reverse reaction, 10% reduced activity in the forward reaction compared to wild-type
T49H
the mutant shows 90% reduced activity in the forward reaction, and 98% reduced activity in the reverse reaction
T49R
inactive mutant
V309L
the mutant shows wild-type activity in the forward reaction, and 25% reduced activity in the reverse reaction