2.4.1.243: 6G-fructosyltransferase
This is an abbreviated version!
For detailed information about 6G-fructosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.243
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2.4.1.243
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fructans
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onion
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fructosyltransferases
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invertases
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1-kestose
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sucrose:sucrose
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fructose
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vacuolar
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lolium
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perenne
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perennial
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inulin
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ryegrass
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pichia
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6-fructosyltransferase
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polymerization
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fructooligosaccharides
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grass
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sucrose:fructan
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sheaths
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sucrose-binding
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exohydrolase
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officinalis
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asparagus
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blades
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cool-season
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agave
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sink-source
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forage
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tequilana
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agriculture
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neokestose
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beta-fructosidase
- 2.4.1.243
- fructans
- onion
- fructosyltransferases
- invertases
- 1-kestose
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sucrose:sucrose
- fructose
- vacuolar
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lolium
- perenne
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perennial
- inulin
- ryegrass
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pichia
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6-fructosyltransferase
- polymerization
- fructooligosaccharides
- grass
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sucrose:fructan
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sheaths
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sucrose-binding
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exohydrolase
- officinalis
- asparagus
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blades
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cool-season
- agave
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sink-source
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forage
- tequilana
- agriculture
- neokestose
- beta-fructosidase
Reaction
Synonyms
6(G)-fructosyltransferase/2,1-fructan:2,1-fructan 1-fructosyltransferase, 6G-FFT, 6G-FFT/1-FFT, 6G-FFT1, 6G-FFT2, AoFT1, Atq6G-FFT-1, Atq6G-FFT-2, fructan-fructan 6G-fructosyltransferase, fructan: fructan 6G-fructosyltransferase, fructan:fructan 6G-fructosyltransferase, fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase
ECTree
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Substrates Products
Substrates Products on EC 2.4.1.243 - 6G-fructosyltransferase
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REACTION DIAGRAM
1-kestose + 1-kestose
D-sucrose + 1G-kestotetraose + 6G-kestotetraose
6G-FFT activity
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-
?
1-kestose + sucrose
1,1-kestotetraose + 1G-kestotetraose + 6G-kestotetraose
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prolonged incubation produces a complex fructan series with a higher degree of polymerization
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?
1F(1-beta-D-fructofuranosyl)2 sucrose + sucrose
1F,6G-di-beta-D-fructofuranosylsucrose + ?
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i.e. nystose
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?
1F(1-beta-D-fructofuranosyl)3 sucrose + sucrose
1F,6G-di-beta-D-fructofuranosylsucrose + ?
1F(1-beta-D-fructofuranosyl)sucrose + sucrose
1F,6G-di-beta-D-fructofuranosylsucrose + ?
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i.e. 1-kestose
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?
1F,6G-di-beta-D-fructofuranosylsucrose + sucrose
1-kestose + 1-kestose
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reverse reaction
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r
4 1-kestose
sucrose + 1G-kestotetraose + 6G-kestotetraose + H2O
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?
3 neokestose + H2O
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plus a 4c tetrasaccharide moiety
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?
1-kestose + 3 sucrose
3 neokestose + H2O
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plus a 4c tetrasaccharide moiety
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?
1-kestose + 3 sucrose
3 neokestose + H2O
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plus fructans of the inulin neoseries with a degree of polymerization up to six
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?
1,1-kestotetraose + 1,6-kestotetraose
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neither this fructan nor the subsequent mixed series is found in vivo in the tissues of Lolium perenne
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?
1-kestose + sucrose
1,1-kestotetraose + 1,6-kestotetraose
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neither this fructan nor the subsequent mixed series is found in vivo in the tissues of Lolium perenne
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?
1F,6G-di-beta-D-fructofuranosylsucrose + ?
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i.e. fructosyl nystose
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?
1F(1-beta-D-fructofuranosyl)3 sucrose + sucrose
1F,6G-di-beta-D-fructofuranosylsucrose + ?
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?
?
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the Atq6GFFT-1 consensus sequence is DPCG, Atq6GFFT-1 and Atq6G-1FFT-2 utilize primarily 1-kestose as a substrate, a Asn/Trp couple is involved in substrate recognition
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?
additional information
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the Atq6GFFT-1 consensus sequence is DPCG, Atq6GFFT-1 and Atq6G-1FFT-2 utilize primarily 1-kestose as a substrate, a Asn/Trp couple is involved in substrate recognition
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?
additional information
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plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides
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?
additional information
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main products are inulin neoseries fructan, such as 1F,6G-di-beta-D-fructofuranosylsucrose and neokestose
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?
additional information
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the enzyme possesses both fructan:fructan 6G-fructosyltransferase and fructan:fructan 1-fructosyltransferase activities
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?
additional information
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the enzyme possesses both fructan:fructan 6G-fructosyltransferase and fructan:fructan 1-fructosyltransferase activities
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?
additional information
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Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose
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?
additional information
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Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose
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?
additional information
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both 1-kestotriose and 6G-kestotriose acts as fructosyl acceptors, producing 1- and 6-kestotetraose (bifurcose) and 6G,6-kestotetraose, respectively. 1-Kestotriose is not a fructosyl donor since incubation with 1-kestotriose alone does not lead to the synthesis of a 6-linked fructan. Bifurcose formation ceases and 6G,6-kestotetraose is formed instead, when recombinant fructan:fructan 6G-fructosyltransferase, 6G-FFT, is introduced in the enzyme assay with sucrose and 1-kestotriose as substrates
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?
additional information
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both 1-kestotriose and 6G-kestotriose acts as fructosyl acceptors, producing 1- and 6-kestotetraose (bifurcose) and 6G,6-kestotetraose, respectively. 1-Kestotriose is not a fructosyl donor since incubation with 1-kestotriose alone does not lead to the synthesis of a 6-linked fructan. Bifurcose formation ceases and 6G,6-kestotetraose is formed instead, when recombinant fructan:fructan 6G-fructosyltransferase, 6G-FFT, is introduced in the enzyme assay with sucrose and 1-kestotriose as substrates
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?
additional information
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Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose
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?
additional information
?
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both 1-kestotriose and 6G-kestotriose acts as fructosyl acceptors, producing 1- and 6-kestotetraose (bifurcose) and 6G,6-kestotetraose, respectively. 1-Kestotriose is not a fructosyl donor since incubation with 1-kestotriose alone does not lead to the synthesis of a 6-linked fructan. Bifurcose formation ceases and 6G,6-kestotetraose is formed instead, when recombinant fructan:fructan 6G-fructosyltransferase, 6G-FFT, is introduced in the enzyme assay with sucrose and 1-kestotriose as substrates
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?
additional information
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6G-kestotriose is formed by enzyme 6G-FFT, transferring fructose from 1-kestotriose to sucrose, while the 6G-kestotriose levels formed in the assays with added 1-kestotriose and sucrose are rather small. Rapid chain elongation of 6G-kestotriose into 1+6G-kestotetraose and possibly other higher degree of polymerization fructans
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?