2.4.1.243: 6G-fructosyltransferase

This is an abbreviated version!
For detailed information about 6G-fructosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.243

2.4.1.243

fructans

onion

fructosyltransferases

invertases

1-kestose

sucrose:sucrose

fructose

vacuolar

lolium

perenne

perennial

inulin

ryegrass

pichia

6-fructosyltransferase

polymerization

fructooligosaccharides

grass

sucrose:fructan

sheaths

sucrose-binding

exohydrolase

officinalis

asparagus

blades

cool-season

agave

sink-source

forage

tequilana

agriculture

neokestose

beta-fructosidase

Reaction

[1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside

[1-beta-D-fructofuranosyl-(2->1)-]n alpha-D-glucopyranoside

[1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside

[1-beta-D-fructofuranosyl-(2->1)-]n beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside

Synonyms

6(G)-fructosyltransferase/2,1-fructan:2,1-fructan 1-fructosyltransferase, 6G-FFT, 6G-FFT/1-FFT, 6G-FFT1, 6G-FFT2, AoFT1, Atq6G-FFT-1, Atq6G-FFT-2, fructan-fructan 6G-fructosyltransferase, fructan: fructan 6G-fructosyltransferase, fructan:fructan 6G-fructosyltransferase, fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.243 6G-fructosyltransferase

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Results	in table
5	Activating Compound
13	AA Sequence
1	Application
1	CAS Registry Number
6	Cloned(Commentary)
1	Expression
6	General Information
1	General Stability
9	Inhibitors
2	KM Value [mM]
1	Localization
7	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
28	Organism
4	pH Optimum
2	pH Range
3	pH Stability
3	pI Value
3	Posttranslational Modification
12	Protein Variants
4	Purification (Commentary)
1	Reaction
26	Reference
18	Source Tissue
2	Specific Activity [micromol/min/mg]
42	Substrates and Products (Substrate)
8	Subunits
35	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]

Engineering

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Engineering on EC 2.4.1.243 - 6G-fructosyltransferase

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N84A

Allium cepa

P92916

preferred addition of fructose to a terminal fructose instead of terminal glucose, producing inulin-type fructans

666611

N84A/S87N

Allium cepa

P92916

prefers synthesis of beta(2-1) linkages

N84G

no enzymic activity

N84Q

preferred addition of fructose to a terminal fructose instead of terminal glucose, producing inulin-type fructans

666611

N84S

Allium cepa

P92916

preferred addition of fructose to a terminal fructose instead of terminal glucose, producing inulin-type fructans

N84Y

no enzymic activity

S87D

no change in substrate or product specificity

S87N

N340D/W343R

the mutant shows 6G-FFT and 1-FFT activities that hold the middle between those of the N340D and W343R single mutants

706297

S415N

Lolium perenne

Q8S337

the mutant shows slightly lower 6G-FFT/1-FFT activities, but the ratio between the main reaction products is not significantly affected

706297

additional information

2 entries