2.4.1.237: flavonol 7-O-beta-glucosyltransferase

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For detailed information about flavonol 7-O-beta-glucosyltransferase, go to the full flat file.

Reaction

Synonyms

F 7 GT, F7GT, flavonol 7-O-glucosyltransferase, GT2, NTGT2, Q7OGT, quercetin 7OGT, quercetin-7-O-GT, UDP-glucose:flavonoid 7-O-glucosyltransferase, UDP-glucose:flavonol 7-O-glucosyltransferase, UDPG:flavonol 7-O-glucosyltransferase, UGT71F1, UGT73A4

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.237 flavonol 7-O-beta-glucosyltransferase

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Results	in table
5	Activating Compound
31	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
40	Inhibitors
20	KM Value [mM]
7	Metals/Ions
4	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
8	Organism
5	Pathway
14	pH Optimum
4	pI Value
5	Purification (Commentary)
2	Reaction
1	Reaction Type
8	Reference
16	Source Tissue
5	Specific Activity [micromol/min/mg]
4	Storage Stability
87	Substrates and Products (Substrate)
3	Subunits
17	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]

Storage Stability

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Storage Stability on EC 2.4.1.237 - flavonol 7-O-beta-glucosyltransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 15% loss of activity, 8 days: 60% loss of activity

Paederia scandens

-

489572

-20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 18% loss of activity, 8 days: 64% loss of activity

Euonymus alatus

-

489566

UGT71F1 can not be frozen nor stored at 4°C as 50% activity is lost within 24 h

Beta vulgaris

-

676370

UGT73A4 can be stored at 4°C or frozen at -20°C or -80°C for several months without requiring addition of stabilizing agents such as bovine serum albumin or glycerol

Beta vulgaris

-

676370