2.4.1.231: alpha,alpha-trehalose phosphorylase (configuration-retaining)

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For detailed information about alpha,alpha-trehalose phosphorylase (configuration-retaining), go to the full flat file.

Reaction

Synonyms

PoTPase, ScTPase, TP2, TP3, trehalose phosphorylase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.231 alpha,alpha-trehalose phosphorylase (configuration-retaining)

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Results	in table
4	Activating Compound
9	AA Sequence
1	Application
1	CAS Registry Number
2	Cloned(Commentary)
4	General Information
2	General Stability
40	Inhibitors
2	kcat/KM [mM/s]
21	Ki Value [mM]
44	KM Value [mM]
1	Metals/Ions
12	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
27	Organism
1	Pathway
15	pH Optimum
3	pH Range
2	pH Stability
2	pI Value
10	Protein Variants
10	Purification (Commentary)
7	Reaction
2	Reaction Type
25	Reference
24	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
57	Substrates and Products (Substrate)
7	Subunits
14	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
9	Temperature Stability [°C]
32	Turnover Number [1/s]

General Stability

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General Stability on EC 2.4.1.231 - alpha,alpha-trehalose phosphorylase (configuration-retaining)

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highly unstable during purification, glycerol, phosphate, dithiothreitol and PMSF stabilize

Agaricus bisporus

644907

unstable protein, half-life of no more than approximately 1.3 h in 20mM MES buffer, 25°C, pH 7.0. The enzyme can be stabilized on addition of alpha,alpha-trehalose (300 mM) which yields a half-life of 11.5 h. In the presence of 20% (w/v) glycerol, and 9 and 26% (w/v) PEG-4000, half-life is 6.5 h, 16 h and 70 h, respectively. Large improvements in stability by covalent protein modification with activated derivatives of PEG-5000

Pleurotus ostreatus

674984