2.4.1.230: kojibiose phosphorylase

This is an abbreviated version!
For detailed information about kojibiose phosphorylase, go to the full flat file.

CsKP, kojibiose phosphorylase, KPase, PsKP, Py04_1502, YcjT

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Results	in table
603	AA Sequence
3	Application
1	CAS Registry Number
7	Cloned(Commentary)
1	Crystallization (Commentary)
14	General Information
4	Inhibitors
6	kcat/KM [mM/s]
19	KM Value [mM]
4	Metals/Ions
4	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
26	Organism
1	Pathway
5	PDB ID
12	pH Optimum
1	pH Range
3	pH Stability
1	pI Value
33	Protein Variants
8	Purification (Commentary)
1	Reaction
3	Reaction Type
24	Reference
3	Specific Activity [micromol/min/mg]
62	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
1	Temperature Range [°C]
9	Temperature Stability [°C]
6	Turnover Number [1/s]

Purification on EC 2.4.1.230 - kojibiose phosphorylase

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chimeric enzymes

kojibiose phosphorylase is purified from a cell-free extract of Thermoanaerobacter brockii

recombinant enzyme

recombinant His-tagged LaMP loop 3 mutants from Escherichia coli strain BL21(DE3) by affinity chromatography and dialysis

recombinant wild-type and mutant enzymes