2.4.1.230: kojibiose phosphorylase
This is an abbreviated version!
For detailed information about kojibiose phosphorylase, go to the full flat file.
Word Map on EC 2.4.1.230
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2.4.1.230
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brockii
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thermoanaerobacter
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maltose
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phosphorylases
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phosphorolysis
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proteinase
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saccharide
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trehalose
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oligosaccharides
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keratinolytic
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1-phosphate
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corneum
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glycoside
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pepstatin
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albicans
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stratum
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trisaccharide
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nigerose
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synthesis
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saccharolyticus
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chymostatin
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2d-nmr
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l-sorbose
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dermatophytosis
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beta-d-glucose
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canis
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scalp
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caldicellulosiruptor
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hsqc-tocsy
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microsporum
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brevis
- 2.4.1.230
- brockii
-
thermoanaerobacter
- maltose
- phosphorylases
-
phosphorolysis
- proteinase
-
saccharide
- trehalose
- oligosaccharides
-
keratinolytic
- 1-phosphate
- corneum
- glycoside
- pepstatin
- albicans
-
stratum
- trisaccharide
- nigerose
- synthesis
- saccharolyticus
- chymostatin
-
2d-nmr
- l-sorbose
- dermatophytosis
- beta-d-glucose
- canis
-
scalp
-
caldicellulosiruptor
-
hsqc-tocsy
- microsporum
- brevis
Reaction
Synonyms
CsKP, kojibiose phosphorylase, KPase, PsKP, Py04_1502, YcjT
ECTree
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Crystallization
Crystallization on EC 2.4.1.230 - kojibiose phosphorylase
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purified enzyme in complex with glucose and phosphate and in complex with kojibiose and sulfate, Glc-PO4 crystals are obtained at 25°C by sitting drop vapor diffusion method, mixing 0.001 ml of 5.9 m/ml protein in 5 mM Tris/HCl, pH 7.5, with an equal volume of reservoir solution containing 5 mM D-glucose, 5 mM sodium phosphate, pH 8.5, 10% v/v 2-propanol, 10% w/v PEG 3350, and 0.1 M Tris/HCl, pH 8.5, the kojibiose-SO4 crystals are obtained at 25°C by hanging drop vapor diffusion method, mixing of 0.001 ml of 5.4 mg/ml protein solution in 5 mM Tris/HCl, pH 7.5, with an equal volume of reservoir solution containing 5 mM kojibiose, 0.2 M NaCl, 2.0 M (NH4)2SO4, and 0.1 M sodium cacodylate, pH 6.5, X-ray diffraction structure determination and analysis at 2.05-2.80 A resolution, structures PDB IDs 3WIR and 3WIQ, molecular replacement with the maltose phosphorylase structure from Lactobacillus brevis LbMP, PDB ID 1H54, as the search model