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2.4.1.217: mannosyl-3-phosphoglycerate synthase

This is an abbreviated version!
For detailed information about mannosyl-3-phosphoglycerate synthase, go to the full flat file.

Word Map on EC 2.4.1.217

Reaction

GDP-mannose
+
3-phospho-D-glycerate
=
GDP
+
2-(alpha-D-mannosyl)-3-phosphoglycerate

Synonyms

mannosyl-3-P-glycerate synthase, mannosyl-3-phosphoglycerate phosphatase, mannosyl-3-phosphoglycerate synthase, mannosyl-3-phosphoglycerate synthase/phosphatase, mannosylglycerate synthase, MGSD, MPG synthase, MPGP, MPGS, MPGS/GPGS, MpgS/P, synthase, mannosyl-3-phosphoglycerate

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.217 mannosyl-3-phosphoglycerate synthase

Purification

Purification on EC 2.4.1.217 - mannosyl-3-phosphoglycerate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native isozymes in a multistep procedure, recombinant isozymes from Escherichia coli by 3 chromatographic steps of cation exchange chromatography and gel filtration
native mannosyl-3-phosphoglycerate synthase is purified from Rubrobacter xylanophilus cells using a Q-Sepharose FF and a Mono-Q column, recombinant MpgS is purified by immobilized metal-affinity chromatography on a Ni-Sepharose column
purified from cell extracts
Q-Sepharose column chromatography, Resource Q column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration
-
recombinant enzyme and enzyme domains from Escherichia coli
-
recombinant enzyme from Escherichia coli strain BL21