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2.4.1.212: hyaluronan synthase

This is an abbreviated version!
For detailed information about hyaluronan synthase, go to the full flat file.

Word Map on EC 2.4.1.212

Reaction

UDP-alpha-D-glucuronate
+
N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
=
UDP
 +
beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]

Synonyms

CHAS2, CHAS3, class I hyaluronan synthase, CPS1, DG42 protein, HA synthase, HA synthase 3, HA1, HA2, HA3, HAS, HAS-1, HAS-2, HAS-3, HAS1, Has2, Has3, hasA, HASs, HsHAS1, HuHAS1, HyaD, hyaluronan synthase, hyaluronan synthase 1, hyaluronan synthase 2, hyaluronan synthase 3, hyaluronan synthase-1, hyaluronan synthase-2, hyaluronan synthases 2, hyaluronan synthethase, hyaluronate synthase, hyaluronate synthetase, hyaluronic acid synthase, hyaluronic acid synthetase, More, PmHAS, seHAS, XHAS1, XHAS2, XHAS3

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.212 hyaluronan synthase

Cloned

Cloned on EC 2.4.1.212 - hyaluronan synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a plasmid encoding FLAG epitope-tagged HAS3 for transfection of human prostate tumor cells is constructed, Tet-inducible 22Rv1 cells are generated
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DNA and amino acid sequence determination and analysis, chromosome mapping of isozymes HAS1-3, genetic organization
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ectopic expression of Flag- and 6myc-HAS2 in COS-1 cells as homodimers, co-expression with Flag-HAS3 leads to formation of heterodimers
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expression as a soluble active protein comprising residues 1-703
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expression in Bacillus subtilis
expression in COS-1 cells and rat 3Y1 fibroblasts
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expression in COS-7 cells
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Saccharomyces cerevisiae and Escherichia coli
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expression in yeast
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expression of both hyaluronan synthase and UDP-glucose-6-dehydrogenase in Lactococcus lactis
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expression of C-terminally His6-tagged Se-HAS in Escherichia coli strain C43
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expression of His-tagged enzyme in Escherichia coli
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expression of His-tagged wild-type and mutant enzymes in Escherichia coli
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expression of His-taggged residues 1-703 in Escherichia coli
expression of isozyme HAS2-MBP-fusion protein in Escherichia coli strain JM109
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expression of mutant enzymes in Escherichia coli. SeHAS(E327Q) and seHAS(E327K) are expressed at low levels, whereas seHAS(E327D) and the Lys48 mutants are expressed well
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expression of wild-type and mutant enzymes as His-tagged proteins in Escherichia coli
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expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain BJ5461
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expression of wild-type and mutants enzymes in Escherichia coli
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expression of wild-type FLAG-tagged human HAS1, HAS2, or HAS3, and of HAS T110A mutant enzyme in COS-7 cells
functional expression of isozymes HAS2 and HAS3 in rat epidermal keratinocytes as N-terminally GFP-tagged protein, recombinant isozymes GFP-HAS2 and GFP-HAS3 travel through endoplasmic reticulum, Golgi, plasma membrne, and endocytic vesicles, expression of inactive GFP-tagged HAS3 D216A mutant and of GFP-tagged HAS3-deletion mutants in keratinocytes
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gene HAS1, expression analysis and recombinant expression in COS-1 cells
gene HAS1, in addition to the full-length form, HAS1 has multiple transcript variants resulting from alternative splicing
gene HAS1, quantitative real-time PCR isozyme expression analysis
gene HAS1, recombinant expression of GFP-tagged isozyme HAS1 in MCF-7 cells, MCF-7 cells transfected with Dendra-Has1 show intense fluorescence in perinuclear vesicular structures resembling the Golgi apparatus. The MCF-7 cells transfected with EGFP-Has1 produce a pericellular hyaluronan coat, which is attached the synthase itself
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gene HAS2, expression analysis and recombinant expression in COS-1 cells
gene has2, quantitative real time PCR enzyme expression analysis, HAS2 expression is dependent on ceramide generated by neutral type 2 sphingomyelinase, nSMase2, as shown by treatment with C2-ceramide that decreases HAS2 expression in murine fro/fro fibroblasts
gene HAS2, quantitative real-time PCR isozyme expression analysis
gene HAS2, real-time PCR enzyme expression
gene HAS3, expression analysis and recombinant expression in COS-1 cells
gene HAS3, quantitative real-time PCR isozyme expression analysis
gene hyaD, recombinant expression of the soluble C-terminally His6-tagged PmHAS1-703 truncated enzyme from pET101/D-TOPO expression vector with an additional V5 epitope
genes HAS1, HAS2 and HAS3, quantitative expression analysis in benign and malign endometrial tissue
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HAS1 DNA and amino acid sequence determination and analysis, oncogenic malignant transformation of 3Y1 fibroblasts with v-sre and/or v-fos, or v-HA-ras
HAS2 DNA and amino acid sequence determination and analysis, oncogenic malignant transformation of 3Y1 fibroblasts with v-sre and/or v-fos, or v-HA-ras
Homo sapiens hyaluronan synthase 1 is cloned into the plasmid pFLAG-CMV2, for in vitro translation the pF3A WG(BYDV) Flexi vector is used
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into a pCR2.1 vector for sequencing
isozyme HAS3 inducible overexpression in MV3/C8161 cells. EGFP-HAS3 overexpression rapidly induced pericellular hyaluronan coat formation and plasma membrane protrusions, induction by doxycycline
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overexpression in Escherichia coli, enzyme cannot be expressed as a soluble active protein
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overexpression of GFP-tagged HAS-1 in the wounds of lentiviral-HAS-1-treated mice
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recombinant expression of EGFP-tagged isozyme HAS1 in MCF-7 cells and localization to the Golgi apparatus
recombinant expression of EGFP-tagged isozyme HAS2 in MCF-7 cells and localization to the endoplasmic reticulum
recombinant expression of EGFP-tagged isozyme HAS3 in MCF-7 cells and localization to the Golgi apparatus
retroviral transduction system is used to overexpress the three murine hyaluronan synthase enzymes in arterial smooth muscle cells. Overexpression of hyaluronan synthases alters vascular smooth muscle cell phenotype and promotes monocyte adhesion
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stable expression of N-terminally Myc-tagged human HAS2 in NIH3T3 cells membranes, the molecular mechanism that underlies the rapid c-Myc-HAS2 turnover involves the 26 S proteasome, overview
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Streptococcus equisimilis hyaluronan synthase is cloned into the plasmid pKK223-3 for expression in Escherichia coli SURE cells
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the coding sequence of HAS1 is cloned into the vector pCR3.1 for transfection of fibroblasts
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the Escherichia coli expression vector pQE80L and the broad host range cloning vector pBBR122 are used
the human HAS2 expression plasmid is prepared by inserting its coding sequence into the vector pcDNA 3.1/CT-GFP-TOPO