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0.058 - 0.84
ADP-alpha-D-glucose
0.000164 - 2.5
ADPglucose
0.000864
glycogen
-
Arthrobacter glycogen
46.32
maltopentaose
isoform GBSSI, at pH 8.5 and 30°C
additional information
additional information
-
0.058
ADP-alpha-D-glucose
-
at pH 7.8, temperature not specified in the publication
0.09
ADP-alpha-D-glucose
isoform GBSSI, with maltobiose as cosubstrate, at pH 8.5 and 30°C
0.31
ADP-alpha-D-glucose
isoform SSIV, with maltotriose as cosubstrate, at pH 8.5 and 30°C
0.33
ADP-alpha-D-glucose
-
free isoform SSIII, at pH 8.0 and 30°C
0.39
ADP-alpha-D-glucose
-
free isoform SSI, at pH 8.0 and 30°C
0.44
ADP-alpha-D-glucose
isoform SSIIIb, with maltotriose as cosubstrate, at pH 8.5 and 30°C
0.63
ADP-alpha-D-glucose
isoform SSI with maltotriose as cosubstrate, at pH 8.5 and 30°C
0.84
ADP-alpha-D-glucose
-
free isoform SSIIa, at pH 8.0 and 30°C
0.1
ADP-glucose
-
mutant enzyme K193Q, glycogen as primer
0.11
ADP-glucose
-
wild-type enzyme, amylopectin as primer
0.13
ADP-glucose
-
wild-type enzyme, glycogen as primer
0.14
ADP-glucose
-
mutant enzyme K193Q, amylopectin as primer
0.15
ADP-glucose
-
mutant enzyme K193R, amylopectin as primer
0.15
ADP-glucose
-
mutant enzyme K193R, glycogen as primer
0.17
ADP-glucose
-
mutant enzyme K193E, glycogen as primer
0.22
ADP-glucose
-
mutant enzyme K193E, amylopectin as primer
0.22
ADP-glucose
pH 8.0, 30°C, at 10 mg/ml amylopectin
0.28
ADP-glucose
-
catalytic domain
0.32
ADP-glucose
pH 8.0, 30°C,at 10 mg/ml glycogen
0.59
ADP-glucose
-
catalytic domain co-expressed with starch-binding domain 3 and large part of starch-binding domain 2
0.62
ADP-glucose
-
catalytic domain co-expressed with starch-binding domains 23
0.81
ADP-glucose
-
catalytic domain co-expressed with starch-binding domain 2 and part of starch-binding domain 3
0.95
ADP-glucose
-
catalytic domain co-expressed with starch-binding domains 123
1.68
ADP-glucose
-
truncated protein lacking starch binding domain 1 and large part of starch binding domain 2
1.74
ADP-glucose
-
truncated protein lacking starch-binding domains 12
1.77
ADP-glucose
-
truncated protein lacking starch binding domain 1 and medium part of starch binding domain 2
2.39
ADP-glucose
-
truncated protein lacking starch binding domain 1 and small part of starch binding domain 2
2.56
ADP-glucose
-
truncated protein lacking starch-binding domain 1
4.08
ADP-glucose
-
wild-type
0.000164
ADPglucose
-
-
0.035
ADPglucose
-
glycogen synthases I and II
0.05
ADPglucose
-
granule-bound isoenzyme II from waxy maize
0.05 - 0.1
ADPglucose
-
-
0.053
ADPglucose
-
starch synthase I, primer amylopectin
0.07
ADPglucose
-
starch synthase II, 5 mg/ml amylopectin
0.09
ADPglucose
-
granule-bound isoenzyme I from waxy maize
0.1 - 0.12
ADPglucose
-
soluble isoenzymes
0.11
ADPglucose
-
granule-bound isoenzyme II
0.11
ADPglucose
-
isoenzyme II, primed reaction
0.11
ADPglucose
-
recombinant starch synthase IIa
0.11
ADPglucose
-
starch synthase I, primer amylopectin, 500 mM citrate
0.11 - 0.22
ADPglucose
-
-
0.12
ADPglucose
-
recombinant starch synthase IIb
0.14
ADPglucose
-
granule-bound isoenzyme I
0.15
ADPglucose
-
isoenzyme III
0.17
ADPglucose
-
isoenzyme I, primed reaction
0.18
ADPglucose
-
starch synthase I, presence of 500 mM citrate
0.2
ADPglucose
-
isoenzyme I
0.2
ADPglucose
-
isoenzyme IV, primed reaction
0.22
ADPglucose
-
isoenzyme III, primed reaction
0.24
ADPglucose
-
starch synthase I, absence of citrate
0.25
ADPglucose
-
isoenzyme IV
0.29
ADPglucose
-
isoenzyme II
0.29
ADPglucose
-
synthase I, absence of citrate
0.3
ADPglucose
-
starch synthase II, primer amylopectin, 500 mM citrate
0.48
ADPglucose
-
synthase II, absence of citrate
0.51
ADPglucose
-
synthase II, presence of citrate
0.97
ADPglucose
-
synthase I, presence of citrate
1.3
ADPglucose
-
granule bound starch synthase I, 5 mg/ml amylopectin
2.5
ADPglucose
-
assay in water or 85% isopropanol
0.015
maltotriose
-
-
15.3
maltotriose
-
recombinant granule-bound starch synthase I
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
-
amylopectin: 0.19-0.79 mg/ml, value depends on isozyme and presence of citrate
-
additional information
additional information
-
influence of citrate on values for primed and unprimed reaction
-
additional information
additional information
-
recombinant starch synthase IIa, amylopectin: 0.16 mg/ml, glycogen: 0.14 mg/ml, recombinant starch synthase IIb, amylopectin: 0.32 mg/ml, glycogen: 0.34 mg/ml
-
additional information
additional information
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0.002-1.5 mg/ml, value depends on isozyme and presence of citrate
-
additional information
additional information
-
E. coli B glycogen: 0.274 mg/ml, rabbit liver glycogen: 0.298 mg/ml, soluble potato amylose: 0.833 mg/ml
-
additional information
additional information
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amylose: 0.02-0.06 mg/ml, isoenzymes I, II and IV, amylopectin: 0.03-0.05 mg/ml, isoenzymes I, II and IV, rabbit liver glycogen: 0.8-1.02 mg/ml, isoenzymes I, II and IV, oyster glycogen: 1.18-6.66 mg/ml, isoenzymes I-IV, amylopectin beta-limit dextrin: 0.02-0.22 mg/ml, isoenzymes I-IV, glycogen E. coli beta-limit dextrin: 1.0-2.0 mg/ml, isoenzymes I-IV
-
additional information
additional information
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recombinant starch synthase I, amylopectin: 0.24 mg/ml, glycogen: 0.041 mg/ml
-
additional information
additional information
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granule-bound starch synthase I, amylpectin: 10 mg/ml, starch synthase II, amylopectin: 0.79 mg/ml
-
additional information
additional information
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starch synthase I, amylopectin: 0.08 mg/ml in the presence of citrate, 0.13 mg/ml in the absence of citrate, glycogen: 0.043 mg/ml in the presence of citrate, 12.8 mg/ml in the absence of citrate
-
additional information
additional information
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amylopectin: 0.16 mg/ml, rabbit liver glycogen: 1.16 mg/ml
-
additional information
additional information
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recombinant starch synthase II, amylopectin: 4.6 mg/ml
-
additional information
additional information
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Km-values for amylopectin: 0.2 mg/ml for wild-type enzyme, 0.53 mg/ml for mutant enzyme K193R, 0.13 mg/ml for mutant enzyme K193Q, 0.72 mg/ml for mutant enzyme K193E. Km-values for glycogen: 0.17 mg/ml for wild-type enzyme, 0.68 mg/ml for mutant enzyme K193R, 0.11 mg/ml for mutant enzyme K193Q, 0.53 mg/ml for mutant enzyme K193E
-
additional information
additional information
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kinetics, overview
-
additional information
additional information
Km-value for amylopectin is 2.4 mg/ml, KM-value for glycogen is 8.7 mg/ml
-
additional information
additional information
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Km-value for amylopectin is 2.4 mg/ml, KM-value for glycogen is 8.7 mg/ml
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