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2.4.1.21: starch synthase (glycosyl-transferring)

This is an abbreviated version!
For detailed information about starch synthase (glycosyl-transferring), go to the full flat file.

Word Map on EC 2.4.1.21

Reaction

ADP-alpha-D-glucose
+
[(1->4)-alpha-D-glucosyl]n
=
ADP
 +
[(1->4)-alpha-D-glucosyl]n+1

Synonyms

adenosine diphosphate glucose-starch glucosyltransferase, adenosine diphosphoglucose-starch glucosyltransferase, ADP-glucose starch synthase, ADP-glucose synthase, ADP-glucose transglucosylase, ADP-glucose-starch glucosyltransferase, ADP-glucose:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase, ADPG starch synthetase, ADPG-starch glucosyltransferase, AtSS2, AtSSII, AtSSIII, CkGBSS, dull1, GBSS, GBSSI, glgA, glucosyltransferase, adenosine diphosphoglucose-starch, glycogen synthase, glycogen/starch synthase, granule bound starch synthase, granule-bound starch synthase, granule-bound starch synthase I, GSase, OsGBSSII, OsSSIII-1, OsSSIII-2, OsSSIV-1, OsSSIV-2, PvSSI, PvSSII-1, SBD123, SS1, SS4, SSI, SSII, SSII(a), SSIIa, SSIIc, SSIII, SSIII(a), SSIII-1, SSIII-B, SSIII-C, SSIIIa, SSIIIb, SSIV, SSS, SSSI, SSV, starch synthase, starch synthase 1, starch synthase 4, starch synthase I, starch synthase II, starch synthase IIa, starch synthase III, starch synthase III-1, starch synthase III-2, starch synthase III-B, starch synthase IIIa, starch synthase IV, starch synthase IV-1, starch synthase IV-2, starch synthetase, starch-synthase III, starch/glycogen synthase, TaSSIVb, TVAG_258220

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.21 starch synthase (glycosyl-transferring)

Cloned

Cloned on EC 2.4.1.21 - starch synthase (glycosyl-transferring)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and sequencing of cDNA encoding SSIIa of japonica and indica rice cultivars, expression in Escherichia coli
-
cloning of starch synthase III cDNA
-
construction of recombinant full length and truncated isoforms of SSIII, lacking one, two, or three starch-binding domains, and recombinant proteins, containing three, two, or one starch-binding domains, to investigate the role of these domains in enzyme activity
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) pLysS cells
-
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
-
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells
-
expressed in Escherichia coli strain BL21(DE3) and in Saccharomyces cerevisiae strain WW100 lacking endogenous glycogen synthase activity
-
expressed in Escherichia coli Tuner(DE3) cells
expressed in Nicotiana benthamiana
-
expressed in Nicotiana benthamiana chloroplasts
-
expressed in Solanum lycopsericum
-
expressed in Synechocystis sp. PCC6803 mutants deficient in either glycogen synthase I or II
-
expression in Escherichia coli
expression of C-terminal domain in Escherichia coli
expression of full length and truncated starch synthase IIa and IIb in Escherichia coli
-
expression of full-length and truncated enzyme in Escherichia coli
-
expression of granule-bound starch synthase I and soluble starch synthase II in Escherichia coli
-
expression of granule-bound starch synthase I in Escherichia coli
-
expression of granule-bound starch synthase in Escherichia coli
-
expression of N-terminally His-tagged GSase in Escherichia coli strain BL21(DE3) or Top10
-
expression of OsSSIII-1 in Escherichia coli
expression of OsSSIII-2 in Escherichia coli
expression of OsSSIV-1 in Escherichia coli
expression of OsSSIV-2 in Escherichia coli
expression of PvSSI and PvSSII-1 in Escherichia coli
-
expression of starch synthase II in Escherichia coli and potato
-
isoform SSIII-B is expressed in Escherichia coli BL21(DE3) pLysS cells
-
the catalytic C-terminal domain (SSIII-CD) of is cloned and expressed in Escherichia coli. SSIII-CD fully complements the production of glycogen by an Agrobacterium tumefaciens glycogen synthase null mutant, suggesting that this truncated isoform restores in vivo de novo synthesis of bacterial glycogen
three recombinant proteins are constructed: putative mature recombinant SSIII (rPvSSII), recombinant kidney bean SSIII N-domain (rPvSSIII-N), and recombinant kidney bean SSIII C-domain (rPvSSIII-C)