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2.4.1.201: alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.201

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
=
UDP
+
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]

Synonyms

acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-glycopeptide beta-1,4-, VI, acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-glycopeptide beta-1-4-, VI, GCNT6, GnT VI, GnT-VI, mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, Mgat6, N-acetylglucosaminyltransferase VI, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase VI, UDP-GlcNAc: GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man] beta1-4N-acetylglucosaminyltransferase VI, UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha-R (GlcNAc to Man) beta4-GlcNAc-transferase VI, UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]beta1-4N-acetylglucosaminyltransferase VI, UDP-N-acetyl-D-glucosamine (GlcNAc):GlcNAcbeta1-6(GlcNAcbeta1-2)-Manalpha1-R[GlcNAc to Man]beta1,4N-acetylglucosaminyltransferase VI, uridine diphosphoacetylglucosamine-glycopeptide beta-1-4-acetylglucosaminyltransferase VI

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.201 alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Cloned

Cloned on EC 2.4.1.201 - alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from cDNA library, DNA sequence determination, functional expression in COS-1 cells
expressed in sf21 cells
-
gene MGAT6, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat6 HEK293 cells produce increased oligosaccharide levels also observed in HeLa cells, predicted to be the 2,2,4,6 tetra-, 4,2,2,4 tetra- and 4,2,2,4,6 penta-N-glycans, while native 4,2,2,6 tetra are reduced, N-glycans profiles of transgenic HeLa and HEK-293 cells, overview
-