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2.4.1.198: phosphatidylinositol N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about phosphatidylinositol N-acetylglucosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.198

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
1-phosphatidyl-1D-myo-inositol
=
UDP
 +
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol

Synonyms

acetyl-D-glucosaminyltransferase, uridine diphosphoacetylglucosamine alpha1,6-, afpig-a, Down syndrome critical region protein 5, DR437w, Dscr5, glycosylphosphatidylinositol N-acetylglucosaminyltransferase complex, glycosylphosphatidylinositol-N-acetylglucosaminyltransferase, GntA, GPI-GlcNAc transferase, GPI-GnT, GPI-N-acetylglucosaminyltransferase, GPI-N-acetylglucosaminyltransferase complex, GPI1, GPI15, GPI19, GPI2, GPI3, phosphatidylinositol N-acetylglucosaminyltransferase subunit A, phosphatidylinositol/UDP-GlcNAc:GlcNAc transferase, PIG-A, PigA, UDP-N-acetyl-D-glucosamine:phosphatidylinositol N-acetyl-D-glucosaminyltransferase, uridine diphosphoacetylglucosamine alpha1,6-acetyl-D-glucosaminyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.198 phosphatidylinositol N-acetylglucosaminyltransferase

Cloned

Cloned on EC 2.4.1.198 - phosphatidylinositol N-acetylglucosaminyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of FLAG-tagged PIG-C, GST-tagged PIG-A and PIG-H with and without mGPI1 in the mGPI1-deficient F9 cells
expressed in Daudi cells. Daudi cells have no PIG-Y transcritpt. PIG-Y cDNA restores the surface expression of CD59, DAF and CD48, but an empty vector does not. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase activity is also restored
expressed in Escherichia coli DH5alpha
expressed in HeLa cells
expression of FLAG-tagged and His-tagged PIG-A in PIG-A-deficient human B lymphoblastoid JY5 cells and in Escherichia coli, complementation in JY5 cells
-
expression of PIG-A/GPI3 and PIG-C/GPI2 as FLAG-tagged fusion proteins in yeast, expression of FLAG-tagged PIG-A/GPI3 in Escherichia coli
-
expression of PIG-P in enzyme deficient mouse mutant T cell line can complement and restore the enzyme activity
gene GPI1, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis
gene GPI15, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, phenotype, RT-PCR enzyme expression analysis
gene GPI19, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis
gene GPI2 encodes for one of the six accessory subunits of the GPI-N-acetylglucosaminyltransferase complex
gene GPI2, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, phenotype, RT-PCR enzyme expression analysis
gene GPI3, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis
gene gtA, functional recombinant expression of FLAG-tagged GntA enzyme in Saccharomyces cerevisiae
-
gene Pig-a, DNA and amino acid sequence determination and analysis
gene pigA, recombinant expression of codon-optimmized gene encoding the C-terminally His6-tagged wild-type and mutant enzymes with TEV protease cleavage site in Escherichia coli strain BL21(DE3)
genes involved in GPI-anchor synthesis in Caenorhabditis elegans, overview. Isolation and analysis of piga-1-knockout allele tm2939
-
hGPI1, DNA sequence determination, coexpression as GST-tagged protein in human B lymphoblastoid JY25 or JY5 cells with the other three GST- or FLAG-tagged protein of the active protein complex
in chinese hamster ovary cells
mGPI1, DNA sequence determination, expression in embryonal carcinoma F9 cells
overexpression of FLAG- and GST-double tagged PIG-A in human B lymphoblastoid JY5 cells