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0.122 - 15.5
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
558 - 1244
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
0.00183 - 0.0442
alpha-cyclodextrin
0.0095 - 0.014
beta-cyclodextrin
1612
cassava starch
-
pH 6.4, 55°C, formation of beta-cyclodextrin
-
0.000086 - 0.000133
L-ascorbic acid
1.66
p-nitrophenyl-(alpha-1,4-glucopyranosyl)2-D-glucose
-
E 192
0.833
p-nitrophenyl-(alpha-1,4-glucopyranosyl)3-D-glucose
-
E 192
0.833
p-nitrophenyl-(alpha-1,4-glucopyranosyl)4-D-glucose
-
E 192
0.833
p-nitrophenyl-(alpha-1,4-glucopyranosyl)5-D-glucose
-
E 192
0.833
p-nitrophenyl-(alpha-1,4-glucopyranosyl)6-D-glucose
-
E 192
0.25
p-nitrophenyl-(alpha-1,4-glucopyranosyl)7-D-glucose
-
E 192
70 - 73.4
soluble corn starch
-
24.8 - 803
soluble potato starch
-
1.86 - 3.5
soluble starch
-
additional information
additional information
-
-
-
0.122
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
mutant strain F283L, acceptor glucose, wild-type
0.137
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
strain 1011, acceptor maltose, mutant F283L
0.345
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
mutant strain F259L, acceptor glucose, wild-type
0.423
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
strain 1011, acceptor maltose, mutant F259L
1.97
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
mutant strain Y195L, acceptor glucose, wild-type
2.2
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
strain 1011, acceptor maltose, mutant Y195L
2.32
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
mutant strain Y195F, acceptor glucose, wild-type
2.63
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
strain 1011, acceptor maltose, mutant F183L/F259L
3.2
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
strain 1011, acceptor maltose, mutant Y195F
3.38
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
strain 1011, acceptor maltose, wild-type
3.8
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
mutant strain F183L/F259L, acceptor glucose, wild-type
4.07
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
strain 1011, acceptor glucose, wild-type
5.55
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
strain 1011, acceptor maltose, mutant F183L
15.5
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
-
mutant strain F183L, acceptor glucose, wild-type
558
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
-
pH 6.0, 60°C, mutant S77P
644
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
-
pH 6.0, 60°C, mutant W239R
1082
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
-
pH 6.0, 60°C, mutant W239L
1244
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
-
pH 6.0, 60°C, wild-type enzyme
0.00183
alpha-cyclodextrin
-
hydrolyzing activity, mutant Y195L
0.00383
alpha-cyclodextrin
-
hydrolyzing activity, mutant F283L
0.012
alpha-cyclodextrin
-
hydrolyzing activity, mutant F259L
0.0183
alpha-cyclodextrin
-
hydrolyzing activity, mutant F183L
0.0255
alpha-cyclodextrin
-
hydrolyzing activity, mutant Y195F
0.0442
alpha-cyclodextrin
-
hydrolyzing activity, wild-type
0.0095
beta-cyclodextrin
-
hydrolyzing activity, wild-type
0.014
beta-cyclodextrin
-
hydrolyzing activity, mutant Y195L
0.000086
L-ascorbic acid
-
mutant enzyme Y195S, at pH 5.5 and 37°C
0.000097
L-ascorbic acid
-
wild type enzyme, at pH 5.5 and 37°C
0.0001
L-ascorbic acid
-
mutant enzyme Q265K, at pH 5.5 and 37°C
0.000105
L-ascorbic acid
-
mutant enzyme Y260R, at pH 5.5 and 37°C
0.000106
L-ascorbic acid
-
mutant enzyme Q265K/Y195S, at pH 5.5 and 37°C
0.00011
L-ascorbic acid
-
mutant enzyme Y260R/Q265K, at pH 5.5 and 37°C
0.000114
L-ascorbic acid
-
mutant enzyme Y260R/Q265K/Y195S, at pH 5.5 and 37°C
0.000133
L-ascorbic acid
-
mutant enzyme Y260R/Y195S, at pH 5.5 and 37°C
0.004
maltodextrin
mutant K47W, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.004
maltodextrin
wild-type, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.005
maltodextrin
mutant K47F, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.005
maltodextrin
mutant K47V, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.0059
maltodextrin
-
wild type enzyme, at pH 5.5 and 37°C
0.006
maltodextrin
mutant K47L, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.0067
maltodextrin
-
mutant enzyme Y195S, at pH 5.5 and 37°C
0.0084
maltodextrin
-
mutant enzyme Q265K, at pH 5.5 and 37°C
0.009
maltodextrin
-
mutant enzyme Y260R, at pH 5.5 and 37°C
0.0096
maltodextrin
-
mutant enzyme Q265K/Y195S, at pH 5.5 and 37°C
0.0098
maltodextrin
-
mutant enzyme Y260R/Y195S, at pH 5.5 and 37°C
0.0104
maltodextrin
-
mutant enzyme Y260R/Q265K, at pH 5.5 and 37°C
0.0124
maltodextrin
-
mutant enzyme Y260R/Q265K/Y195S, at pH 5.5 and 37°C
0.079
maltodextrin
wild type enzyme, at pH 6.0 and 50°C
0.079
maltodextrin
mutant enzyme A156V/L174P/A166Y, at pH 6.0 and 50°C
280
maltodextrin
mutant enzyme L600Y, at pH 6.5 and 50°C
282
maltodextrin
wild type enzyme, at pH 6.5 and 50°C
285
maltodextrin
mutant enzyme L600I, at pH 6.5 and 50°C
299
maltodextrin
mutant enzyme L600E, at pH 6.5 and 50°C
358
maltodextrin
mutant enzyme L600R, at pH 6.5 and 50°C
371
maltodextrin
-
mutant enzyme D577G, at pH 6.5 and 50°C
371
maltodextrin
mutant enzyme D577G, at pH 6.5 and 50°C
403
maltodextrin
-
wild type enzyme, at pH 6.5 and 50°C
403
maltodextrin
wild type enzyme, at pH 6.5 and 50°C
406
maltodextrin
-
mutant enzyme D577A, at pH 6.5 and 50°C
406
maltodextrin
mutant enzyme D577A, at pH 6.5 and 50°C
467
maltodextrin
-
mutant enzyme D577V, at pH 6.5 and 50°C
467
maltodextrin
mutant enzyme D577V, at pH 6.5 and 50°C
513
maltodextrin
-
mutant enzyme D577I, at pH 6.5 and 50°C
513
maltodextrin
mutant enzyme D577I, at pH 6.5 and 50°C
583
maltodextrin
-
mutant enzyme D577L, at pH 6.5 and 50°C
583
maltodextrin
mutant enzyme D577L, at pH 6.5 and 50°C
768
maltodextrin
-
at pH 6.5 and 50°C
768
maltodextrin
-
at pH 6.5 and 50°C
558
maltose
-
pH 6.0, 60°C, mutant S77P
644
maltose
-
pH 6.0, 60°C, mutant W239R
1082
maltose
-
pH 6.0, 60°C, mutant W239L
1244
maltose
-
pH 6.0, 60°C, wild-type enzyme
70
soluble corn starch
wild type enzyme, at pH 6.0 and 60°C
-
71.37
soluble corn starch
mutant enzyme D355R, at pH 6.0 and 60°C
-
72
soluble corn starch
mutant enzyme R254F, at pH 6.0 and 60°C
-
73.4
soluble corn starch
mutant enzyme Y127F, at pH 6.0 and 60°C
-
24.8
soluble potato starch
-
mutant enzyme A137V, beta-cyclizing activity, at pH 6.4 and 55°C
-
127.5
soluble potato starch
-
mutant Q179L, pH 6.4, 56°C, calculated as production of beta-cyclodextrin
-
183.9
soluble potato starch
-
mutant enzyme L280A, beta-cyclizing activity, at pH 6.4 and 55°C
-
231.6
soluble potato starch
-
mutant enzyme M329I, beta-cyclizing activity, at pH 6.4 and 55°C
-
238.2
soluble potato starch
-
mutant enzyme A144V, beta-cyclizing activity, at pH 6.4 and 55°C
-
245.3
soluble potato starch
-
recombinant wild type enzyme, beta-cyclizing activity, at pH 6.4 and 55°C
-
246.2
soluble potato starch
-
wild-type, pH 6.4, 56°C, calculated as production of beta-cyclodextrin
-
247.2
soluble potato starch
-
mutant Q179G, pH 6.4, 56°C, calculated as production of beta-cyclodextrin
-
250.4
soluble potato starch
-
native wild type enzyme, beta-cyclizing activity, at pH 6.4 and 55°C
-
803
soluble potato starch
-
pH 6.4, 55°C, formation of beta-cyclodextrin
-
1.86
soluble starch
-
native enzyme, at pH 6.5 and 60°C
-
2.32
soluble starch
-
dextran-conjugated enzyme, at pH 6.5 and 60°C
-
2.6
soluble starch
mutant NS182G, pH 6.0, 60°C, calculated as production of beta-cyclodextrin
-
2.6
soluble starch
mutant S182E, pH 6.0, 60°C, calculated as production of beta-cyclodextrin
-
3.13
soluble starch
wild-type, pH 6.0, 60°C, calculated as production of beta-cyclodextrin
-
3.5
soluble starch
mutant N28R, pH 6.0, 60°C, calculated as production of beta-cyclodextrin
-
0.063
sophoricoside
wild type enzyme, at pH 6.0 and 50°C
0.063
sophoricoside
mutant enzyme A156V/L174P/A166Y, at pH 6.0 and 50°C
0.00267
starch
-
strain 1011, beta-cyclodextrin-forming activity, mutant F183L/F259L
0.035
starch
-
coupling activity, mutant F283L
0.122
starch
-
strain 1011, beta-cyclodextrin-forming activity, mutant F183L
0.137
starch
-
strain 1011, beta-cyclodextrin-forming activity, mutant Y195L
0.148
starch
-
strain 1011, beta-cyclodextrin-forming activity, mutant F259L
0.178
starch
-
coupling activity, mutant F259L
0.2
starch
-
strain 1011, beta-cyclodextrin-forming activity, mutant F283L
0.315
starch
-
coupling activity, mutant Y195L
0.482
starch
-
strain 1011, beta-cyclodextrin-forming activity, mutant Y195F
0.487
starch
-
coupling activity, mutant Y195F
0.508
starch
-
strain 1011, beta-cyclodextrin-forming activity, wild-type
0.818
starch
-
coupling activity, mutant F183L
1.13
starch
-
coupling activity, wild-type
1.33
starch
heated CGTase
1.51
starch
-
mutant E344D
1.66
starch
-
authentic CGTase
9
starch
pH 6.0, 60°C, beta-cyclization activity, mutant A230V
26
starch
pH 6.0, 60°C, beta-cyclization activity, mutant K232E
35
starch
pH 6.0, 60°C, beta-cyclization activity, mutant F283L
48
starch
pH 6.0, 60°C, beta-cyclization activity, mutant A230V/H140Q
79
starch
pH 6.0, 60°C, beta-cyclization activity, mutant H140Q
329
starch
pH 6.0, 60°C, beta-cyclization activity, wild-type enzyme