2.4.1.19: cyclomaltodextrin glucanotransferase
This is an abbreviated version!
For detailed information about cyclomaltodextrin glucanotransferase, go to the full flat file.
Word Map on EC 2.4.1.19
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2.4.1.19
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cyclodextrins
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cgtases
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starch
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synthesis
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transglycosylation
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circulans
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cyclization
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glucosylated
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glucoamylase
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paenibacillus
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nutrition
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beta-cyclodextrins
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alkalophilic
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gamma-cyclodextrins
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beta-cds
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alpha-amylases
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maltooligosaccharides
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stearothermophilus
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disproportionation
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amylose
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maltodextrins
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gamma-cds
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industry
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starch-degrading
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maltohexaose
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starch-binding
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amylopectin
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amylolytic
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cyclomaltohexaose
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agriculture
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alpha-cd
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maltopentaose
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food industry
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pharmacology
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thermoanaerobacter
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maltotetraose
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maltotriose
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beta-amylase
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amyloglucosidase
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pullulanase
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alpha-1,4-glucans
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thermosulfurigenes
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acarbose
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thermoanaerobacterium
- 2.4.1.19
- cyclodextrins
- cgtases
- starch
- synthesis
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transglycosylation
- circulans
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cyclization
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glucosylated
- glucoamylase
- paenibacillus
- nutrition
- beta-cyclodextrins
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alkalophilic
- gamma-cyclodextrins
- beta-cds
- alpha-amylases
- maltooligosaccharides
- stearothermophilus
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disproportionation
- amylose
- maltodextrins
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gamma-cds
- industry
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starch-degrading
- maltohexaose
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starch-binding
- amylopectin
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amylolytic
- cyclomaltohexaose
- agriculture
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alpha-cd
- maltopentaose
- food industry
- pharmacology
- thermoanaerobacter
- maltotetraose
- maltotriose
- beta-amylase
- amyloglucosidase
- pullulanase
- alpha-1,4-glucans
- thermosulfurigenes
- acarbose
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thermoanaerobacterium
Reaction
Synonyms
1,4-alpha-D-glucopyranosyl transferase, Akrilex C cyclodextrin glycosyltransferase, alpha-1,4-glucan 4-glycosyltransferase, cyclizing, alpha-cgt, alpha-CGTase, alpha-cyclodextrin glucanotransferase, alpha-cyclodextrin glucosyltransferase, alpha-cyclodextrin glycosyltransferase, Bacillus macerans amylase, beta-CGTase, beta-cyclodextrin glucanotransferase, beta-cyclodextrin glucosyltransferase, beta-cyclodextrin glycosyltransferase, beta-cyclodextrinase, BMA, C-CGTase, CD glucanotransferase, CGT, CGT13, CGTase, cgtS, CGTse ET1, CGT_TK, cyclodextrin beta-glucanotransferase, cyclodextrin glucanotransferase, cyclodextrin glucosyltransferase, cyclodextrin glycosyl transferase, cyclodextrin glycosyltransferase, cyclodextrin-glycosyltransferase, cyclodextrinase, cyclomaltodextrin glucanyltransferase, cyclomaltodextrin glucotransferase, cyclomaltodextrin glycanotransferase, cyclomaltodextrin glycosyltransferase, gamma-CGTase, gamma-cyclodextrin glycosyltransferase, konchizaimu, M-CGTase, More, neutral-cyclodextrin glycosyltransferase, PFCGT, Toruzyme
ECTree
2.4.1.19 cyclomaltodextrin glucanotransferaseAdvanced search results
results (
results (KM Value
KM Value on EC 2.4.1.19 - cyclomaltodextrin glucanotransferase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.08
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor maltose, mutant F183L
0.12
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor maltose, mutant F283L
0.15
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor glucose, mutant F283L
0.16
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor maltose, wild-type
0.2
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor glucose, wild-type and mutant Y195F
0.22
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor maltose, mutant Y195F
0.23
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor glucose, mutant F183L
0.24
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor maltose, mutant F183L/F259L
0.31
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor maltose, mutant Y195L
0.34
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor glucose, mutant Y195L and F183L/F259L
0.48
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor maltose, mutant F259L
0.56
3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside
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strain 1011, acceptor glucose, mutant F259L
0.15
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
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pH 6.0, 60°C, wild-type enzyme
0.2
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
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pH 6.0, 60°C, mutant W239R
0.24
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
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pH 6.0, 60°C, mutant S77P
0.27
4,6-O-ethylidene-4-nitrophenyl alpha-D-maltoheptaoside
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pH 6.0, 60°C, mutant W239L
0.1
gamma-cyclodextrin
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coupling activity, mutant Y195F and F283L
7.3
glucose
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strain 1011, acceptor glucose, disproportionation, F283L
9.8
glucose
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strain 1011, acceptor glucose, disproportionation, Y195L
11.3
glucose
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strain 1011, acceptor glucose, disproportionation, wild-type
12
glucose
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strain 1011, acceptor glucose, disproportionation, F259L
15.9
glucose
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strain 1011, acceptor glucose, disproportionation, Y195F
57.8
glucose
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strain 1011, acceptor glucose, disproportionation, F183L
160
glucose
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strain 1011, acceptor glucose, disproportionation, F183L/F259L
38.3
L-ascorbic acid
wild-type, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
44.7
L-ascorbic acid
mutant K47W, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
46.1
L-ascorbic acid
mutant K47F, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
46.9
L-ascorbic acid
mutant K47V, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
51.07
L-ascorbic acid
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mutant enzyme Y260R/Q265K/Y195S, at pH 5.5 and 37°C
51.7
L-ascorbic acid
mutant K47L, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.47
maltodextrin
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mutant enzyme Y260R/Q265K/Y195S, at pH 5.5 and 37°C
0.48
maltodextrin
mutant K47V, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.49
maltodextrin
mutant K47L, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.52
maltodextrin
mutant K47F, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.55
maltodextrin
mutant K47W, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.64
maltodextrin
wild-type, pH 5.5, 37°C, average molecular weight of 2150.68 Da for maltodextrin
0.17
maltose
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strain 1011, acceptor maltose, disproportionation, F283L
0.38
maltose
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strain 1011, acceptor maltose, disproportionation, Y195L
0.59
maltose
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strain 1011, acceptor maltose, disproportionation, Y195F
1.3
maltose
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strain 1011, acceptor maltose, disproportionation, F259L
1.42
maltose
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strain 1011, acceptor maltose, disproportionation, F183L
18.2
maltose
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strain 1011, acceptor maltose, disproportionation, F183L/F259L
0.0556
starch
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strain 1011, beta-cyclodextrin-forming activity, mutant Y195F
0.0574
starch
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strain 1011, beta-cyclodextrin-forming activity, wild-type
0.0618
starch
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strain 1011, beta-cyclodextrin-forming activity, mutant F259L
0.0635
starch
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strain 1011, beta-cyclodextrin-forming activity, mutant Y195L
0.0724
starch
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strain 1011, beta-cyclodextrin-forming activity, mutant F283L
0.0815
starch
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strain 1011, beta-cyclodextrin-forming activity, mutant F183L
0.263
starch
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strain 1011, beta-cyclodextrin-forming activity, mutant F183L/F259L
additional information
additional information
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reaction kinetics
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additional information
additional information
reaction kinetics
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additional information
additional information
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reaction kinetics
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additional information
additional information
reaction kinetics
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additional information
additional information
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Michaelis-Menten kinetics, overview
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additional information
additional information
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Km-value for starch: 0.0025 mg/ml (soluble enzyme), 0.0045 mg/ml (enzyme immobilized on alginate)
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additional information
additional information
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kinetics of hydrolyzing activity with different substrates, e.g. cyclodextrins, overview
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additional information
additional information
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kinetics of the four reaction steps, overview
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additional information
additional information
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dynamics of beta-cyclodextrin production, kinetics, overview
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additional information
additional information
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kinetics of His-tagged wild-type and mutant enzymes, overview
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additional information
additional information
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kinetic data fit to Hill equation and lead to coefficients of 1.485 and 1.257 for the recombinant and the native enzyme, respectively
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additional information
additional information
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Km value is 0.69 mg/ml for wild-type, 0.57 mg/ml for mutant Q179G, 0.54 mg/ml for mutant Q179l, substrate soluble potato starch, pH 6.4, 56°C
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additional information
additional information
Km value is 17.2 mg/ml for beta-cyclization activity using soluble starch, pH 7.0, 55°C
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additional information
additional information
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Km value is 17.2 mg/ml for beta-cyclization activity using soluble starch, pH 7.0, 55°C
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