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(CH3COO)2Pb
Bacillus autolyticus
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relative activity 103%
2-mercaptoethanol
Bacillus autolyticus
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relative activity 101%
Benzene
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relative activity 106%
Ca2+
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4 mM, 22% activation
chitosan
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the beta-CGTase and gamma-CGTase activities increase as the chitosan concentrations increases to 2% (w/v)
decane
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increases the yield of cyclodextrin
Dextrin
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favores CGTase synthesis
dithiothreitol
Bacillus autolyticus
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relative activity 101%
ethylene glycol
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with increasing concentrations of ethylene glycol (up to 20% v/v), the beta-CGTase activity increases by 9.8%, 65.4%, and 35.1%, respectively, and the gamma-CGTase activity increases by 7.8%, 66.7%, and 39.0%, respectively, compared with that of control
fructose
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favores CGTase synthesis
galactose
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favores CGTase synthesis
gelatin
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with increasing gelatin concentrations, both the beta-CGTase and gamma-CGTase activities show bell-shaped curves, with the highest increases of activities of 48.9% and 38.6%, respectively, at 1% (w/v) gelatin
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glucose
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favores CGTase synthesis
glycerol
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with increasing concentrations of glycerol (up to 20% v/v), the beta-CGTase activity increases by 9.8%, 65.4%, and 35.1%, respectively, and the gamma-CGTase activity increases by 7.8%, 66.7%, and 39.0%, respectively, compared with that of control
lactose
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favores CGTase synthesis
light
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illumination of the enzyme with white, linearly polarized light and at constant 25°C increases the enzyme activity producing a mixture of alpha-, beta-, and gamma-cyclodextrins. At a high enzyme concentration of 0.64 U/cm3, regardless the illumination time, formation of beta-cyclodextrin predominates. The highest yield of beta-cyclodextrin is afforded after 1 h illumination, but 2 h illumination leads to a significant increase in the yield of gamma-cyclodextrin
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maltodextrin
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favores CGTase synthesis
maltose
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favores CGTase synthesis
mannitol
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favores CGTase synthesis
mycose
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the beta-CGTase and gamma-CGTase activities increase as the mycose concentrations increases to 2% (w/v)
NaCl
enzyme shows its maximum hydrolysis activity in a buffer with 1.5 M NaCl and retains up to 65% of its activity at 0.5 M NaCl
p-chloromercuribenzoic acid
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80 mM, relative activity 118%
PEG 3000
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10% w/v, increases cyclodextrin production
PEG400
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the beta-CGTase and gamma-CGTase activities increase by 50.4% and 55.3% compared with that of control at the condition of 10% (w/v) PEG400
phenylmethylsulfonyl fluoride
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80 mM, relative activity 114%
Sodium acetate
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the highest increase in beta-CGTase activity is 46.9% at 1 mM sodium acetate which is 5.8fold higher than that of the control. The highest increase in gamma-CGTase activity is 39.1% at 2 mM sodium acetate which is 5.0fold higher than that of the control
starch
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favores CGTase synthesis
sucrose
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favores CGTase synthesis
tert-butanol
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strain 251, increases the yield of cyclodextrin
Triton X-100
Bacillus autolyticus
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enhances specificity and yield of beta-cyclodextrin production
xylose
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favores CGTase synthesis
additional information
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synergistic promoting effects of glycine and Ca2+ on the extracellular secretion of the recombinant protein in Escherichia coli, optimal at 150 mM and 20 mM, respectively, overview
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bromobenzene
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increases the yield of beta-cyclodextrin
bromobenzene
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increases the yield of beta-cyclodextrin
bromobenzene
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increases the yield of beta-cyclodextrin
bromobenzene
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increases the yield of beta-cyclodextrin
bromobenzene
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increases the yield of beta-cyclodextrin
bromobenzene
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increases the yield of beta-cyclodextrin
cyclohexadec-8-ene-1-one
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increases the yield of gamma-cyclodextrin
cyclohexadec-8-ene-1-one
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increases the yield of gamma-cyclodextrin
cyclohexadec-8-ene-1-one
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increases the yield of gamma-cyclodextrin
cyclohexadec-8-ene-1-one
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increases the yield of gamma-cyclodextrin
cyclohexadec-8-ene-1-one
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increases the yield of gamma-cyclodextrin
cyclohexadec-8-ene-1-one
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increases the yield of gamma-cyclodextrin
Cyclohexane
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increases the yield of beta-cyclodextrin
Cyclohexane
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increases the yield of beta-cyclodextrin
Cyclohexane
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increases the yield of beta-cyclodextrin
Cyclohexane
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relative activity 102%
Cyclohexane
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increases the yield of beta-cyclodextrin
Cyclohexane
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increases the yield of beta-cyclodextrin
Cyclohexane
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increases the yield of beta-cyclodextrin
cyclotridecanone
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increases the yield of gamma-cyclodextrin
cyclotridecanone
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increases the yield of gamma-cyclodextrin
cyclotridecanone
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increases the yield of gamma-cyclodextrin
cyclotridecanone
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increases the yield of gamma-cyclodextrin
cyclotridecanone
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increases the yield of gamma-cyclodextrin
cyclotridecanone
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increases the yield of gamma-cyclodextrin
Decanol
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increases the yield of alpha-cyclodextrin
Decanol
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increases the yield of alpha-cyclodextrin
Decanol
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increases the yield of alpha-cyclodextrin
Decanol
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increases the yield of alpha-cyclodextrin
Decanol
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increases the yield of alpha-cyclodextrin
Decanol
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increases the yield of alpha-cyclodextrin
EDTA
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80 mM, relative activity 117%
EDTA
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10 mM, 105% of initial activity
EDTA
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about 110% activity at 10 mM
ethanol
Bacillus autolyticus
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enhances specificity and yield of beta-cyclodextrin production
ethanol
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increases the yield of alpha-cyclodextrin
ethanol
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enhances the formaton of gamma-cyclodextrin
ethanol
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BE101 produces twice as much cyclodextrin
ethanol
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increases the yield of alpha-cyclodextrin
ethanol
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increases the yield of alpha-cyclodextrin
ethanol
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increases yield of cyclodextrins, relative activity 104%
ethanol
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increases the yield of alpha-cyclodextrin
ethanol
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increases cyclodextrin yield
ethanol
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increases the yield of alpha-cyclodextrin
ethanol
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increases the yield of alpha-cyclodextrin
Isopropanol
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increases the yield of beta-cyclodextrin
Isopropanol
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increases the yield of beta-cyclodextrin
Isopropanol
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increases the yield of beta-cyclodextrin
Isopropanol
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increases the yield of beta-cyclodextrin
Isopropanol
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increases the yield of beta-cyclodextrin
Isopropanol
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increases the yield of beta-cyclodextrin
Isopropanol
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increases the yield of beta-cyclodextrin
n-butanol
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increases the yield of alpha-cyclodextrin
n-butanol
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increases the yield of alpha-cyclodextrin
n-butanol
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increases the yield of alpha-cyclodextrin
n-butanol
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increases the yield of alpha-cyclodextrin
n-butanol
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increases the yield of alpha-cyclodextrin
n-butanol
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increases the yield of alpha-cyclodextrin
Octanol
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increases the yield of alpha-cyclodextrin
Octanol
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increases the yield of alpha-cyclodextrin
Octanol
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increases the yield of alpha-cyclodextrin
Octanol
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increases the yield of alpha-cyclodextrin
Octanol
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increases the yield of alpha-cyclodextrin
Octanol
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increases the yield of alpha-cyclodextrin
pentadecane
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increases the yield of beta-cyclodextrin
pentadecane
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increases the yield of beta-cyclodextrin
pentadecane
-
increases the yield of beta-cyclodextrin
pentadecane
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increases the yield of beta-cyclodextrin
pentadecane
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increases the yield of beta-cyclodextrin
pentadecane
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increases the yield of beta-cyclodextrin
sorbitol
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favores CGTase synthesis
sorbitol
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1 mM, increases cyclodextrin production
Toluene
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increases the yield of beta-cyclodextrin
Toluene
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increases the yield of beta-cyclodextrin
Toluene
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increases the yield of beta-cyclodextrin
Toluene
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relative residual activity 102%, relative activity 104%
Toluene
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increases the yield of beta-cyclodextrin
Toluene
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increases the yield of beta-cyclodextrin
Toluene
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increases the yield of beta-cyclodextrin
Toluene
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increases the yield of beta-cyclodextrin
Trichloroethylene
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increases the yield of beta-cyclodextrin
Trichloroethylene
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increases the yield of beta-cyclodextrin
Trichloroethylene
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increases the yield of beta-cyclodextrin
Trichloroethylene
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increases the yield of beta-cyclodextrin
Trichloroethylene
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increases the yield of beta-cyclodextrin
Trichloroethylene
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increases the yield of beta-cyclodextrin
Trichloroethylene
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increases the yield of beta-cyclodextrin
undecane
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increases the yield of beta-cyclodextrin
undecane
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increases the yield of beta-cyclodextrin
undecane
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increases the yield of beta-cyclodextrin
undecane
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increases the yield of beta-cyclodextrin
undecane
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increases the yield of beta-cyclodextrin
undecane
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increases the yield of beta-cyclodextrin