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2.4.1.186: glycogenin glucosyltransferase

This is an abbreviated version!
For detailed information about glycogenin glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.186

Reaction

UDP-alpha-D-glucose
+
glycogenin
=
UDP
+
alpha-D-glucosylglycogenin

Synonyms

CeGN, CgGN, CgGN-alpha, CgGN-beta, CgGN-gamma, EC 2.4.1.112, glucosyltransferase, uridine diphosphoglucose-protein, glucosyltransferase, uridine diphosphoglucose-protein 4-alpha-, glycogen initiator synthase, glycogenin, glycogenin 1, glycogenin 2, glycogenin glycosyltransferase, glycogenin-1, glycogenin-2, glyogenin, GN-1, GN1, GN2, GNN, GYG, GYG1, GYG2, hGYG1, M-glycogenin, priming glucosyltransferase, proglycogen synthase, UDP-glucose protein transglucosylase, UDP-glucose-protein glucosyltransferase, UDP-glucose:protein glucosyltransferase, UDPGlc:protein transglucosylase, UPTG, uridine diphosphate glucose-protein transglucosylase I, uridine diphosphoglucose protein transglucosylase I, uridine diphosphoglucose-protein 4-alpha-glucosyltransferase, uridine diphosphoglucose-protein glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.186 glycogenin glucosyltransferase

Purification

Purification on EC 2.4.1.186 - glycogenin glucosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-Sepharose column chromatography and Q Sepharose column chromatography
-
nickel-NTA column and Q-Sepharose column
-
recombinant GST-tagged glycogen synthase in complex with glycogenin-1 from Sf9 insect cells by glutathione affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from cell-free expression by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography
recombinant His6-tagged wild-type and mutant glycogenin-1 from HEK-293 cells by nickel affinity chromatography
recombinant His6-tagged wild-type and mutant glycogenin-2 from HEK-293 cells by nickel affinity chromatography
recombinant proteins
recombinant wild-type gycogenin-1, its truncated mutant DELTA-270, and of glycogenin-1 point mutants from Escherichia coli strain CGSC 4997
separation from glycogen synthase, EC 2.4.1.11, by LiBr
-
separation from proteoglycogen
wild-type and mutant enzymes