2.4.1.183: alpha-1,3-glucan synthase
This is an abbreviated version!
For detailed information about alpha-1,3-glucan synthase, go to the full flat file.
Word Map on EC 2.4.1.183
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2.4.1.183
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glucans
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beta-1,3-glucan
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water-insoluble
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rho1
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brasiliensis
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sobrinus
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dimorphic
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gtpases
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polysaccharide
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paracoccidioides
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niger
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mutans
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nidulans
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1,6-alpha-d-glucan
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dextran
- 2.4.1.183
- glucans
- beta-1,3-glucan
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water-insoluble
- rho1
- brasiliensis
- sobrinus
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dimorphic
- gtpases
- polysaccharide
- paracoccidioides
- niger
- mutans
- nidulans
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1,6-alpha-d-glucan
- dextran
Reaction
Synonyms
1,3-alpha-D-glucan synthase, AbagsA, AbagsB, AbagsC, AbagsD, AbagsE, AGS, Ags1, AGS2, AGS3, agsA, agsB, agsC, agsE, alpha-1,3-glucan synthase, glucosyltransferase, uridine diphosphoglucose-1,3-alpha-glucan, PcagsA, PcagsB, PcagsC, uridine diphosphoglucose-1,3-alpha-glucan glucosyltransferase
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General Information
General Information on EC 2.4.1.183 - alpha-1,3-glucan synthase
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evolution
AgsE is one of five alpha-1,3-glucan synthase genes in Aspergillus luchuensis and a homologue of the major alpha-1,3-glucan synthase agsB in Aspergillus nidulans
malfunction
physiological function
additional information
alpha-1,3-glucan is the main component of the alkali-soluble cell wall fraction in the wild-type and agsA disruption strains, but almost no alpha-1,3-glucan is found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Hyphal morphology of the control and agsB disruption strains, overview
malfunction
deletion of all the three AGS genes results in a triple mutant that is devoid of alpha-(1,3)-glucan in its cell wall, buts growth and germination is identical to that of the parental strain in vitro. In the experimental murine aspergillosis model, this mutant is less pathogenic than the parental strain. The AGS deletion results in an extensive structural modification of the conidial cell wall, especially conidial surface where the rodlet layer is covered by an amorphous glycoprotein matrix. The surface modification is responsible for viability reduction of conidia in vivo, which explains decrease in the virulence of triple agsD mutant
malfunction
deletion of all the three AGS genes results in a triple mutant that is devoid of alpha-(1,3)-glucan in its cell wall, buts growth and germination is identical to that of the parental strain in vitro. In the experimental murine aspergillosis model, this mutant is less pathogenic than the parental strain. The AGS deletion results in an extensive structural modification of the conidial cell wall, especially conidial surface where the rodlet layer is covered by an amorphous glycoprotein matrix. Thie surface modification is responsible for viability reduction of conidia in vivo, which explains decrease in the virulence of triple agsD mutant
malfunction
disruption of agsE in Aspergillus luchuensis strain NBRC 4314 (DELTAagsE) shows that protoplast formation in DELTAagsE is comparable with protoplast formation in Aspergillus oryzae with commercial cell wall-digesting enzyme Yatalase. The DELTAagsE protoplasts are also competent for transformation with the protoplast-PEG method
malfunction
disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain
malfunction
disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain
malfunction
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overexpression of Aspergillus nidulans alpha-1,3-glucan synthase increases cellular adhesion and causes cell wall defects
malfunction
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the disruption of the agsA gene does not alter or slightly increase the alpha-1,3-glucan content compared to wild-type. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
malfunction
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the disruption of the agsB gene leads to loss of alpha-1,3-glucan. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
malfunction
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the disruption of the agsB gene leads to the loss of alpha-1,3-glucan. Overexpression of agsB increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan. The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains
malfunction
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alpha-1,3-glucan is the main component of the alkali-soluble cell wall fraction in the wild-type and agsA disruption strains, but almost no alpha-1,3-glucan is found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Hyphal morphology of the control and agsB disruption strains, overview
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malfunction
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the disruption of the agsA gene does not alter or slightly increase the alpha-1,3-glucan content compared to wild-type. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
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malfunction
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the disruption of the agsB gene leads to loss of alpha-1,3-glucan. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
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malfunction
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disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain
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malfunction
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disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain
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malfunction
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disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain
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malfunction
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disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain
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the enzyme is involved in biosynthesis of alpha-1,3-glucan and the cell wall biogenesis. alpha-(1,3)-Glucan is a major cell wall component of most ascomycetous and basidiomycetous fungi, including the human pathogens. In Aspergillus fumigatus, alpha-(1,3)-glucan is a key component of the extracellular matrix, which encloses the cell wall beta-(1,3)-glucan-chitin fibrillar core
physiological function
the enzyme is involved in the cell wall biogenesis. Alteration of the alpha-1,3-glucan amount is counterbalanced by alterations in the amount of another cell wall component such as beta-1,3-glucan or chitin
physiological function
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alpha-1,3-glucan has important roles in fungal cellular adhesion and may contribute to fungal pathogenesis
physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular
physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions
physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions
physiological function
role of the alpha-1,3-glucan synthase gene agsE in protoplast formation: agsE appears to inhibit protoplast formation in Aspergillus luchuensis, overview
physiological function
the ags genes play a major role in alpha-1,3-glucan biosynthesis in Aspergillus oryzae, while the amyD and amyG genes do not
physiological function
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the enzyme is involved in the cell wall biogenesis. Alteration of the alpha-1,3-glucan amount is counterbalanced by alterations in the amount of another cell wall component such as beta-1,3-glucan or chitin
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physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular
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physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions
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physiological function
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the ags genes play a major role in alpha-1,3-glucan biosynthesis in Aspergillus oryzae, while the amyD and amyG genes do not
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physiological function
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the ags genes play a major role in alpha-1,3-glucan biosynthesis in Aspergillus oryzae, while the amyD and amyG genes do not
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expression profiles of genes involved in cell wall biosynthesis. Levels of transcription of several cell wall-related genes (gelA, chsA, and gfaA) are significantly upregulated in the agsB disruption strain, transcription of gelB and csmB is significantly downregulated in the disruptant. In the CagsB strain, the expression of genes agsA, fksA, gelA, chsA, and gfaA is significantly upregulated under the agsB-repressing conditions, as is the case in the agsB disruption strain for all except fksA. In contrast, under the agsB-inducing conditions, the expression levels of fksA, gelB, chsD, and gfaA are significantly reduced in the CagsB strain, gene csmA is significantly upregulated under these conditions
additional information
analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
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determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the inner layer in the agsA overexpressing strain
additional information
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determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the outer layer of the cell wall in the agsB overexpressing strain
additional information
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determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the outer layer of the cell wall in the agsB overexpressing strain
additional information
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expression profiles of genes involved in cell wall biosynthesis. Levels of transcription of several cell wall-related genes (gelA, chsA, and gfaA) are significantly upregulated in the agsB disruption strain, transcription of gelB and csmB is significantly downregulated in the disruptant. In the CagsB strain, the expression of genes agsA, fksA, gelA, chsA, and gfaA is significantly upregulated under the agsB-repressing conditions, as is the case in the agsB disruption strain for all except fksA. In contrast, under the agsB-inducing conditions, the expression levels of fksA, gelB, chsD, and gfaA are significantly reduced in the CagsB strain, gene csmA is significantly upregulated under these conditions
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additional information
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determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the inner layer in the agsA overexpressing strain
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additional information
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analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
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additional information
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analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
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