2.4.1.174: glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase

This is an abbreviated version!
For detailed information about glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase, go to the full flat file.

Reaction

Synonyms

acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-chondroitin, I, ChGn-1, ChGn-2, chondroitin beta1,4-N-acetylgalactosaminyltransferase-1, chondroitin galactosaminyltransferase, chondroitin N-acetylgalactosaminyltransferase-1, chondroitin N-acetylgalactosaminyltransferase-2, chondroitin sulfate beta-1,4-N-acetylgalactosaminyltransferase-1 ChGn-1, chondroitin sulfate N-acetylgalactosaminyltransferase 1, chondroitin sulfate N-acetylgalactosaminyltransferase 2, chondroitin sulfate N-acetylgalactosaminyltransferase-1, chondroitin sulfate N-acetylgalactosaminyltransferase-2, chondroitin sulfate synthase 1, chondroitin sulfate synthase-1, chondroitin sulphate N-acetylgalactosaminyl-transferase-1, chondroitin sulphate N-acetylgalactosaminyltransferase 1, CHSY1, CS N-acetylgalactosaminyltransferase 1, CS N-acetylgalactosaminyltransferase-1, CSGal-NACT-1, CSGalNAcT-1, CSGalNAcT1, CSGALNACT2, CSS1, GalNAcT-I, GalNAcT-II, glucuronylgalactosylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase, More, N-acetylgalactosamineT-II, N-acetylgalactosaminyltransferase I, uridine diphosphoacetylgalactosamine-chondroitin acetylgalactosaminyltransferase I

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.174 glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase

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Results	in table
9	AA Sequence
2	Application
1	CAS Registry Number
21	Cloned(Commentary)
32	Disease/ Diagnostics
6	Expression
67	General Information
39	Inhibitors
10	Localization
5	Metals/Ions
3	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
110	Organism
5	Pathway
5	pH Optimum
2	Posttranslational Modification
26	Protein Variants
2	Purification (Commentary)
2	Reaction
1	Reaction Type
35	Reference
112	Source Tissue
2	Specific Activity [micromol/min/mg]
42	Substrates and Products (Substrate)
3	Subunits
83	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Stability [°C]

Substrates Products

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Substrates Products on EC 2.4.1.174 - glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan

7 entries

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan

6 entries

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactose

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactose

Bos taurus

-

minimum requirement for acceptor structure

638344

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactose

UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactose

Bos taurus

-

best substrate

638344

-

638344

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(Gly)Ser-(Gly-Glu)

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(Gly)Ser-(Gly-Glu)

Homo sapiens

Q8N6G5, Q8TDX6

-

638347

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser

Homo sapiens

Q8N6G5, Q8TDX6

-

638347

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser-peptide

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser-peptide

Homo sapiens

Q8N6G5, Q8TDX6

-

638347

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl

3 entries

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose

Bos taurus

-

-

638344

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl

2 entries

UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

2 entries

UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin

UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin

2 entries

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides

4 entries

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides

4 entries

additional information

?

-

6 entries

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan

Homo sapiens

Q8TDX6

-

759329

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan

Homo sapiens

Q8N6G5, Q8TDX6

-

759849

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan

Homo sapiens

Q8TDX6

-

760038

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan

Mus musculus

Q8BJQ9

-

759758, 759849

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan

Mus musculus

Q8C1F4

-

759849

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan

Mus musculus

Q8BJQ9, Q8C1F4

-

760033

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan

Mus musculus C57BL/6

Q8BJQ9, Q8C1F4

-

760033

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan

Danio rerio

A8E7C8, E7F6G2

-

735999

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan

Homo sapiens

-

-

722363

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan

Homo sapiens

Q8TDX6

-

736890

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan

Mus musculus

-

-

721534, 722738, 723586

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan

Mus musculus

Q8BJQ9

-

736871

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan

UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan

Mus musculus C57/BL6J

-

-

722738

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl

Homo sapiens

Q8TDX6

i.e. linkage tetrasaccharide, synthetic substrate

638345

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl

Homo sapiens

Q8TDX6

best acceptor substrate together with chondroitin polysaccharide

638345

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl

Homo sapiens

Q8TDX6

transfers GalNAc to GlcA at the non-reducing terminus of the linkage tetrasacharide

638345

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl

Homo sapiens

Q8TDX6

-

638346

-

-

?

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl

UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl

Homo sapiens

Q8N6G5, Q8TDX6

-

638347

-

-

?

UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

Mus musculus

Q8BJQ9, Q8C1F4

a phosphorylated tetrasaccharide linkage structure in ChGn-1-/- growth plate cartilage but not in ChGn-2-/- or wild-type growth plate cartilage, preferred substrate of ChGn-1

736493

-

-

?

UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

Mus musculus C57BL/6

Q8BJQ9, Q8C1F4

a phosphorylated tetrasaccharide linkage structure in ChGn-1-/- growth plate cartilage but not in ChGn-2-/- or wild-type growth plate cartilage, preferred substrate of ChGn-1

736493

-

-

?

UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin

UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin

Mus musculus

Q8BJQ9, Q8C1F4

-

736493

-

-

?

UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin

UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin

Mus musculus C57BL/6

Q8BJQ9, Q8C1F4

-

736493

-

-

?

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides

Bos taurus

-

acceptors are tri- or disaccharides with terminal glucuronyl residue and a galactosyl residue in penultimate position, transfers acetylgalactosamine in beta-linkage, no substrates are glucuronic acid or disaccharides with terminal galactosyl residues

638344

-

638344

?

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides

Homo sapiens

Q8TDX6

-

638345

-

638345

?

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides

Homo sapiens

Q8TDX6

-

638346

-

638346

?

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides

Homo sapiens

Q8N6G5, Q8TDX6

-

638347

-

638347

?

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides

Bos taurus

-

involved in chondroitin sulfate biosynthesis: transfer of the first N-acetylgalactosamine residue

638344

-

-

?

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides

Bos taurus

-

involved in the biosynthesis of chodroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core.

638344

-

-

?

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides

Homo sapiens

Q8TDX6

involved in chondroitin initiation and elongation

638345

-

-

?

UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides

UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides

Homo sapiens

Q8TDX6

involved in the biosynthesis of chodroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core.

638345

-

-

?

additional information

?

-

Homo sapiens

-

GalNAcT activity of the recombinant enzyme is determined using a trisaccharide analogue of the linkage region linked to a chromophoric aglycone, GlcA-beta1,3-Gal-beta1,3-Gal-O-methoxyphenyl, i.e. GlcA-Gal-Gal-OMP, as a acceptor substrate, Km is 3.8 mM, and with only the trisaccharide GlcA-beta1,3-Gal-beta1,3-Gal, Km 3.39 mM, no activity with Gal-Gal-OMP. The enzyme activity is even higher with 4- and 6-sulfated GlcA-Gal-Gal-OMP, kM values are 0.6 mM and 1.0 mm, respectively, no activity with 6-disulfated GlcA-Gal-Gal-OMP

722363

-

-

?

additional information

?

-

Mus musculus

-

in vitro chondroitin polymerization does not occur on the non-reducing terminal GalNAc-linkage pentasaccharide structure

721534

-

-

?

additional information

?

-

Mus musculus

Q8BJQ9

no activity with GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin and GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

736493

-

-

?

additional information

?

-

Mus musculus

Q8C1F4

no activity with GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin and GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

736493

-

-

?

additional information

?

-

Mus musculus C57BL/6

Q8BJQ9

no activity with GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin and GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

736493

-

-

?

additional information

?

-

Mus musculus C57BL/6

Q8C1F4

no activity with GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin and GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)

736493

-

-

?