2.4.1.173: sterol 3beta-glucosyltransferase

This is an abbreviated version!
For detailed information about sterol 3beta-glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.173

2.4.1.173

glucoside

steryl

glucosylation

pexophagy

somnifera

withania

glucosyltransferases

withanolide

stigmasterol

synthesis

sitosterol

3beta-hydroxy

orbiculare

udp-glucose-dependent

udpase

withaferin

Reaction

Synonyms

Atg26, AtSGT, autophagy-related protein 26, CG synthetase, pUGT51, ScAtg26, SGT, SGT1, SGT2, SGT3, SGT4, SGTL1, SgtL2, SgtL4, SlSGT1, SlSGT2, SlSGT3, SlSGT4, Solyc04g051150.2.1, Solyc04g071540.2.1, Solyc06g007980.2.1, Solyc09g061860.2.1, sterol glucosyltransferase, sterol glucosyltransferase Ugt51/Paz4, sterol glycosyltransferase, sterol-beta-D-glucosyltransferase, sterol:UDPG glucosyltransferase, UDP-Glc:sterol glucosyltransferase 80B1, UDP-glucose sterol glucosyltransferase, UDP-glucose sterol glycosyltransferase, UDP-glucose-sterol beta-glucosyltransferase, UDP-glucose-sterol glucosyltransferase, UDP-glucose:sterol glucosyltransferase, UDP-glycosyltransferase 51, UDPG-SGTase, UDPG:sterol glucosyltransferase, UDPglucose:sterol glucosyltransferase, Ugt51, Ugt51/Paz4, ugt51E1, UGT713B1, UGT80A2, UGT80B1, uridine diphosphoglucose-poriferasterol glucosyltransferase, uridine diphosphoglucose-sterol glucosyltransferase, USGT80B1

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.173 sterol 3beta-glucosyltransferase

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Results	in table
48	Activating Compound
1416	AA Sequence
4	Application
2	CAS Registry Number
31	Cloned(Commentary)
1	Crystallization (Commentary)
5	Disease/ Diagnostics
5	Expression
47	General Information
8	IC50 Value
71	Inhibitors
3	kcat/KM [mM/s]
2	Ki Value [mM]
51	KM Value [mM]
53	Localization
32	Metals/Ions
15	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
99	Organism
2	Pathway
4	PDB ID
16	pH Optimum
2	pH Range
23	pH Stability
1	pI Value
22	Protein Variants
25	Purification (Commentary)
1	Reaction
1	Reaction Type
73	Reference
68	Source Tissue
3	Specific Activity [micromol/min/mg]
3	Storage Stability
268	Substrates and Products (Substrate)
10	Subunits
110	Synonyms
1	Systematic Name
31	Temperature Optimum [°C]
1	Temperature Range [°C]
11	Turnover Number [1/s]

Purification

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Purification on EC 2.4.1.173 - sterol 3beta-glucosyltransferase

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Cibacron Blue column, Sephadex G-25 column and Mono Q anion exchange column

Withania somnifera

672427

gel filtration and ion exchange column chromatography

partial

partial purification by DEAE-cellulose column DE-52

Zea mays

637781

partial purification by differential centrifugation

2 entries

partial purification by differential centrifugation and DEAE-cellulose column

2 entries

partial purification by differential centrifugation and gel filtration on Sephadex G-100

Physarum polycephalum

637766, 637767

partial purification by differential centrifugation and precipitation with Me2CO

Sinapis alba

208429

partial purification by differential centrifugation and Sephadex G-100 column, DEAE-Sephadex A-50 and Sepharose6B

Digitalis purpurea

637770

partial purification by differential centrifugation and Sephadex G-150 column

Sinapis alba

637771

partially purified by gel filtration chromatography, resutlts in 3.73fold purification

Withania somnifera

Q2I015

671624

purification about 12500fold by microsomes, extraction with diethyl ether, Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE

Avena sativa

637782

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)

6 entries

recombinant N-terminally His6-tagged UGT51 residues 722-1198 Escherichia coli srain BL21(DE3) CodonPlus by nickel affinity chromatography, tag cleavage by thrombin, followed by a secons step of nickel affinity chromatography, gel filtration, and ultrafiltration

Saccharomyces cerevisiae

Q06321

759680