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2-Hydroxy-5-nitrobenzyl bromide
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2-mercaptoethanol
slight inhibition of the isozyme
4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid
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4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid
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4-thio-UDP
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50% of inhibition at 160 uM
ATP
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inhibition at 1-3 mM but slightly stimulation at low concentration
cetyltrimethylammonium bromide
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strong inhibition
cholesteryl glucoside
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diacylglycerol
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with saturated fatty acids
diethyldicarbonate
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protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose
GDP-glucose
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20% of inhibition at high concentration
GTP
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the inhibitory effect of GTP is less prominent as compared to UDP
Hg2+
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strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.5
iodoacetamide
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strong and irreversible inhibition by thiol directed reagents like 4-chloromercuribenzoate, N-methylmaleimide and iodoacetamide suggest the requirement of free-SH groups for the catalytic activity
maleate buffer
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strong inhibition at 50 mM
Mersalyl
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50% of inhibition at 40 uM
monoacylglycerol
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with saturated fatty acids
n-octyl thioglucoside
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irreversible inhibited, 50% of activity at 4.8 mM
Ni2+
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strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 1.2
p-chloromercuribenzenesulfonic acid
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p-mercuribenzenesulfonate
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50% of irreversible inhibition at 0.5 mM, UDP and UDPG-glucose protect from inhibition of 10 mM
Phenylglyoxal
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92% of inhibition at 10 mM
phosphate buffer
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strong inhibition at 50 mM
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pyridoxal 5'-phosphate
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triacylglycerol
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with saturated fatty acids
Triton
strong inhibition of isozyme GhSGT1
Tween 20
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strong inhibition at concentrations below 0.05%
Tween 60
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strong inhibition at concentrations below 0.05%
UDP-2',3'-dialdehyde
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inhibits irreversibly the enzyme
UDP-mannose
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50% of inhibition at 40 uM
Co2+
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Co2+
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strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 1.3
coprostanol
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potent inhibitor
coprostanol
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not inhibitory
epicholestanol
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potent inhibitor
epicholestanol
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not inhibitory
N-ethylmaleimide
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protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose
N-ethylmaleimide
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strong and irreversible inhibition by thiol directed reagents like 4-chloromercuribenzoate, N-methylmaleimide and iodoacetamide suggest the requirement of free-SH groups for the catalytic activity
p-chloromercuribenzoate
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potent inhibitory effect at 0.1 mM indicating that free SH groups are essential for activity
p-chloromercuribenzoate
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50% inhibition at 0.60 mM
p-chloromercuribenzoate
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strong and irreversible inhibition by thiol directed reagents like 4-chloromercuribenzoate, N-methylmaleimide and iodoacetamide suggest the requirement of free-SH groups for the catalytic activity
sodium deoxycholate
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strong inhibition at 0.2-0.8%
sodium deoxycholate
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strong inhibition at 0.1-1%
sodium taurocholate
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strong inhibition at 0.1-1%
Triton X-100
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strong inhibition at concentrations below 0.05%
Triton X-100
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moderate inhibition but strong inhibition at low concentrations
Triton X-100
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at higher levels (0.3%), Triton X-100 inactivats the enzyme, giving 10% of the original activity after 30 min pre-incubation
UDP
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total inhibition at 1 mM
UDP
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50% of inhibition at 70 uM
UDP
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50% inhibition at 0.06 mM
UDP
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the inhibitory effect of UTP and GTP is less prominent as compared to UDP
UMP
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inhibition at 1 mM
UTP
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total inhibition at 1 mM
UTP
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50% inhibition at 0.70 mM
UTP
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the inhibitory effect of UTP is less prominent as compared to UDP
Zn2+
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Zn2+
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strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.04
additional information
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not inhibited by ADP, GDP or inorganic phosphorus
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additional information
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inhibited at high ionic strength, 50% inhibition at 0.52 M NaF or 0.29 M NaCl; inhibited by dicarboxylic amino acids-modifying reagent Woodwards Reagent K protected against inactivation upon pre-incubation with UDP-glucose but not with stigmast-5-en-3beta-ol; no effect with Mg2+, Ca2+, Mn2+, Tween20, Tween 40, Tween 60, Tween 80, Tyloxapol, sodium deoxycholate, EDTA, EGTA, and reagents modifying serine, arginine and tyrosine side chains
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additional information
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not affected by tuber slicing or ethephon
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