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2.4.1.173: sterol 3beta-glucosyltransferase

This is an abbreviated version!
For detailed information about sterol 3beta-glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.173

Reaction

UDP-glucose
+
a sterol
=
UDP
 +
a sterol 3-beta-D-glucoside

Synonyms

Atg26, AtSGT, autophagy-related protein 26, CG synthetase, pUGT51, ScAtg26, SGT, SGT1, SGT2, SGT3, SGT4, SGTL1, SgtL2, SgtL4, SlSGT1, SlSGT2, SlSGT3, SlSGT4, Solyc04g051150.2.1, Solyc04g071540.2.1, Solyc06g007980.2.1, Solyc09g061860.2.1, sterol glucosyltransferase, sterol glucosyltransferase Ugt51/Paz4, sterol glycosyltransferase, sterol-beta-D-glucosyltransferase, sterol:UDPG glucosyltransferase, UDP-Glc:sterol glucosyltransferase 80B1, UDP-glucose sterol glucosyltransferase, UDP-glucose sterol glycosyltransferase, UDP-glucose-sterol beta-glucosyltransferase, UDP-glucose-sterol glucosyltransferase, UDP-glucose:sterol glucosyltransferase, UDP-glycosyltransferase 51, UDPG-SGTase, UDPG:sterol glucosyltransferase, UDPglucose:sterol glucosyltransferase, Ugt51, Ugt51/Paz4, ugt51E1, UGT713B1, UGT80A2, UGT80B1, uridine diphosphoglucose-poriferasterol glucosyltransferase, uridine diphosphoglucose-sterol glucosyltransferase, USGT80B1

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.173 sterol 3beta-glucosyltransferase

Cloned

Cloned on EC 2.4.1.173 - sterol 3beta-glucosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain DH5alpha
-
expression in Escherichia coli
expression in Escherichia coli strain XJb
gene AGT26, recombinant expression of N-terminally His6-tagged UGT51 residues 722-1198 from pET28a vector with a thrombin cleavage site in Escherichia coli srain BL21(DE3) CodonPlus
gene At1g43620, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern
gene At3g07020, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern. UGT80A2 expressing cells displays a greater than 100fold increase in cholesteryl glucoside production compared with the negative control
gene At5g24750, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression patterns. UGT713B1 displays little or no activity
gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene GhSGT1, DNA and amino acid sequence determination and analysis, the expression level of gene GhSGT1 is almost 10fold higher than that of gene GhSGT2
gene GhSGT2, DNA and amino acid sequence determination and analysis, the expression level of gene GhSGT1 is almost 10fold higher than that of gene GhSGT2
gene PGUG_04770, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene SGT1, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1
gene SGT2, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1
gene SGT3, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1
gene SGT4, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1
gene UGT80A2, quantitative reverse-transcription PCR expression analysis
gene UGT80B1, quantitative reverse-transcription PCR expression analysis
gene UGT80B1, recombinant expression of native promoter and 35 S driven GFP-tagged wild-type and mutant enzymes, the ugt80B1 mutant phenotype is complemented with pro35S:UGT80B1:GFP or with proUGT80B1:UGT80B1:GFP as observed by rescue of the transparent testa phenotype
gene WsSGTL1, ectopic overexpression in Withania somnifera using Agrobacterium tumefaciens transfection method, quantitative real-time PCR enzyme expression analysis. Overexpression of WsSGTL1 gene which is responsible for increase in glycosylated withanolide and sterols, and confers better growth and tolerance to both biotic and abiotic stresses
-
protein encoded by ugt51E1 is expressed in Escherichia coli
-
the open reading frame of SGTL1 is expressed in Escherichia coli strain DH5alpha