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2.4.1.16: chitin synthase

This is an abbreviated version!
For detailed information about chitin synthase, go to the full flat file.

Word Map on EC 2.4.1.16

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
[(1->4)-N-acetyl-beta-D-glucosaminyl]n
=
UDP
 +
[(1->4)-N-acetyl-beta-D-glucosaminyl]n+1

Synonyms

AaCHS-1, acetylglucosaminyltransferase, chitin-uridine diphosphate, AgCHS-1, AgCHS-2, Ar-CS1, BcChsVI, BmCHSA-2a, BmCHSA-2b, CaChs1, CaChs2p, chitin synthase 1, chitin synthase 2, chitin synthase 3, chitin synthase 3a, chitin synthase 4, chitin synthase 5, chitin synthase 6, chitin synthase 7, chitin synthase A, chitin synthase B, chitin synthase II, chitin synthase III, chitin synthase protein 2, chitin synthetase, chitin synthetase I, chitin synthetase II, chitin synthetase III, chitin-UDP acetyl-glucosaminyl transferase 1, chitin-UDP acetyl-glucosaminyl transferase 2, chitin-UDP acetyl-glucosaminyl transferase 3, chitin-UDP acetyl-glucosaminyl transferase 4, chitin-UDP acetyl-glucosaminyl transferase 5, chitin-UDP acetyl-glucosaminyl transferase 6, chitin-UDP acetyl-glucosaminyl transferase 7, chitin-UDP N-acetylglucosaminyltransferase, chitin-uridine diphosphate acetylglucosaminyltransferase, CHS, CHS B, chs-1, chs-2, CHS-3, CHS-6, Chs1, CHS1a, CHS1b, CHS1p, Chs2, CHS2p, CHS3, CHS3a, Chs3p, CHS4, Chs4p, CHS5, CHS6, CHS7, Chs8, chsA, chsB, chsC, chsE, CHSI, CHSIIIa, CHSIIIb, class A chitin synthase, class I CHS, class IV CaChs3p, class IV chitin synthase, class IV CHS, class V chitin synthase, class Vb chitin synthase, class VII chitin synthase, class-A CHS, class-B CHS, class-II chitin synthase 1, CSI, CSII, CSIII, CsmA, CsmB, DmCHS-1, DmCHS-2, DmeChSB, EhCHS-1, EhCHS-2, LcCHS-1, LeChs1, LsCHS-1, Mcs1, More, MsCHS-1, MsCHS1, MsCHS2, myosin motor-like chitin synthase, Ov-chs-2, PdChsVII, TcCHS1, TcCHS2, trans-N-acetylglucosaminosylase, UDP-GlcNAc:chitin 4-alpha-N-acetylglucosaminyltransferase, UmCHS3, UmCHS6, WdCHS1, WdChs3p, WdChs5p

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.16 chitin synthase

Cloned

Cloned on EC 2.4.1.16 - chitin synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chitin synthase 2
-
cloning and expression of class I CHS isozyme
cloning and expression of class III CHS isozyme
cloning and expression of class IV and V CHS isozymes
cloning from anterior midguts of fifth instar larvae, expression in Saccharomyces cerevisiae AH109 cells and protein interaction analysis by yeast two hybrid screening, identifying a chymotrypsin-like protease CTLP1 that binds to the extracellular carboxyterminal domain of CHS2
comparative sequence analysis, cloning and expression of the enzyme's transmembrane segment ArCS1_E22TM, recombinant expression of the His-tagged domain in Dictyostelium discoideum, the corresponding transmembrane protein ArCS1_E22TM accumulates in membrane fractions of the expression host Dictyostelium discoideum, recombinant expression of clone EIIIab containing the ArCS1_E22 cDNA fragment in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Saccharomyces cerevisiae using the GAL1 promoter in a multicopy plasmid
-
expression in CF203/2.5 insect cell
expression in Escherichia coli
expression of a 12 kDa Myo12p epitope located in the C-terminal end of the N-terminal myosin motorlike domain with a P-loop and use for generation of antibodies
expression of Chs4p-GFP and Chs3p-GFP in CRM233 and CRM103 strains, respectively
functional expression in Pichia pastoris
gene BcChs6, cloning in Escherichia coli strain DH5alpha, Agrobacterium-mediated transformation of Bortrytis cinerea spores for gene disruption
gene BmChsA, semiquantitative RT-PCR expression analysis, expression pattern
gene BmChsB, DNA and amino acid sequence determination and analysis, seqeunce comparisons, quantitative expression analysis and expression profile
gene chs-3, DNA and amino acid sequence determination and analysis, expression of GFP-labeled CHS-3 in hyphae and subcellular localization, overview
gene chs-6, DNA and amino acid sequence determination and analysis, expression of GFP-labeled CHS-6 in hyphae and subcellular localization, overview
gene CHS1, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview
gene chs2, DNA and amino acid sequence determination and analysis
gene CHS2, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview
gene CHS3, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview
gene CHS8, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview
gene CHSA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of a fragment comprising base pairs 2040-2495 as His-tagged protein
gene CHSA, expression analysis
gene CHSB, DNA and amino acid sequence determination and analysis, genetic structure,phylogenetic analysis, expression analysis, sequence comparisons
gene chsVII, phylogenetic analysis, quantitative RT-PCR enzyme expression analyis
gene Lechs1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genetic structure, expression in Escherichia coli strain JM109
gene Pochs1, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative expression analysis
-
genes CHS1-4, phylogenetic tree, expression of deeltion mutants, quantitative expression analysis by RT-PCR
-
high level expression of wild-type Chs2 and a mutant Chs2DELTAN222 lacking the N-terminal region in Escherichia coli strain C41 and in Pichia pastoris strain SMD1163 membranes in an active form, the latter system results in higher enzyme activity, overview
-
myosin motor-like chitin synthase gene chsVb, DNA and amino acid sequence determination and analysis, expression analysis, phylogenetic tree and analysis
N-terminally deleted enzyme is expressed in Escherichia coli BL21 Star (DE3) cells
-
overexpression in Saccharomyces cerevisiae
-
overexpression of CHS isozymes in Saccharomyces cerevisiae strains
-
overexpression of CHS2p isozyme in Saccharomyces cerevisiae strains
-
since chitin synthase 2 can not expressed in bacterial cells or insect cells in an active form, Saccharomyces cerevisiae is used as the host for overexpression
-
the entire region of csmB cDNA is cloned by 5' and 3' RACE methods
WdChs5p-myc protein has a differential expression feature that is similar to the differential transcription of the WdCHS5 gene, transcriptional regulation is the first and probably the most important control point of the expression ofWdCHS5, overview, expression of wild-type and myc-tagged enzyme in Escherichia coli strain DH5alpha, expression of WdChs5p-myc protein in Wangiella dermatitidis, expression patterns, overview