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2.4.1.15: alpha,alpha-trehalose-phosphate synthase (UDP-forming)

This is an abbreviated version!
For detailed information about alpha,alpha-trehalose-phosphate synthase (UDP-forming), go to the full flat file.

Word Map on EC 2.4.1.15

Reaction

UDP-alpha-D-glucose
+
D-glucose 6-phosphate
=
UDP
 +
alpha,alpha-trehalose 6-phosphate

Synonyms

alpha,alpha-trehalose phosphate synthase (UDP-forming), alpha,alpha-trehalose-phosphate synthase (UDP-forming), AtTPS1, AtTPS6, GbTPS, glucosyltransferase, uridine diphosphoglucose phosphate, OtsA, OtsA1, OtsA2, phosphotrehalose-uridine diphosphate transglucosylase, T-6-P, T6P synthase, Ta1210, TPS, TPS-2, TPS-3, TPS1, TPS2, TPS3, TPS5, TPSP, transglucosylase, trehalose 6-phosphate synthase, trehalose 6-phosphate synthase 1, trehalose 6-phosphate synthetase, trehalose phosphate synthase, trehalose phosphate synthase 5, trehalose phosphate synthetase, trehalose phosphate-uridine diphosphate glucosyltransferase, trehalose-6-P synthase, trehalose-6-phosphate synthase, trehalose-6-phosphate synthase 1, trehalose-6-phosphate synthase/phosphatase, trehalose-6P synthase, trehalose-P synthetase, trehalosephosphate-UDP glucosyl transferase, UDP-glucose:D-glucose-6-phosphate 1-alpha-D-glucosyltransferase, UDPglucose-glucose-phosphate glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.15 alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Purification

Purification on EC 2.4.1.15 - alpha,alpha-trehalose-phosphate synthase (UDP-forming)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Arabidopsis thaliana extracts are prepared, AtTPS1 expressed in yeast is analysed in yeast extracts
-
bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase
-
crude extract is prepared, the enzyme is isolated by ammonium sulfate fractionation and high performance gel permeation liquid chromatography, using a HiLoad 16/60 Superdex 200 and a Ultropak TSK G2000SW column
-
glutathione-S-transferase-Tps1p fusion protein expressed in E. coli
-
heparin helps to retain both stability and activity of the final purified enzyme
-
native enzyme 22.1fold to homogeneity by several gel filtration steps
-
native enzyme 50fold by ammonium sulfate fractionation, gel filtration, and nickel-resin affinity chromatography
-
native enzyme from female worm muscle 265fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
Ni-NTA agarose column chromatography
Ni-NTA column chromatography and Superdex S200 gel filtration
nickel chelating bead chromatography and Superdex S200 gel filtration
partial
recombinant enzyme from Escherichia coli
recombinant His-tagged TPS1 from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain B21(DE3) by nickel affinity chromatography
recombinant His10-tagged isozyme from Escherichia coli strain BL21 CodonPlus(DE3)-RP by nickel affinity chromatography, ultrafiltration, tag cleavage by Factor Xa
recombinant soluble MalE-CfTPS fusion protein from Escherichia coli Rosetta (DE3) cells partially by by amylose-agarose affinity chromatography
separation of three aggregates of trehalose 6-phosphate synthase (molecular weights of 624000 Da, 224000 Da and 107000 Da) by gel filtration and ion exchange chromatography
-
trehalose-6-phosphate synthase/phosphatase complex, EC 2.4.1.15/EC 3.1.3.12
-