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2.4.1.15: alpha,alpha-trehalose-phosphate synthase (UDP-forming)

This is an abbreviated version!
For detailed information about alpha,alpha-trehalose-phosphate synthase (UDP-forming), go to the full flat file.

Word Map on EC 2.4.1.15

Reaction

UDP-alpha-D-glucose
+
D-glucose 6-phosphate
=
UDP
 +
alpha,alpha-trehalose 6-phosphate

Synonyms

alpha,alpha-trehalose phosphate synthase (UDP-forming), alpha,alpha-trehalose-phosphate synthase (UDP-forming), AtTPS1, AtTPS6, GbTPS, glucosyltransferase, uridine diphosphoglucose phosphate, OtsA, OtsA1, OtsA2, phosphotrehalose-uridine diphosphate transglucosylase, T-6-P, T6P synthase, Ta1210, TPS, TPS-2, TPS-3, TPS1, TPS2, TPS3, TPS5, TPSP, transglucosylase, trehalose 6-phosphate synthase, trehalose 6-phosphate synthase 1, trehalose 6-phosphate synthetase, trehalose phosphate synthase, trehalose phosphate synthase 5, trehalose phosphate synthetase, trehalose phosphate-uridine diphosphate glucosyltransferase, trehalose-6-P synthase, trehalose-6-phosphate synthase, trehalose-6-phosphate synthase 1, trehalose-6-phosphate synthase/phosphatase, trehalose-6P synthase, trehalose-P synthetase, trehalosephosphate-UDP glucosyl transferase, UDP-glucose:D-glucose-6-phosphate 1-alpha-D-glucosyltransferase, UDPglucose-glucose-phosphate glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.15 alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Cloned

Cloned on EC 2.4.1.15 - alpha,alpha-trehalose-phosphate synthase (UDP-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
11 TPS genes, class I AtTPS1-AtTPS4 and class II AtTPS5-AtTPS11. Expression of genes AtTPS2 or AtTPS4 enables the yeast tps1DELTA tps2DELTA mutant to grow on glucose and accumulate trehalose 6-phosphate and trehalose. Class II TPS genes do not complement the yeast mutant
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a translational trehalose-6-phosphate synthase - trehalose-6-phosphate phosphatase, TPS-TPP, gene-fusion is constructed and subcloned into the pBluescript SK vector, into the yeast expression vector pSal4, and into the pBin19 vector for transformation of Arabidopsis thaliana
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Agrobacterium tumefaciens-mediated transformation of Nicotiana tabacum. The enzyme is markedly present in the vacuoles and the cell wall, and to a lesser extent in the cytosol
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construction of a bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase and expression in Escherichia coli
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construction of a new transformation vector containing the AtTPS1 gene as a selection marker. Overexpression of AtTPS1 in tobacco allows selecting glucose insensitive transgenic shoots
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DNA and amino acid sequence determination and analysis, overexpression in transgenic flies, functional expression in HEK-293 cells
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expressed in Escherichia coli B834(DE3) cells
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli Rosetta-gami 2(DE3) cells
expression in Escherichia coli
expression in Pichia pastoris
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expression of glutathione-S-transferase-Tps1p fusion protein in Escherichia coli
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expression of the TPS1 gene in transgenic Solanum tuberosum, usage of potato wild-type drought-sensitive cultivar White Lady
expression of TPS1 driven by the ABI3 promoter rescues the severe embryo phenotype but not the vegetative phenotype of tps1
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functional expression of the enzyme as a fusion protein with Escherichia coli trehalose-6-phosphate phosphatase in transgenic Oryza sativa plants
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functional expression of the enzyme as a fusion protein with Escherichia coli trehalose-6-phosphate phosphatase in transgenic Oryza sativa plants, expression in human fibroblasts
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gene cftps, DNA and amino acid sequence determination and anaysis, phylogenetic analysis, expression of largely soluble MalE-CfTPS fusion protein in Escherichia coli Rosetta (DE3) cells
gene dmtps1, phylgenetic analysis, expression of His-tagged TPS1 in Escherichia coli strain BL21
gene otsA, cloned from genetic library, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21
gene otsA, DNA amnd amino acid sequence determination and analysis
gene otsA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B21(DE3)
gene otsA1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, cloning in Escherichia coli strain DH5alpha, recombinant expression as His10-tagged isozyme in Escherichia coli strain BL21 CodonPlus(DE3)-RP
gene otsA2, DNA and amino acid sequence determination and analysis, , phylogenetic analysis, cloning in Escherichia coli strain DH5alpha, recombinant expression as His10-tagged isozyme in Escherichia coli strain BL21 CodonPlus(DE3)-RP
gene RoTPS1, DNA and amino acid sequence determination and analysis, sequence comparisons, cloning in Escherichia coli strain DH5alpha, recombinant expression in Saccharomyces cerevisiae strain YSH 290 and secretion of the recombinant enzyme
gene TA1210, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strains DH5alpha and BL21-CodonPlus (DE3)-RIL
gene tps, a single copy, genetic structure, DNA and amino acid sequence determination and analysis, wild-type realtime PCR expression and promoter region analysis, expression in Saccharomyces cerevisiae tps1DELTA mutant YSH290 strain leading to no functional complementation
gene tps, four copies, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative reverse transcriptase PCR enzyme expression analysis and profile
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gene tps1, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene TPS1, Saccharomyces cerevisiae strain 10151 is used as host strain for transformation and overexpression, strains EP-1 and 14801 are used for preparing chromosomal DNAs
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gene tps2, DNA and amino acid sequence determination and analysis, phylogenetic analysis
into the pDONOR207 vector and after confirming the sequence subcloned into the pBIN-GW binary vector, into the expression vector pYES2, for transformation of yeast cells
isozyme TPS1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crabl. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis
isozyme TPS1a, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis
isozyme TPS1b, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis
isozyme TPS1c, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis
overexpression in Arabidopsis thaliana plants under control of the 35S promotor
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overexpression in the Bombyx mori baculovirus expression system
overexpression of the enzyme incresases stress resistance of yeast cells
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pET-based system in BL21 cells
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preparation of a recombinant strain carrying multiple copies of ggsA by cotransformation
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the shuttle vectors pSAL4 and pSAL6, the YEplac195/KanMX plasmid, and the two-hybrid plasmids pGBT9 and pGAD242 are used, GFP-fusions are generated using pK7GWF2 and pHGWR2
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the TPS1 gene of Saccharomyces cerevisiae is cloned into pBluescript II SK, in addition a binary vector for transformation of potato plants is constructed
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transgenic Arabidopsis plants with T6P levels increased by expression of T6P synthase or decreased by expression of T6P phosphatase in the cytosol. Compared with wild type, leaves of T6P synthase-expressing plants have increased redox activation of ADP-glucose pyrophosphorylase and increased starch
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