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2.4.1.140: alternansucrase

This is an abbreviated version!
For detailed information about alternansucrase, go to the full flat file.

Word Map on EC 2.4.1.140

Reaction

[Glc(alpha1-6)Glcalpha(1-3)]n
+ 2 sucrose =
[Glc(alpha1-6)Glcalpha(1-3)]n+2
+ 2 beta-D-fructofuranose

Synonyms

alternansucrase, ASR, glucansucrase, glucosyltransferase, sucrose-1,6(3)-alpha-glucan 6(3)-alpha-, Lcalt, sucrose:1,6-, 1,3-alpha-D-glucan 3-alpha- and 6-alpha-D-glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.140 alternansucrase

Purification

Purification on EC 2.4.1.140 - alternansucrase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
rcombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and dialysis
recombinant wild-type enzyme and truncated mutants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by ultrafiltration