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2.7
(1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
pH 6.2, 37°C, recombinant enzyme
152
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
1.6
2-naphthyl 1,5-dithio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
2
2-naphthyl 5-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
30.2
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
47.2
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
41
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.13 - 4080
4-methylumbelliferyl-beta-D-xylopyranoside
5460
4-nitrophenyl-beta-D-xylopyranoside
recombinant maltose-binding protein-beta1,4-GalT7 fusion protein
78.7
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
64.3
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
2.7
naphthalen-2-yl 1-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
3.1
naphthalen-2-yl beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
106.3
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.5 - 1.93
UDP-alpha-D-galactose
0.028 - 5086
UDP-galactose
72
UDP-glucose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
3 - 6
UDP-xylose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
0.13
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
0.5
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y196F
0.77
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270A
0.81
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270K
0.89
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226A
1.21
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y199F
1.35
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226K
1.49
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195R
1.5
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant wild-type enzyme
1.62
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195Q
1.79
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
1.93
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195A
4080
4-methylumbelliferyl-beta-D-xylopyranoside
recombinant maltose-binding protein-beta1,4-GalT7 fusion protein
0.5
UDP-alpha-D-galactose
mutant enzyme Y196F, at pH 7.0 and 37°C
0.5
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y196F
0.77
UDP-alpha-D-galactose
mutant enzyme R270A, at pH 7.0 and 37°C
0.77
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270A
0.81
UDP-alpha-D-galactose
mutant enzyme R270K, at pH 7.0 and 37°C
0.81
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270K
0.89
UDP-alpha-D-galactose
mutant enzyme R226A, at pH 7.0 and 37°C
0.89
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226A
1.21
UDP-alpha-D-galactose
mutant enzyme Y199F, at pH 7.0 and 37°C
1.21
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y199F
1.35
UDP-alpha-D-galactose
mutant enzyme R226K, at pH 7.0 and 37°C
1.35
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226K
1.49
UDP-alpha-D-galactose
mutant enzyme H195R, at pH 7.0 and 37°C
1.49
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195R
1.5
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant wild-type enzyme
1.51
UDP-alpha-D-galactose
wild type enzyme, at pH 7.0 and 37°C
1.62
UDP-alpha-D-galactose
mutant enzyme H195Q, at pH 7.0 and 37°C
1.62
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195Q
1.93
UDP-alpha-D-galactose
mutant enzyme H195A, at pH 7.0 and 37°C
1.93
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195A
0.028
UDP-galactose
-
wild-type, pH and temperature not specified in the publication
0.1
UDP-galactose
pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli
0.46
UDP-galactose
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
1
UDP-galactose
-
DELTA1-81, mutant A186D, pH and temperature not specified in the publication
1
UDP-galactose
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.05
UDP-galactose
-
DELTA1-81, mutant R270C, pH and temperature not specified in the publication
1.05
UDP-galactose
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.06
UDP-galactose
-
DELTA1-81, mutant L206P, pH and temperature not specified in the publication
1.06
UDP-galactose
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.11
UDP-galactose
-
DELTA1-81, wild-type, pH and temperature not specified in the publication
1.11
UDP-galactose
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.14
UDP-galactose
-
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C
1.52
UDP-galactose
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
5086
UDP-galactose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
-
kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-mannose
kcat value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-mannose
-
kcat value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl galactosamine
kcat value not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl galactosamine
-
kcat value not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl glucosamine
kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl glucosamine
-
kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7