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0.39
(1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
pH 6.2, 37°C, recombinant enzyme
0.58
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.025
2-naphthyl 1,5-dithio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.034
2-naphthyl 5-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.75
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.53
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.75
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.35 - 1.06
4-methylumbelliferyl alpha-D-xylopyranoside
0.27 - 4.22
4-methylumbelliferyl O-beta-D-xylopyranoside
0.16 - 4.22
4-methylumbelliferyl-beta-D-xylopyranoside
0.33 - 0.5
4-methylumbelliferyl-beta-D-xyloside
1.27 - 7.93
4-nitrophenyl O-beta-D-xylopyranoside
0.85 - 7.93
4-nitrophenyl-beta-D-xylopyranoside
1.41
beta-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2)-alpha-D-mannosyl-1,6-beta-D-mannosyl-octyl
-
pH 7, 37°C
0.91
N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-beta-D-mannosyl-octyl
-
pH 7, 37°C
2.29
N-acetyl-beta-D-glucosaminyl-1,6-(beta-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,2)-alpha-D-mannosyl-1,6-beta-D-mannosyl-octyl
-
pH 7, 37°C
0.33
N-acetyl-beta-D-glucosaminyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2)-alpha-D-mannosyl-1,6-beta-D-mannosyl-octyl
-
pH 7, 37°C
0.88
N-acetyl-beta-D-glucosaminyl-1,6-alpha-D-mannosyl-1,6-beta-D-mannosyl-octyl
-
pH 7, 37°C
0.4
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.56
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.36
naphthalen-2-yl 1-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.78
naphthalen-2-yl beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.53
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
1.2
p-nitrophenyl beta-D-xylose
-
37°C
0.22 - 0.4
UDP-alpha-D-galactose
0.02 - 2.41
UDP-galactose
0.28
UDP-glucose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
0.13
UDP-xylose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
additional information
-
0.35
4-methylumbelliferyl alpha-D-xylopyranoside
wild type enzyme, at pH 7.0 and 37°C
0.35
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195R, at pH 7.0 and 37°C
0.44
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R226A, at pH 7.0 and 37°C
0.46
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R226K, at pH 7.0 and 37°C
0.54
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R270K, at pH 7.0 and 37°C
0.55
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195Q, at pH 7.0 and 37°C
0.59
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme Y199F, at pH 7.0 and 37°C
0.6
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R270A, at pH 7.0 and 37°C
0.64
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195A, at pH 7.0 and 37°C
1.06
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme Y196F, at pH 7.0 and 37°C
0.27
4-methylumbelliferyl O-beta-D-xylopyranoside
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.32
4-methylumbelliferyl O-beta-D-xylopyranoside
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.38
4-methylumbelliferyl O-beta-D-xylopyranoside
mutant enzyme F221Y, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.53
4-methylumbelliferyl O-beta-D-xylopyranoside
mutant enzyme V164A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.6
4-methylumbelliferyl O-beta-D-xylopyranoside
-
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C
1.76
4-methylumbelliferyl O-beta-D-xylopyranoside
mutant enzyme D165E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
2.26
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
2.99
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
3.26
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
4.22
4-methylumbelliferyl O-beta-D-xylopyranoside
mutant enzyme F221A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.16
4-methylumbelliferyl-beta-D-xylopyranoside
membrane beta1,4-GalT7 in HeLa cells
0.16
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant W222F, protein expressed in HeLa cells
0.27
4-methylumbelliferyl-beta-D-xylopyranoside
maltose-binding protein-beta1,4-GalT7 fusion protein
0.27
4-methylumbelliferyl-beta-D-xylopyranoside
-
DELTA1-81, wild-type, pH and temperature not specified in the publication
0.32
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, wild-type, protein expressed in HeLa cells
0.33
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
0.35
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant wild-type enzyme
0.35
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195R
0.38
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant F221Y, protein expressed in HeLa cells
0.44
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226A
0.46
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226K
0.52
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant E227D, protein expressed in HeLa cells
0.53
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant V164A, protein expressed in HeLa cells
0.54
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270K
0.55
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195Q
0.57
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant D230A, protein expressed in HeLa cells
0.59
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y199F
0.6
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270A
0.64
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195A
0.73
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant G223A, protein expressed in HeLa cells
0.74
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
1.06
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y196F
1.76
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant D165E, protein expressed in HeLa cells
2.26
4-methylumbelliferyl-beta-D-xylopyranoside
-
DELTA1-81, mutant A186D, pH and temperature not specified in the publication
2.57
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant D228E, protein expressed in HeLa cells
2.99
4-methylumbelliferyl-beta-D-xylopyranoside
-
DELTA1-81, mutant L206P, pH and temperature not specified in the publication
3.26
4-methylumbelliferyl-beta-D-xylopyranoside
-
DELTA1-81, mutant R270C, pH and temperature not specified in the publication
4
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant W224H, value above 4, protein expressed in HeLa cells
4.22
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant F221A, protein expressed in HeLa cells
0.33
4-methylumbelliferyl-beta-D-xyloside
-
pH 5.5, 37°C after preincubation with ATP
0.5
4-methylumbelliferyl-beta-D-xyloside
-
pH 5.5, 37°C before preincubation with ATP
1.27
4-nitrophenyl O-beta-D-xylopyranoside
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
4.25
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
5.54
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
7.93
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.85
4-nitrophenyl-beta-D-xylopyranoside
membrane beta1,4-GalT7 in HeLa cells
0.85
4-nitrophenyl-beta-D-xylopyranoside
-
wild-type, pH and temperature not specified in the publication
1.27
4-nitrophenyl-beta-D-xylopyranoside
maltose-binding protein-beta1,4-GalT7 fusion protein
1.27
4-nitrophenyl-beta-D-xylopyranoside
-
DELTA1-81, wild-type, pH and temperature not specified in the publication
2.29
4-nitrophenyl-beta-D-xylopyranoside
-
mutant L206A, pH and temperature not specified in the publication
3.76
4-nitrophenyl-beta-D-xylopyranoside
-
mutant A186D, pH and temperature not specified in the publication
4.25
4-nitrophenyl-beta-D-xylopyranoside
-
DELTA1-81, mutant A186D, pH and temperature not specified in the publication
5.01
4-nitrophenyl-beta-D-xylopyranoside
-
mutant R270C, pH and temperature not specified in the publication
5.54
4-nitrophenyl-beta-D-xylopyranoside
-
DELTA1-81, mutant L206P, pH and temperature not specified in the publication
7.93
4-nitrophenyl-beta-D-xylopyranoside
-
DELTA1-81, mutant R270C, pH and temperature not specified in the publication
0.22
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant wild-type enzyme
0.22
UDP-alpha-D-galactose
wild type enzyme, at pH 7.0 and 37°C
0.27
UDP-alpha-D-galactose
mutant enzyme R270A, at pH 7.0 and 37°C
0.27
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270A
0.29
UDP-alpha-D-galactose
mutant enzyme R226K, at pH 7.0 and 37°C
0.29
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226K
0.32
UDP-alpha-D-galactose
mutant enzyme H195R, at pH 7.0 and 37°C
0.32
UDP-alpha-D-galactose
mutant enzyme Y199F, at pH 7.0 and 37°C
0.32
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195R
0.32
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y199F
0.33
UDP-alpha-D-galactose
mutant enzyme H195Q, at pH 7.0 and 37°C
0.33
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195Q
0.34
UDP-alpha-D-galactose
mutant enzyme R226A, at pH 7.0 and 37°C
0.34
UDP-alpha-D-galactose
mutant enzyme Y196F, at pH 7.0 and 37°C
0.34
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226A
0.34
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y196F
0.37
UDP-alpha-D-galactose
mutant enzyme R270K, at pH 7.0 and 37°C
0.37
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270K
0.4
UDP-alpha-D-galactose
mutant enzyme H195A, at pH 7.0 and 37°C
0.4
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195A
0.02
UDP-galactose
pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli
0.07
UDP-galactose
pH 7.0, 37°C, mutant W224H, protein expressed in HeLa cells
0.07
UDP-galactose
mutant enzyme W224H, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.1
UDP-galactose
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
0.21
UDP-galactose
pH 7.0, 37°C, mutant W222A, protein expressed in HeLa cells
0.21
UDP-galactose
mutant enzyme W222A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.23
UDP-galactose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
0.23
UDP-galactose
-
DELTA1-81, wild-type, pH and temperature not specified in the publication
0.23
UDP-galactose
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.28
UDP-galactose
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
0.29
UDP-galactose
-
wild-type, pH and temperature not specified in the publication
0.31
UDP-galactose
membrane beta1,4-GalT7 in HeLa cells
0.31
UDP-galactose
-
mutant L206A, pH and temperature not specified in the publication
0.31
UDP-galactose
pH 7.0, 37°C, wild-type, protein expressed in HeLa cells
0.31
UDP-galactose
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.33
UDP-galactose
pH 7.0, 37°C, mutant F221Y, protein expressed in HeLa cells
0.33
UDP-galactose
mutant enzyme F221Y, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.36
UDP-galactose
pH 7.0, 37°C, mutant F221A, protein expressed in HeLa cells
0.36
UDP-galactose
mutant enzyme F221A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.43
UDP-galactose
pH 7.0, 37°C, mutant D230A, protein expressed in HeLa cells
0.43
UDP-galactose
mutant enzyme D230A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.45
UDP-galactose
pH 7.0, 37°C, mutant G223A, protein expressed in HeLa cells
0.45
UDP-galactose
pH 7.0, 37°C, mutant W222F, protein expressed in HeLa cells
0.45
UDP-galactose
mutant enzyme G223A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.45
UDP-galactose
mutant enzyme W222F, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.45
UDP-galactose
-
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C
0.47
UDP-galactose
pH 7.0, 37°C, mutant D228E, protein expressed in HeLa cells
0.47
UDP-galactose
pH 7.0, 37°C, mutant E227D, protein expressed in HeLa cells
0.47
UDP-galactose
mutant enzyme D228E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.47
UDP-galactose
mutant enzyme E227D, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.48
UDP-galactose
pH 7.0, 37°C, mutant V164A, protein expressed in HeLa cells
0.48
UDP-galactose
mutant enzyme V164A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.49
UDP-galactose
-
mutant R270C, pH and temperature not specified in the publication
0.63
UDP-galactose
-
DELTA1-81, mutant R270C, pH and temperature not specified in the publication
0.63
UDP-galactose
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.65
UDP-galactose
-
DELTA1-81, mutant L206P, pH and temperature not specified in the publication
0.65
UDP-galactose
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.17
UDP-galactose
pH 7.0, 37°C, mutant D165E, protein expressed in HeLa cells
1.17
UDP-galactose
mutant enzyme D165E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
2.07
UDP-galactose
-
DELTA1-81, mutant A186D, pH and temperature not specified in the publication
2.07
UDP-galactose
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
2.41
UDP-galactose
-
mutant A186D, pH and temperature not specified in the publication
0.05
UDPgalactose
-
37°C
0.05
UDPgalactose
-
pH 6.5, 30°C
0.34
UDPgalactose
-
pH 5.5, 37°C after preincubation with ATP
0.41
UDPgalactose
-
pH 5.5, 37°C before preincubation with ATP
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics, molecular docking simulations of the Michaelis complex
-
additional information
UDP-glucuronic acid
KM value is above 4 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
-
KM value is above 4 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-mannose
KM value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-mannose
-
KM value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl galactosamine
KM value is not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl galactosamine
-
KM value is not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl glucosamine
KM value is above 10 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl glucosamine
-
KM value is above 10 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7