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2.4.1.133: xylosylprotein 4-beta-galactosyltransferase

This is an abbreviated version!
For detailed information about xylosylprotein 4-beta-galactosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.133

Reaction

UDP-alpha-D-galactose
+
[protein]-3-O-(beta-D-xylosyl)-L-serine
=
UDP
 +
[protein]-3-O-(beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine

Synonyms

B4GALT7, beta-1,4 galactosyltransferase I, beta-1,4 galactosyltransferase V, beta-1,4-galactosyltransferase, beta-1,4-galactosyltransferase 7, beta-1,4-galactosyltransferase I, beta-1,4-galactosyltransferase-I, beta-1,4-galactosyltransferase-T7, beta-1,4-GalT, beta-1,4-GalT 7, beta-1,4-GalT I, beta-1,4-GalT V, beta-1,4-GalT-I, beta1,4-galactosyltransferase 7, beta1,4-galactosyltransferase I, beta1,4-galactosyltransferase VII, beta1,4-galactosyltransferase-7, beta1,4-GalT, beta1,4-GalT-I, beta1,4-GalT7, beta4-galactosyltransferase 7, beta4Gal-T7, beta4galactosyltransferase I, beta4GalT-7, beta4GalT7, dbeta4GalTI, galactosyltransferase I, galactosyltransferase, uridine diphosphogalactose-xylose, GalT I, GalT-I, hbeta4GalT7, MgGalT7, pNP-xylose-beta-1,4-galactosyltransferase, proteoglycan UDP-galactose:beta-xylose beta 1,4-galactosyltransferase I, SQV-3, UDP-D-galactose:D-xylose galactosyltransferase, UDP-D-galactose:xylose galactosyltransferase, UDP-galactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase, UDPgalactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase, xylosylprotein 4-beta-galactosyltransferase, xylosylprotein beta1,4-galactosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.133 xylosylprotein 4-beta-galactosyltransferase

Cloned

Cloned on EC 2.4.1.133 - xylosylprotein 4-beta-galactosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and expression of human membrane beta1,4-GalT7 in HeLa cells, expression of soluble form as a fusion protein with maltose-binding protein in Escherichia coli
expressed as a GST-fusion protein in Escherichia coli and as Myc-tagged fusion proteins in HeLa cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells, HeLa cells and CHO pgsB-618 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli Rosetta(DE3)pLysS cells
expressed in in CHO618 cells as membrane protein and in Escherichia coli as soluble protein fused to maltose-binding protein
-
expression in Cos-1 cells
-
expression in Escherichia coli
expression in galactosyltransferase-deficient CHO618 cells
-
expression in Pichia pastoris
expression in Spodoptera frugiperda Sf9 cells
-
expression in Spodoptera frugiperda SF9 cells as prot-A-beta4-GalT fusion protein
-
expression in transgenic mice
-
expression in Xenopus laevis oocytes
-
expression of a hybrid enzyme (called xylGalT), consisting of the N-terminal domain of Arabidopsis thaliana xylosyltransferase and the catalytic domain of human beta-1,4-galactosyltransferase I (GalT) in tobacco causes a sharp reduction of N-glycans with potentially immunogenic corebound xylose (Xyl) and fucose (Fuc) residues
gene B4GALT7, recombinant expression of GST-tagged wild-type and mutant enzymes in CHOpgsB-618 cells, recombinant expression of the soluble GST-tagged mutant enzyme in Escherichia coli strain BL21(DE3)
gene B4GALT7, recombinant expression of His6-GST-tagged enzyme
gene B4GALT7, recombinant expression of N-terminally His6-tagged enzyme
gene CGI_10013446, transcriptome screening and DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
mutated beta1,4-GalT7s are expressed as soluble maltose-binding protein fusions in which the N-terminal cytoplasmic domain, the transmembrane segment and the stem region, which precede the catalytic domain and correspond to amino acids 1-81, are deleted
-
quantitative enzyme expression analysis by RT-PCR
transient expression in mouse fibroblast L cells and chinese hamster ovary K-1 cells
-
truncated enzyme form is expressed in Escherichia coli BL21(DE3) cells
-