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2.4.1.132: GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase

This is an abbreviated version!
For detailed information about GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.132

Reaction

GDP-alpha-D-mannose
+
beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
=
GDP
+
alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol

Synonyms

Alg2, Alg2 mannosyltransferase, Alg2 MTase, alpha-1,3-mannosyltransferase, alpha-1,3-ManT, asparagine-linked glycosylation 2, GDP-Man:Dol-PP-GlcNAc2Man2 alpha-1,3-mannosyltransferase, GDP-Man:Man1GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-oligosaccharide-lipid mannosyltransferase II, hALG2, mannosyltransferase II, mannosyltransferase, guanosine diphosphomannose-oligosaccharide-lipid II, More, MTase, scAlg2, WfcD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.132 GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase

Engineering

Engineering on EC 2.4.1.132 - GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G393T/DELTAG1040
mutation in a patient with a congenital disorders of glycosylation designated CDG-Ii caused by ALG2 deficiency
D203A
mutation has no influence on Alg2 function
D248A
mutation has no influence on Alg2 function
E264A
mutation has no influence on Alg2 function
E335A
E335A/E343A
significant lower level of product formation, identical to that of the E335A mutant
E343A
F337A
site-directed mutagenesis, Trx-scAlg2F337A produces 26% Man3Gn2 product compared to wild-type enzyme
G337A
mutation has no influence on Alg2 function
G337E
nonfunctional enzyme variant
G337R
nonfunctional enzyme variant
G338A
mutation has no influence on Alg2 function
G377R
site-directed mutagenesis, a temperature-sensitive alg2-1 mutant containing a single missense mutation, catalytically inactive
H336A
K206A
mutation has no influence on Alg2 function
K210A
mutation has no influence on Alg2 function
K229A
mutation has no effect on growth and glycosylation
K230A
mutation causes loss of Alg2 activity
K251A
mutation has no influence on Alg2 function
N231A
mutation has no effect on growth and glycosylation
N392A
mutation has no influence on Alg2 function
P192A
mutation has no influence on Alg2 function
P359A
mutation has no influence on Alg2 function
V62G
site-directed mutagenesis, Trx-scAlg2V62G produces 25% Man3Gn2 product compared to wild-type enzyme. The HA-tagged mutant allele (3HAscAlg2V62G) fails to complement the lethality of the alg2DELTA LSY2 when grown on 5-FOA
E335A
-
site-directed mutagenesis, Trx-scAlg2E335A produces only no final product and only 32% of intermediate Man2Gn2 compared to wild-type enzyme
-
E343A
-
site-directed mutagenesis, inactive mutant
-
F337A
-
site-directed mutagenesis, Trx-scAlg2F337A produces 26% Man3Gn2 product compared to wild-type enzyme
-
H336A
-
site-directed mutagenesis, Trx-scAlg2H336A produces 8% Man3Gn2 product compared to wild-type enzyme
-
additional information