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2.4.1.13: sucrose synthase

This is an abbreviated version!
For detailed information about sucrose synthase, go to the full flat file.

Word Map on EC 2.4.1.13

Reaction

NDP-alpha-D-glucose
+
D-fructose
=
NDP
 +
sucrose

Synonyms

AcSuSy, CaSUS1, CaSUS2, CsSUS3, glucosyltransferase, uridine diphosphoglucose-fructose, GmSuSy, LjSUS3, Msus1, MtSucS1, mtSUS, PsnSuSy2, RSuS3, SBSS1, SBSS2, SH1, SS1, SS2, StSUS4, sucrose synthase, sucrose synthase 1, sucrose synthase 2, sucrose synthase 3, sucrose synthase 4, sucrose synthetase, sucrose-UDP glucosyltransferase, sucrose-uridine diphosphate glucosyltransferase, Sus, SUS-SH1, SUS1, SUS2, SUS3, sus4, SUS5, SUS6, SuSy, SuSy1, SuSy2, SuSy3, SuSy4, SuSy5, SuSy6, SuSyAc, SuSyDa, SuSyI, SuSyII, SuSyMr, SuSyNe, UDP-D-Glc:D-Fru 2-alpha-glucosyltransferase, UDP-glucose-fructose glucosyltransferase, UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase, uridine diphosphoglucose-fructose glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.13 sucrose synthase

Purification

Purification on EC 2.4.1.13 - sucrose synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isozymes: SS1 and SS2
-
2 isozymes: SS1 and SS2, partial
-
3 isozymes, heterotetramers of ss1, SS2 subunits
-
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration
-
anion exchange chromatography
at least 3 isozymes
-
gel filtration
-
His tag affinity chromatography
isoform SuSy1 and SuSy2
-
isolation of corresponding mRNA
-
native enzyme from roots partially by dialysis and ultracentrifugation
native enzyme partially by anion exchange chromatography
-
native enzyme partially by cation exchange chromatography
-
native enzyme partially by gel filtration
-
native enzyme partially by gel filtration, ammonium sulfate fractionation, and dialysis
-
Ni-NTA column chromatography
no isozymes detected by isoelectric focusing
-
partial
partial, 2 isozymes
-
recombinant
Arachnis hookeriana x Ascocenda Madame Kenny
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity
recombinant wild-type and mutant enzymes 450fold from Escherichia coli and 25fold from Saccharomyces cerevisiae by ion exchange and immobilized metal affinity chromatography, and gel filtration
recombinant wild-type and mutant enzymes from Pichia pastris by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
Strep tag affinity chromatography
Strep-Tactin column chromatography