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0.03 - 6.7
ADP-alpha-D-glucose
0.033 - 0.044
ADP-glucose
0.012
dTDP
-
pH 7.6, 30°C
0.012 - 8.3
UDP-alpha-D-glucose
0.77
UDP-GlcNAc
-
pH 8.0, 30°C
additional information
additional information
-
0.017
ADP
-
isoform SUS1, at pH 7.0 and 37°C
0.05
ADP
-
at pH 7.0 and 25°C
0.11
ADP
-
isoform SUS1, at pH 7.0 and 30°C
0.13
ADP
at pH 7.5 and 30°C
0.15
ADP
-
at pH 7.0 and 37°C
0.17
ADP
-
at pH 7.0 and 60°C
0.18
ADP
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme
0.18
ADP
Thermosynechococcus vestitus
-
at pH 7.0 and 37°C
0.21
ADP
-
at pH 7.0 and 25°C
0.3
ADP
at pH 7.0 and 60°C
0.3
ADP
-
isoform SUS4, at pH 7.0 and 37°C
0.44
ADP
-
at pH 7.0 and 60°C
0.44
ADP
-
at pH 6.0 and 37°C
1.15
ADP
-
at pH 6.5 and 30°C
1.25
ADP
-
at pH 6.5 and 30°C
1.94
ADP
-
isoform SUS2, at pH 7.0 and 30°C
33.3
ADP
-
at pH 7.5 and 37°C
0.03
ADP-alpha-D-glucose
Thermosynechococcus vestitus
-
at pH 7.0 and 37°C
0.04
ADP-alpha-D-glucose
-
at pH 8.0 and 37°C
0.17
ADP-alpha-D-glucose
-
isoform SUS1, at pH 8.0 and 30°C
0.67
ADP-alpha-D-glucose
-
isoform SUS3, at pH 8.5 and 25°C
0.78
ADP-alpha-D-glucose
-
isoform SUS2, at pH 8.0 and 30°C
1.3
ADP-alpha-D-glucose
-
at pH 7.5 and 30°C
1.6
ADP-alpha-D-glucose
at pH 7.5 and 30°C
1.8
ADP-alpha-D-glucose
-
at pH 7.3 and 26°C
3.8
ADP-alpha-D-glucose
-
at pH 8.0 and 37°C
6.7
ADP-alpha-D-glucose
-
at pH 7.5 and 37°C
0.033
ADP-glucose
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme
0.044
ADP-glucose
pH 7.0, 60°C
0.44 - 0.9
CDP
-
-
0.9
CDP
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme
1
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 37°C
1.05
D-fructose
-
25°C, isoform SuSy1
1.1
D-fructose
pH 7.5, 35°C, SuSyI
1.1
D-fructose
-
isoform SUS1, with UDP-alpha-D-glucose as cosubstrate, at pH 9.5 and 25°C
1.4
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 37°C
1.6
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 30°C
1.8
D-fructose
pH 6.5, 35°C, SuSyII
1.8
D-fructose
-
isoform SUS2, with UDP-alpha-D-glucose as cosubstrate, at pH 9.5 and 25°C
2 - 3
D-fructose
-
with ADP-alpha-D-glucose as cosubstrate, at pH 7.3 and 26°C
2.08
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 30°C
2.1
D-fructose
-
pH 8.0, 30°C
2.5
D-fructose
-
with ADP-alpha-D-glucose as cosubstrate, at pH 7.5 and 37°C
2.6
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 37°C
2.65
D-fructose
-
25°C, isoform SuSy2
2.9
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 37°C
3.7
D-fructose
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C
4.2
D-fructose
-
with ADP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C
4.37
D-fructose
-
isoform SUS1, with UDP-alpha-D-glucose as cosubstrate, at pH 9.5 and 25°C
4.8
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 8.5 and 25°C
4.97
D-fructose
-
isoform SUS4, with UDP-alpha-D-glucose as cosubstrate, at pH 9.5 and 25°C
5
D-fructose
recombinant enzyme expressed in Saccharomyces cerevisiae
5.5
D-fructose
recombinant enzyme expressed in Escherichia coli
5.5
D-fructose
-
isoform SUS1, with UDP-alpha-D-glucose as cosubstrate, at pH 9.0 and 30°C
5.6
D-fructose
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme, with ADP-glucose
5.6
D-fructose
pH 7.0, 60°C, with ADP-glucose
5.6
D-fructose
Thermosynechococcus vestitus
-
with ADP-alpha-D-glucose as cosubstrate, at pH 7.0 and 37°C
5.6
D-fructose
-
with ADP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C
6.07
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5, temperature not specified in the publication
6.49
D-fructose
-
pH 7.5, isoform SuSyC
6.49
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 7.3, temperature not specified in the publication
6.62
D-fructose
-
pH 7.5, isoform SuSyA
6.9
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C
8.4
D-fructose
-
isoform SUS1, with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 30°C
8.4
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 8.5 and 25°C
9.1
D-fructose
-
isoform SUS3, with UDP-alpha-D-glucose as cosubstrate, at pH 9.0 and 30°C
10.4
D-fructose
-
isoform SUS1, with UDP-alpha-D-glucose as cosubstrate, at pH 9.0 and 30°C
11.5
D-fructose
-
isozyme SS1
11.7
D-fructose
-
pH 7.5, isoform SuSyB
12
D-fructose
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme, with UDP-glucose
12
D-fructose
Thermosynechococcus vestitus
-
with UDP-alpha-D-glucose as cosubstrate, at pH 7.0 and 37°C
12.4
D-fructose
-
isoform SUS6, with UDP-alpha-D-glucose as cosubstrate, at pH 9.5 and 25°C
20.9
D-fructose
-
isoform SUS2, with UDP-alpha-D-glucose as cosubstrate, at pH 9.0 and 30°C
22.2
D-fructose
-
isoform SUS2, with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 30°C
40
D-fructose
-
with ADP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C
42
D-fructose
-
isoform SUS3, with UDP-alpha-D-glucose as cosubstrate, at pH 9.5 and 25°C
52
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C
120
D-fructose
pH 7.0, 60°C, with UDP-glucose
120
D-fructose
-
with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C
0.98 - 1.66
fructose
-
-
0.98 - 1.66
fructose
Leleba oldhami
-
-
0.98 - 1.66
fructose
-
cosubstrate CDP
0.98 - 1.66
fructose
-
cosubstrate ADPglucose
0.98 - 1.66
fructose
-
cosubstrate UDPglucose, ADP
0.98 - 1.66
fructose
-
cosubstrate UDP
2.08 - 3.1
fructose
-
pH 7.2
7.78
fructose
-
recombinant enzyme
40
fructose
-
cosubstrate ADPglucose
0.17 - 0.2
GDP
-
-
0.17 - 0.2
GDP
-
cosubstrate UDP, ADP, HEPES buffer
1.1
GDP
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme
0.032
sucrose
-
in the presence of 0.2 mM trehalose 6-phosphate, at pH 7.0 and 25°C
0.037
sucrose
-
in the absence of trehalose 6-phosphate, at pH 7.0 and 25°C
0.0916
sucrose
-
pH 7.6, 30°C
5.41
sucrose
-
wild type enzyme isoform SUS1, at pH 5.5 and 50°C
9.61
sucrose
-
mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C
10
sucrose
-
with UDP as cosubstrate, at pH 7.5 and 37°C
12.5
sucrose
-
with ADP as cosubstrate, at pH 7.5 and 37°C
14.28
sucrose
-
at pH 6.5 and 37 °C
16.9 - 40
sucrose
Leleba oldhami
-
-
16.9 - 40
sucrose
-
cosubstrate ADP
22.3
sucrose
-
25°C, isoform SuSy1
24.47
sucrose
-
wild type enzyme isoform SUS2, at pH 5.5 and 50°C
28.2
sucrose
-
isoform SUS1, with UDP as cosubstrate, at pH 7.0 and 30°C
30
sucrose
-
cosubstrate: UDP
30
sucrose
-
cosubstrate: UDP
30
sucrose
-
with UDP as cosubstrate, at pH 6.0 and 37°C
30
sucrose
-
with UDP as cosubstrate, at pH 7.0 and 37°C
30.3
sucrose
-
25°C, isoform SuSy2
31
sucrose
-
with UDP as cosubstrate, at pH 6.0 and 37°C
31.3
sucrose
with UDP as cosubstrate, at pH 7.5 and 30°C
31.58
sucrose
-
isoform SUS1, with UDP as cosubstrate, at pH 6.0 and 25°C
32.1
sucrose
-
isoform SUS1, with UDP as cosubstrate, at pH 7.5 and 30°C
33
sucrose
-
with UDP as cosubstrate, at pH 8.3 and 30°C
35
sucrose
-
isoform SUS4, with UDP as cosubstrate, at pH 7.0 and 37°C
35.9
sucrose
-
pH 7.0, isoform SuSyC
35.9
sucrose
-
with UDP as cosubstrate, at pH 7.0, temperature not specified in the publication
40
sucrose
-
with ADP as cosubstrate, at pH 7.0 and 60°C
40
sucrose
-
with UDP as cosubstrate, at pH 6.5 and 30°C
41.8
sucrose
-
pH 7.0, isoform SuSyA
42.7
sucrose
-
isoform SUS2, with UDP as cosubstrate, at pH 7.5 and 30°C
43.8
sucrose
-
mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C
48
sucrose
cosubstrate UDP, pH 7.0, 37°C
52 - 290
sucrose
-
cosubstrate UDP
53
sucrose
cosubstrate UDP, pH 7.0, 37°C
53
sucrose
-
isoform SUS1, with UDP as cosubstrate, at pH 7.0 and 37°C
53
sucrose
-
with UDP as cosubstrate, at pH 6.5 and 25°C
54.3
sucrose
-
isoform SUS2, with UDP as cosubstrate, at pH 7.0 and 30°C
55
sucrose
-
with UDP as cosubstrate, at pH 6.0 and 37°C
60
sucrose
recombinant enzyme expressed in Saccharomyces cerevisiae
62.5
sucrose
-
with UDP as cosubstrate, at pH 7.0 and 25°C
67.5
sucrose
-
isoform SUS3, with UDP as cosubstrate, at pH 7.5 and 30°C
80.3
sucrose
recombinant enzyme expressed in Escherichia coli
80.3
sucrose
-
isoform SUS1, with UDP as cosubstrate, at pH 7.5 and 30°C
91
sucrose
pH 6.5, 35°C, SuSyII
91
sucrose
-
isoform SUS2, with UDP as cosubstrate, at pH 6.5 and 35°C
100
sucrose
pH 6.5, 35°C, SuSyI
100
sucrose
-
isoform SUS1, with UDP as cosubstrate, at pH 6.5 and 35°C
105
sucrose
-
with ADP as cosubstrate, at pH 6.0 and 37°C
108
sucrose
-
with UDP as cosubstrate, at pH 6.5 and 30°C
109
sucrose
-
pH 7.0, isoform SuSyB
125
sucrose
-
with ADP as cosubstrate, at pH 6.0 and 37°C
140
sucrose
pH 7.0, 60°C, with ADP
145
sucrose
cosubstrate ADP, pH 7.0, 37°C
161
sucrose
-
recombinant enzyme
169
sucrose
-
with UDP as cosubstrate, at pH 7.0 and 25°C
183
sucrose
-
with ADP as cosubstrate, at pH 6.0 and 37°C
185
sucrose
cosubstrate ADP, pH 7.0, 37°C
185
sucrose
-
isoform SUS1, with ADP as cosubstrate, at pH 7.0 and 37°C
210
sucrose
-
cosubstrate: ADP
210
sucrose
-
cosubstrate: ADP
210
sucrose
-
with ADP as cosubstrate, at pH 7.0 and 37°C
220
sucrose
-
isoform SUS4, with ADP as cosubstrate, at pH 7.0 and 37°C
303
sucrose
-
with UDP as cosubstrate, at pH 6.5 and 30°C
305
sucrose
-
with ADP as cosubstrate, at pH 6.5 and 30°C
321
sucrose
pH 7.0, 60°C, with UDP
321
sucrose
-
with UDP as cosubstrate, at pH 7.0 and 60°C
0.65
TDP
-
-
1.4
TDP
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme
0.00191
UDP
-
pH 7.0
0.00191
UDP
-
pH 7.0, isoform SuSyC
0.002
UDP
-
at pH 7.0, temperature not specified in the publication
0.0041
UDP
-
pH 7.6, 30°C
0.005
UDP
at pH 7.5 and 30°C
0.0076
UDP
-
25°C, isoform SuSy1
0.02
UDP
-
at pH 6.5 and 25°C
0.03
UDP
-
isoform SUS1, at pH 7.0 and 30°C
0.032
UDP
-
25°C, isoform SuSy2
0.052
UDP
-
in the presence of 0.2 mM trehalose 6-phosphate, at pH 7.0 and 25°C
0.06
UDP
-
isoform SUS2, at pH 7.0 and 30°C
0.061
UDP
-
in the absence of trehalose 6-phosphate, at pH 7.0 and 25°C
0.061 - 0.094
UDP
-
cosubstrate TDP, pH 6
0.08
UDP
-
at pH 7.0 and 25°C
0.082
UDP
-
isoform SUS1, at pH 7.5 and 30°C
0.085
UDP
-
isoform SUS1, at pH 6.0 and 25°C
0.11
UDP
-
at pH 6.0 and 37°C
0.11
UDP
-
isoform SUS3, at pH 7.5 and 30°C
0.13
UDP
-
at pH 6.0 and 37°C
0.14
UDP
-
at pH 6.0 and 37°C
0.14
UDP
-
at pH 6.5 and 30°C
0.14 - 0.15
UDP
-
Tris buffer
0.15
UDP
-
isoform SUS2, at pH 7.5 and 30°C
0.2
UDP
recombinant enzyme expressed in Escherichia coli
0.2
UDP
-
isoform SUS1, at pH 7.5 and 30°C
0.21
UDP
-
at pH 7.0 and 25°C
0.214
UDP
-
pH 7.0, isoform SuSyB
0.22
UDP
-
at pH 7.0 and 37°C
0.22
UDP
-
isoform SUS4, at pH 7.0 and 37°C
0.3
UDP
recombinant enzyme expressed in Saccharomyces cerevisiae
0.3
UDP
-
mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C
0.31
UDP
pH 6.5, 35°C, SuSyII
0.31
UDP
-
isoform SUS2, at pH 6.5 and 35°C
0.32
UDP
-
at pH 8.3 and 30°C
0.34
UDP
pH 6.5, 35°C, SuSyI
0.34
UDP
-
isoform SUS1, at pH 6.5 and 35°C
0.39
UDP
-
isoform SUS1, at pH 7.0 and 37°C
0.41
UDP
-
at pH 6.5 and 30°C
0.59
UDP
-
mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C
0.69
UDP
-
at pH 7.0 and 60°C
0.74
UDP
-
wild type enzyme isoform SUS1, at pH 5.5 and 50°C
0.89
UDP
-
wild type enzyme isoform SUS2, at pH 5.5 and 50°C
1.07
UDP
-
pH 7.0, isoform SuSyA
1.18
UDP
-
at pH 6.5 and 37 °C
1.25
UDP
-
at pH 6.5 and 30°C
1.3
UDP
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme
1.3
UDP
Thermosynechococcus vestitus
-
at pH 7.0 and 37°C
6.6
UDP
-
at pH 7.5 and 37°C
7.8
UDP
-
at pH 7.0 and 60°C
7.8
UDP
at pH 7.0 and 60°C
0.012
UDP-alpha-D-glucose
at pH 7.5 and 30°C
0.033
UDP-alpha-D-glucose
-
isoform SUS3, at pH 8.5 and 25°C
0.044
UDP-alpha-D-glucose
-
isoform SUS1, at pH 9.5 and 25°C
0.056
UDP-alpha-D-glucose
-
isoform SUS6, at pH 9.5 and 25°C
0.058
UDP-alpha-D-glucose
-
isoform SUS2, at pH 9.0 and 30°C
0.069
UDP-alpha-D-glucose
-
isoform SUS4, at pH 9.5 and 25°C
0.07
UDP-alpha-D-glucose
-
isoform SUS2, at pH 8.0 and 30°C
0.09
UDP-alpha-D-glucose
-
at pH 8.5 and 25°C
0.09
UDP-alpha-D-glucose
-
isoform SUS3, at pH 9.0 and 30°C
0.2
UDP-alpha-D-glucose
-
isoform SUS3, at pH 9.5 and 25°C
0.21
UDP-alpha-D-glucose
-
isoform SUS1, at pH 9.0 and 30°C
0.23
UDP-alpha-D-glucose
-
at pH 7.3, temperature not specified in the publication
0.29
UDP-alpha-D-glucose
-
isoform SUS1, at pH 8.0 and 30°C
0.43
UDP-alpha-D-glucose
-
isoform SUS2, at pH 9.5 and 25°C
0.6
UDP-alpha-D-glucose
recombinant enzyme expressed in Saccharomyces cerevisiae
0.6
UDP-alpha-D-glucose
-
isoform SUS1, at pH 9.5 and 25°C
0.64
UDP-alpha-D-glucose
-
at pH 8.0 and 30°C
0.71
UDP-alpha-D-glucose
-
at pH 7.5 and 37°C
0.83
UDP-alpha-D-glucose
-
at pH 7.5, temperature not specified in the publication
0.89
UDP-alpha-D-glucose
-
at pH 8.0 and 37°C
1.14
UDP-alpha-D-glucose
-
at pH 8.0 and 30°C
1.4
UDP-alpha-D-glucose
recombinant enzyme expressed in Escherichia coli
1.7
UDP-alpha-D-glucose
Thermosynechococcus vestitus
-
at pH 7.0 and 37°C
2
UDP-alpha-D-glucose
-
at pH 7.5 and 37°C
2.7
UDP-alpha-D-glucose
-
at pH 7.5 and 37°C
2.7
UDP-alpha-D-glucose
-
at pH 7.5 and 30°C
5.3
UDP-alpha-D-glucose
-
at pH 8.0 and 37°C
6.07
UDP-alpha-D-glucose
-
isoform SUS1, at pH 9.0 and 30°C
8.3
UDP-alpha-D-glucose
-
at pH 7.5 and 37°C
0.05
UDP-glucose
-
-
0.05
UDP-glucose
-
pH 7.6, temperature not specified in the publication
0.234
UDP-glucose
-
pH 7.3
0.234
UDP-glucose
-
pH 7.5, isoform SuSyC
0.4
UDP-glucose
-
recombinant enzyme
0.42
UDP-glucose
-
25°C, isoform SuSy1
0.43
UDP-glucose
pH 6.5, 35°C, SuSyII
0.45
UDP-glucose
-
25°C, isoform SuSy2
0.5
UDP-glucose
-
pH 8.0, 30°C
0.53
UDP-glucose
-
pH 7.5, isoform SuSyB
0.6
UDP-glucose
pH 7.5, 35°C, SuSyI
0.89
UDP-glucose
pH 7.0, 60°C
1.7
UDP-glucose
Thermosynechococcus vestitus
-
pH 7.0, 37°C, recombinant enzyme
3.59
UDP-glucose
-
pH 7.5, isoform SuSyA
25
UDP-glucose
-
pH 7.6, temperature not specified in the publication
0.012 - 0.033
UDPglucose
-
-
0.012 - 0.033
UDPglucose
-
-
0.3 - 0.38
UDPglucose
-
pH 7.2
0.3 - 0.38
UDPglucose
-
isozyme SS2
0.3 - 0.38
UDPglucose
-
cosubstrate TDP
1.92 - 2.7
UDPglucose
-
-
1.92 - 2.7
UDPglucose
-
-
1.92 - 2.7
UDPglucose
Leleba oldhami
-
-
5.3 - 6
UDPglucose
-
cosubstrate fructose
5.3 - 6
UDPglucose
-
cosubstrate UDPfructose, isozyme SS2
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-
additional information
additional information
Leleba oldhami
-
kinetic study
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics, except for GDP
-
additional information
additional information
-
Michaelis-Menten kinetics, except for GDP
-