Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.123: inositol 3-alpha-galactosyltransferase

This is an abbreviated version!
For detailed information about inositol 3-alpha-galactosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.123

Reaction

UDP-alpha-D-galactose
+
myo-inositol
=
UDP
+
O-alpha-D-galactosyl-(1->3)-1D-myo-inositol

Synonyms

BhGolS1, CjGolS, CsGolS1, galactinol synthase, galactinol synthase1, galactosyltransferase, uridine diphosphogalactose-inositol, GAS, GOLS, GolS1, GS, inositol 1-alpha-galactosyltransferase, LcGolS1, LcGolS2, More, OJ1165_F02.103, Os07g0687900, OsGolS1, OsGolS2, TsGOLS2, UDP-D-galactose:inositol galactosyltransferase, UDP-galactose:myo-inositol 1-alpha-D-galactosyltransferase, uridine diphosphogalactose-inositol galactosyltransferase, XvGolS

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.123 inositol 3-alpha-galactosyltransferase

Purification

Purification on EC 2.4.1.123 - inositol 3-alpha-galactosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1591fold, copurified with a 41 and 43 kDa peptide
-
2472fold
-
partial, 41fold
-
partially from leaves, by ammonium sulfate fractionation and anion exchange chromatography
-
purification of glutathione S-transferase fusion proteins GTS-AtGolS1, 2 and 3, overexpressed in Escherichia coli
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
the soluble protein is separated by SDS-PAGE to check the molecular weight of the recombinant protein, which is then purified using Glutathione Sepharose TM 4B