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2.4.1.123: inositol 3-alpha-galactosyltransferase

This is an abbreviated version!
For detailed information about inositol 3-alpha-galactosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.123

Reaction

UDP-alpha-D-galactose
+
myo-inositol
=
UDP
+
O-alpha-D-galactosyl-(1->3)-1D-myo-inositol

Synonyms

BhGolS1, CjGolS, CsGolS1, galactinol synthase, galactinol synthase1, galactosyltransferase, uridine diphosphogalactose-inositol, GAS, GOLS, GolS1, GS, inositol 1-alpha-galactosyltransferase, LcGolS1, LcGolS2, More, OJ1165_F02.103, Os07g0687900, OsGolS1, OsGolS2, TsGOLS2, UDP-D-galactose:inositol galactosyltransferase, UDP-galactose:myo-inositol 1-alpha-D-galactosyltransferase, uridine diphosphogalactose-inositol galactosyltransferase, XvGolS

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.123 inositol 3-alpha-galactosyltransferase

Cloned

Cloned on EC 2.4.1.123 - inositol 3-alpha-galactosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
7 AtGolS genes, cloning of AtGolS1, 2 and 3, AtGolS1 and 2 are induced by drought and high-salinity stresses, but not by cold stress, AtGolS3 is induced by cold stress, but not by drought or salt stress, overexpression of glutathione S-transferase fusion proteins GTS-AtGolS1, 2 and 3 in Escherichia coli, overexpression of AtGolS2 in transgenic Arabidopsis improves drought tolerance, AtGolS3 is controlled by the transcription factor DREB1A
coding region of BhGolS1 is inframe cloned into pGEX-4T-1 downstream to GST coding region and transformed into Escherichia coli BL21(DE3) and BL21 codon plus cells, generation of transgenic tobacco plants, constructs are introduced into Agrobacterium strain LBA4404 by electroporation and transformed into tobacco via a leaf disc method
cold-inducible GolS-1 and -2 genes encode 2 distinct galactinol synthases, cloning and sequencing of the GolS-1 and -2 genes, deduced amino acid sequences, expression of GolS-1 cDNA in Escherichia coli as functional enzyme
desi and kabuli chickpea genotypes
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enzyme DNA as cloned into the predigested binary vector, tobacco is transformed by the leaf-disk method using Agrobacterium tumefaciens LBA4404 containing the CsGolS1 recombinant plasmid
expression in Escherichia coli
expression in Schizosaccharomyces pombe
gene GolS1, located on chromosome 3, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)
gene GolS2, located on chromosome 7, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)
gene MfGolS1, cloned from the cold-treated leaves by reverse transcription PCR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time quantitative PCR enzyme expression analysis, recombinant overexpression in Nicotiana tabacum cv. Zhongyan 90 seeds. Overexpression of MfGolS1 in tobacco results in elevated tolerance to freezing and chilling in transgenic plants as a result of enhanced levels of galactinol, raffinose and stachyose. Tolerance to drought and salt stresses is also increased in the transgenic tobacco plants
gene MsGolS1, sequence comparisons and phylogenetic analysis, quantitative real-time quantitative PCR enzyme expression analysis
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gene TsGOLS2, overexpression in Arabidopsis thaliana. The contents of galactinol, raffinose, and 2-oxoglutaric acid are significantly increased in transgenic plants compared to wild-type plants, and salt-stressed transgenic Arabidopsis thaliana plants exhibits higher germination rate, photosynthesis ability, and seedling growth. After being treated with osmotic stress by high concentration of sorbitol, transgenic plants retain high germination rates and grow well during early development
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identification of 20 BnGolS genes, DNA and amino acid sequence determinations and analysis, genotyping and phylogenetic analysis, genetic structure and chromosomal location, overview
identification of 9 NtGolS genes, DNA and amino acid sequence determinations and analysis, genotyping and phylogenetic analysis, genetic structure and chromosomal location, overview
LcGolS1, cDNA library construction with RNA isolated from developing lentil seeds, and screening, DNA and amino acid sequence determination and analysis, sequence comparison with isozyme LcGolS2, phylogenetic analysis, and quantitative real time PCR expression analysis
LcGolS2, cDNA library construction with RNA isolated from developing lentil seeds, and screening, DNA and amino acid sequence determination and analysis, sequence comparison with isozyme LcGolS1, phylogenetic analysis, and quantitative real time PCR expression analysis
LeGOLS-1 gene encoding a 318-amino acids peptide is cloned, gene and cDNA structure, LeGOLS-1 expression pattern in seeds and seedlings during seed maturation and germination under various conditions, hormonal control of transcription of LeGOLS-1 in the absence of gibberellin and abscisic acid, up-regulation of gene expression before maturation desiccation and again after imbibition whenever radicle protrusion is prevented