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2.4.1.120: sinapate 1-glucosyltransferase

This is an abbreviated version!
For detailed information about sinapate 1-glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.120

Reaction

UDP-alpha-D-glucose
+
sinapate
=
UDP
 +
1-O-sinapoyl-beta-D-glucose

Synonyms

BjSGT, BnSGT1, DcUSAGT1, Glucosyltransferase, uridine diphosphoglucose-sinapate, HCA-GT, More, SGT, SGT enzyme, sinapic acid:UDPG glucosyltransferase, UDP-Glc:B. napus sinapate glucosyltransferase, UDP-glucose: sinapate glucosyltransferase, UDP-glucose: sinapic acid glucosyltransferase, UDP-glucose:B. napus sinapate glucosyltransferase, UDP-glucose:sinapate glucosyltransferase, UDP-glucose:sinapate glucosyltransferase,, UDP-glucose:sinapic acid glucosyltransferase, UDPglucose:sinapic acid glucosyltransferase, UGT84A9, Uridine 5'-diphosphoglucose-hydroxycinnamic acid acylglucosyltransferase, uridine 5'-diphosphoglucose:hydroxycinnamic acid acylglucosyltransferase, USAGT

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.120 sinapate 1-glucosyltransferase

Cloned

Cloned on EC 2.4.1.120 - sinapate 1-glucosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred
construction of a cDNA library, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain M15 as soluble protein
gene DcUSAGT1, sequence comparisons, the expression levels of DcUSAGT1 gene in the purple carrot taproots may be higher than those in non-purple carrot taproots, quantitative real-time PCR expression analysis, recombinant expression of His-tagged DcUSAGT1 in Escherichia coli strain BL21(DE3)
gene SGT, recombinant expression of full-length enzyme in Brassica juncea via Agrobacterium tumefaciens mediated transformation
gene UGT84A9, semi-quantitative RT-PCR, enzyme expression analysis
genomic organization analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
heterologous expression in Escherichia coli strain BL21
stilbene synthase gene isolated from Vitis vinifera L. is cloned under control of the seed-specific napin promotor and introduced into Brassica napus L. by Agrobacterium-mediated co-transformation together with a ds-RNA-interference construct deduced from the sequence of UDP-glucose:sinapate glucosyltransferase (BnSGT1)
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