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2.4.1.109: dolichyl-phosphate-mannose-protein mannosyltransferase

This is an abbreviated version!
For detailed information about dolichyl-phosphate-mannose-protein mannosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.109

Reaction

dolichyl beta-D-mannosyl phosphate
+
L-seryl-[protein]
=
dolichyl phosphate
 +
3-O-(alpha-D-mannosyl)-L-seryl-[protein]

Synonyms

AapmtA, AfPmt1p, AnPmtA, AnPmtB, AnPmtC, dolichol phosphomannose-protein mannosyltransferase, dolichyl phosphate-mannose:protein O-mannosyltransferase, dolichyl-phosphate-mannose--protein mannosyltransferase 1, dolichyl-phosphate-mannose--protein mannosyltransferase 2, dolichyl-phosphate-mannose--protein mannosyltransferase 4, dolichyl-phosphate-mannose-protein mannosyltransferase, dolichyl-phosphate-mannose-protein mannosyltransferase 1, dolichyl-phosphate-mannose-protein mannosyltransferase 2, Drosophila O-mannosyltransferase Rotated Abdomen, Drosophila O-mannosyltransferase Twisted, HpPmt1p, mannosyltransferase, dolichol phosphomannose-protein, More, O-glycoside mannosyltransferase, O-mannosyltransferase, O-mannosyltransferase 1, ogm1, ogm2, ogm4, Oma1, Oma2, Oma4, Omg1, Omg2, Omg4, PMT, PMT1, Pmt1 O-mannosyltransferase, Pmt1A, Pmt1D, Pmt1p, Pmt1p-Pmt2p, PMT2, Pmt2A, Pmt2D, Pmt2p, PMT3, PMT4, Pmt4A, Pmt4D, Pmt4p, PMT5, Pmt5p, Pmt6, PMT7, PmtA, pmtB, pmtC, PMTI, POMT1, POMT2, protein mannosyltransferase, protein O-D-mannosyltransferase, protein O-mannosyl-transferase 1, protein O-mannosyl-transferase 2, protein O-mannosyltransferase, protein O-mannosyltransferase 1, protein O-mannosyltransferase 2, protein O-mannosyltransferase A, protein O-mannosyltransferase Pmt4, protein O-mannosyltransferases 1, protein O-mannosyltransferases 2, protein-O-mannosyltransferase-1, protein-Omannosyltransferase, RT, sPOMT2, tPOmt2, TW, Um05433, Um10749, Um11220

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.109 dolichyl-phosphate-mannose-protein mannosyltransferase

Purification

Purification on EC 2.4.1.109 - dolichyl-phosphate-mannose-protein mannosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
conidia are incubated, filtered and washed with stop buffer (pH 7.0), ground in liquid nitrogen, centrifuged, supernatant centrifuged, pellet with crude membranes resuspended, resolved by SDS-PAGE, immunodetection of target protein AN5660
gel purification of disruption mutants
glutathione Sepharose column chromatography
-
glutathione-Sepharose 4B bead chromatography
-
glutathione-Sepharose column chromatography
-
IgG-coupled magnetic bead chromatography
-
immunoaffinity chromatography, co-purification of PMT1 and PMT2 as a complex, no immuno-cross reactivity
-
isolation of microsome fraction: cells are homogenized in 10 mM Tris-HCl, pH 7.4, EDTA, sucrose, DTT, protease inhibitors, centrifugation, supernatant ultracentrifuged, precipitate used as microsomal fraction, solubilized in 20 mM Tris-HCl, pH 8.0, 2-mercaptoethanol, EDTA, n-octyl-beta-D-thioglucoside, centrifugation, supernatant used as solubilized microsome preparation, separation with SDS-PAGE and antibody staining
-
native enzyme partially
partial
samples containing both growing mycelia and aerial conidia are harvested from 1:4-diluted SDAY plates grown at 26°C for 15 days, recombinant His-tagged full-length wild-type enzyme and truncated mutant Pmt1DELTA311-902 purified from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, nickel affinity chromatography and gel filtration
solubilization with 0.5% deoxycholate and 1.2% CHAPS
-