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2.4.1.100: 2,1-fructan:2,1-fructan 1-fructosyltransferase

This is an abbreviated version!
For detailed information about 2,1-fructan:2,1-fructan 1-fructosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.100

Reaction

[beta-D-fructosyl-(2->1)-]m
+
[beta-D-fructosyl-(2->1)-]n
=
[beta-D-fructosyl-(2->1)-]m-1
 +
[beta-D-fructosyl-(2->1)-]n+1

Synonyms

1,2-beta-D-fructan:1,2-beta-D-fructan 1F-beta-D-fructosyltransferase, 1,2-beta-fructan 1F-fructosyltransferase, 1-FFT, 6G-FFT/1-FFT, FFT, fructan: fructan 1-fructosyltransferase, fructan:fructan 1-fructosyl transferase, fructan:fructan 1-fructosyltransferase, fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase, fructan:fructan fructosyl transferase, fructan:fructan-1-fructosyltransferase, fructosyltransferase, 1,2-beta-D-fructan 1F-, high DP 1-FFT, high DP fructan:fructan 1-fructosyl transferase, Wft3

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.100 2,1-fructan:2,1-fructan 1-fructosyltransferase

Engineering

Engineering on EC 2.4.1.100 - 2,1-fructan:2,1-fructan 1-fructosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N340D
the mutant shows only very low overall activity levels
N340D/S415N
the mutant shows similar properties to the W343R mutant, but the 1-nystose/neoketose ratio is decreased
N340D/W343R
the mutant shows 6G-FFT and 1-FFT activities that hold the middle between those of the N340D and W343R single mutants
N340D/W343R/S415N
the triple mutant gains the characteristic of using sucrose as a donor substrate, at the same time, this mutant can still use 1-kestose as a donor substrate, but in a much less efficient way than the wild type
S415N
the mutant shows slightly lower 6G-FFT/1-FFT activities, but the ratio between the main reaction products is not significantly affected
W343R
the mutant shows a wild type1-FFT activity but a largely reduced 6G-FFT activity
D281Y
the mutation restores the tight H-bond network and introduces typical S-type enzyme characteristics like producing similar amounts of 1-kestotriose compared to sucrose:sucrose 1-fructosyltransferases
H308T
site-directed mutagenesis
M19F
site-directed mutagenesis