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20 - 70
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after 30 min incubation, the enzyme retains about 20, 45, 75, 100, 30 and 20% activity at 20, 30, 40, 50, 60 and 70°C, respectively
35 - 50
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the enzyme is stable at temperatures (35°C and 37°C) in all incubation periods. A decrease in the enzyme activity is noticed at 40°C and 45°C after 2 h 12% and 19%, respectively. At 50°C there is about 40% loss in activity after 2 h
40 - 60
the enzyme loses about 50% of its activity after incubation for 30 min at 40°C, while the enzyme incubated at 50°C retains about 20% activity. The complete loss in catalytic activity is observed upon incubation at 60°C and higher temperatures
45
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15 min, pH 7.0, loss of activity
45 - 55
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he partially purified enzyme form retains about 76% of its original activity after 1 h at 55°C while the purified form retains about 57% after 45 min. The half-life of the partially purified form is 3286, 1150, and 718 min at 45, 50 and 55°C, respectively. The half-life of the fully purified form is 575, 319 and 176 min at 45, 50 and 55°C, respectively
55
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partially purified enzyme conjugated with different polysaccharides, 1 h. The glycosylated enzyme retains 25-57% of the original specific activity of the free enzyme dependent on the sugar conjugate type, overview
70
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20 min, complete loss of activity
35 - 55
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the enzyme displays a reasonable thermostability at 35°C and remains near 60% of initial activity when incubated at 35°C for 3 h. However, the enzyme behaves weak thermostability at a higher temperature, which exhibits the half-life of 2 h at 45°C and 1.2 h at 55°C
35 - 55
the half-life of the wild type enzyme at 35, 45 and 55°C is 4.6 h, 2.1 h and 46 min, respectively. After incubation of 0.5, 1, 2, 4 and 6 h at 35°C, the wild type enzyme retains about 86, 65, 62, 58 and 38% activity, respectively. After incubation of 0.5, 1, 2, 4 and 6 h at 45°C, the wild type enzyme retains about 78, 55, 48, 28 and 12% activity, respectively. The melting temperature is 50°C
37
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high stability for at least 24 h
40
t1/2: 16.0 min mutant enzym R433A, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
t1/2: 17.21 min mutant enzym R360S, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
t1/2: 31.4 min mutant enzym I341V, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
t1/2: 42.11 min mutant enzym R360K, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
t1/2: 47.04 min mutant enzym Y429N, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
t1/2: 52.0 min wild-type, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
t1/2: 628.0 min mutant enzym S164A, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
t1/2: 7.9 min mutant enzym H243L, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
t1/2: less than 1min mutant enzym G361F, stability is determined following the loss of activity after incubation of 1 mg/ml of protein
40
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free and immobilized enzymes, 60 min, pH 5.2, completely stable
40
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20 min, stable below
50
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t1/2: 9 min
50
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more than 1 h, completely stable
50
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although the thermal stability of the immobilised levansucrase is significantly improved in comparison to the free form, the immobilised enzyme at 50°C loses the activity faster than the free form, inactivation kinetics, overview
50
the enzyme has a half-life of 11.8 min at 50°C
50
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60 min, pH 5.2, the free enzyme loses 50% activity, the immobilized enzyme loses 40% activity
50
the enzyme has a half-life of 24.3 min at 50°C
50
the enzyme has a half-life of 290.7 min at 50°C
50
the enzyme has a half-life of 20.1 min at 50°C
50
the enzyme has a half-life of 39.4 min at 50°C
60
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60 min, pH 5.2, the free enzyme loses over 90% activity, the immobilized enzyme loses 80% activity
60
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20 min, 50% loss of activity
additional information
mutants A344P and F414W have the same behavior like the wild-type
additional information
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mutants A344P and F414W have the same behavior like the wild-type
additional information
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Ca2+ protects against thermal inactivation
additional information
Ca2+ protects against thermal inactivation