Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.10: levansucrase

This is an abbreviated version!
For detailed information about levansucrase, go to the full flat file.

Word Map on EC 2.4.1.10

Reaction

sucrose
+
[6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
=
D-glucose
+
[6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside

Synonyms

(2,6)-beta-D-fructan:D-glucose 6-fructosyltransferase, 6-SFT, 6G-FFT2, beta-2,6-fructan: D-glucose-1-fructosyltransferase, beta-2,6-fructan:D-glucose 1-fructosyltransferase, beta-2,6-fructosyltransferase, endolevanase, fructansucrase, fructosyltransferase, sucrose 6-, FTF, Lev, LEV-Y, levanase-sucrase, levansucrase, LevB1, LevB1SacB, LevJ, LEVS, LevU, Lsc, Lsc-3, Lsc2, Lsc3, LscA, LscrA, LSD, LsdA, LvnS, m1ft, M1FT protein, SacB, sucrose 6-fructosyltransferase, sucrose: 2, 6-beta-D-fructan 6-beta-Dfructosyltransferase, sucrose:2,6-beta-D-fructan:6-beta-D-fructosyltransferase, sucrose:fructan 6-fructosyltransferase, T1-LS, T2-LS, type 1 levansucrase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.10 levansucrase

Temperature Stability

Temperature Stability on EC 2.4.1.10 - levansucrase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-18 - 52
-
stable
20 - 70
-
after 30 min incubation, the enzyme retains about 20, 45, 75, 100, 30 and 20% activity at 20, 30, 40, 50, 60 and 70°C, respectively
35 - 50
-
the enzyme is stable at temperatures (35°C and 37°C) in all incubation periods. A decrease in the enzyme activity is noticed at 40°C and 45°C after 2 h 12% and 19%, respectively. At 50°C there is about 40% loss in activity after 2 h
35 - 55
40 - 60
the enzyme loses about 50% of its activity after incubation for 30 min at 40°C, while the enzyme incubated at 50°C retains about 20% activity. The complete loss in catalytic activity is observed upon incubation at 60°C and higher temperatures
45
-
15 min, pH 7.0, loss of activity
45 - 55
-
he partially purified enzyme form retains about 76% of its original activity after 1 h at 55°C while the purified form retains about 57% after 45 min. The half-life of the partially purified form is 3286, 1150, and 718 min at 45, 50 and 55°C, respectively. The half-life of the fully purified form is 575, 319 and 176 min at 45, 50 and 55°C, respectively
55
-
partially purified enzyme conjugated with different polysaccharides, 1 h. The glycosylated enzyme retains 25-57% of the original specific activity of the free enzyme dependent on the sugar conjugate type, overview
70
-
20 min, complete loss of activity
additional information