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2.4.1.10: levansucrase

This is an abbreviated version!
For detailed information about levansucrase, go to the full flat file.

Word Map on EC 2.4.1.10

Reaction

sucrose
+
[6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
=
D-glucose
 +
[6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside

Synonyms

(2,6)-beta-D-fructan:D-glucose 6-fructosyltransferase, 6-SFT, 6G-FFT2, beta-2,6-fructan: D-glucose-1-fructosyltransferase, beta-2,6-fructan:D-glucose 1-fructosyltransferase, beta-2,6-fructosyltransferase, endolevanase, fructansucrase, fructosyltransferase, sucrose 6-, FTF, Lev, LEV-Y, levanase-sucrase, levansucrase, LevB1, LevB1SacB, LevJ, LEVS, LevU, Lsc, Lsc-3, Lsc2, Lsc3, LscA, LscrA, LSD, LsdA, LvnS, m1ft, M1FT protein, SacB, sucrose 6-fructosyltransferase, sucrose: 2, 6-beta-D-fructan 6-beta-Dfructosyltransferase, sucrose:2,6-beta-D-fructan:6-beta-D-fructosyltransferase, sucrose:fructan 6-fructosyltransferase, T1-LS, T2-LS, type 1 levansucrase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.10 levansucrase

Crystallization

Crystallization on EC 2.4.1.10 - levansucrase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 different crystal types I, II and III, 3 methods for crystallization of the protein from NaH2PO4, 0.2 M, pH 4.3, structure analysis, overview
-
crystal structures of the inactive single site mutants D86A, D247A and E342A of Bacillus subtilis levansucrase and from the sucrose- and raffinose-bound complexes of the E342A mutant, minimal structural consequences of the D247A, D86A mutations
crystallization by precipitation with ethanol and ammonium sulfate and dialysis against distilled water or from 20 mM phosphate buffer, pH 5.0, + 0.5 mM ammonium sulfate and 5% v/v 2-methyl-2,4-pentanediol, multiple isomorphous replacements with 3 heavy atom derivatives for x-ray tertiary structure analysis
-
mutant enzyme S164A, 12 days of micro-dialysis of the purified protein (8 g/l) against deionized water, PDB accession code 2VDT
hanging drop vapour diffusion method, mixing of 0.001 ml of 25 mg/ml protein solution containing 25 mM Tris-HCl, pH 7.5, 150 mM NaCl, with 0.001 ml of crystallisation reagent containing 35% PEG 2000 MME, 0.1 M KSCN, X-ray diffraction structure determination and analysis at 2.77 A resolution, molecular replacement using Gluconacetobacter diazotrophicus levansucrase LsdA, PDB ID 1W18, as a search model, enzyme structure comparisons, overview
hanging drop vapor diffusion method, using 28% (v/v) glycerol, 14% (w/v) PEG4000, 2.5 mM manganese(II) chloride tetrahydrate, 2.5 mM cobalt(II) chloride hexahydrate, 2.5 mM nickel(II) chloride hexahydrate, 2.5 mM zinc acetate dihydrate
in complex with levanbiose, hanging drop vapor diffusion method, using 28% (v/v) glycerol, 14% (w/v) PEG4000, 2.5 mM manganese(II) chloride tetrahydrate, 2.5 mM cobalt(II) chloride hexahydrate, 2.5 mM nickel(II) chloride hexahydrate, and 2.5 mM zinc acetate dihydrate
catalytic center of levansucrase LsdA, PDB ID 1W18, with raffinose molecule, from PDB ID 3BYN, in the substrate-binding pocket
the three-dimensional structure of LsdA determined by X-ray crystallography at a resolution of 2.5 A
catalytic center of levansucrase LsdA, PDB ID 1W18, with raffinose molecule, from PDB ID 3BYN, in the substrate-binding pocket
-
crystallization from saturated (NH4)2SO4-solution, pH 6.0, 4°C, 20 mM potassium phosphate, 0.2 M NaCl
-