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2.4.1.1: glycogen phosphorylase

This is an abbreviated version!
For detailed information about glycogen phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.1

Reaction

[(1->4)-alpha-D-glucosyl]n
+
phosphate
=
[(1->4)-alpha-D-glucosyl]n-1
 +
alpha-D-glucose 1-phosphate

Synonyms

1,4-alpha-glucan phosphorylase, alpha-1,4 glucan phosphorylase, alpha-1,4-glycan phosphorylase, alpha-glucan phosphorylase, alpha-glucan phosphorylase H, alpha-glucan/maltodextrin phosphorylase, alphaGP, amylopectin phosphorylase, amylophosphorylase, CcStP, cyosolic phosphorylase, GlgP, glucan phosphorylase, glucosan phosphorylase, glycogen phosphorylase, glycogen phosphorylase a, glycogen phosphorylase b, glycogen phosphorylase-a, GP, GP b, GPA, GPase, GPase a, GPb, GPBB, GPH, granulose phosphorylase, MalP, maltodextrin phosphorylase, More, muscle glycogen phosphorylase, muscle phosphorylase, muscle phosphorylase a and b, myophosphorylase, PF1535, Phb, PHO, Pho 2, Pho1, phosphorylase a, phosphorylase b, phosphorylase, alpha-glucan, plastidial phosphorylase, polyphosphorylase, potato phosphorylase, RMGPa, rmGPb, SP, starch phosphorylase, starch phosphorylase H, stGP, StP, tGPGG, type L alpha-glucan phosphorylase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.1 glycogen phosphorylase

Purification

Purification on EC 2.4.1.1 - glycogen phosphorylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzymes, DC1 and 2, starch adsorption, DEAE-Sephadex
-
2 isozymes, anion exchange, gel filtration, amylopectin-Sepharose affinity column
-
2 isozymes: I and II
-
2 isozymes: termed slow and fast, heat treatment, ammonium sulfate, DEAE-cellulose, preparative gel electrophoresis
-
2 of 3 isozymes: cytoplasmic I and major chloroplastic III form
-
alumina C-gamma gel, calcium phosphate gel, protamine sulfate, acetone, ethanol, ammonium sulfate, DEAE-cellulose, hydroxyapatite
-
ammonium sulfate, affinity chromatography, ion-exchange, gel filtration
-
ammonium sulfate, DEAE-cellulose, hydroxylapatite
-
ammonium sulfate, DEAE-cellulose, partial purification
-
ammonium sulfate, DEAE-cellulose, Sephadex G-200
-
ammonium sulfate, DEAE-cellulose, Sephadex G100, Sephadex g-200, starch-Sepharose affinity chromatography
-
ammonium sulfate, DEAE-Sephacel, Sepharose CL-6B
-
ammonium sulfate, DEAE-Sephadex, Sephadex G-100, Sepharose 6B
-
ammonium sulfate, polyethylenglycol, DEAE-cellulose, CM-Sephadex
-
ammonium sulfate, Q-Sepharose, Mono Q, gel filtration
-
ammonium sulfate, Sephacryl S-200, DEAE-cellulose
-
cytoplasmic phosphorylase: ammonium sulfate, DEAE-cellulose, glycogen-Sepharose, chloroplast phosphorylase: DEAE-cellulose, Biogel A, partial purification
-
cytosolic isozyme from leaves 7140fold to homogeneity by affinity chromatography
DEAE-cellulose, BioGel P-150, partial purification
-
from muscle, to homogeneity by several chromatographic steps and preparative SDS-PAGE
from seedlings, 299fold to homogeneity by protamine sulfate and ammonium sulfate fractionation, anion exchange chromatography, and dextrin affinity chromatography
-
from skeletal muscle
-
heart isozymes I and II, ammonium sulfate, DEAE-Sephacel, AMP-Sepharose, muscle enzyme, ammonium sulfate, DEAE-Sephacel
-
isozymes a, ab and b, acid treatment, DEAE-Sephacel, 5'-AMP-Sepharose
-
isozymes a, streptomycin sulfate, ammonium sulfate, 50°C, DE52-cellulose, aminobutyl-agarose, Sephacryl S-300, isoenzyme b, DE52-cellulose, 5'-AMP-Sepharose
-
isozymes I and II
Voandzeia subterranea
-
isozymes P-1 and P-2, ammonium sulfate, DE-52, amylose-Sepharose, BioGel
-
native enzyme from skeletal muscle
native glycogen phosphorylase b from skeletal muscle, glycogen phosphorylase a is prepared from glycogen phosphorylase b by phosphorylation
non-chloroplastic phosphorylase I, ammonium sulfate, dextrin-Sepharose
-
partial
partial purification
-
pH 5.3, protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-200
-
pH 5.4, ammonium sulfate, DEAE-cellulose, 5'-AMP-Sepharose
-
phenyl-Sepharose, maltodextrin-Sepharose 6B, methyl-column
-
phosphorylase ab hybrid
-
phosphorylase I, starch-adsorption, heat treatment, DEAE-cellulose, Sephadex G-200
-
phosphorylase I, starch-Sepharose column, DEAE-cellulose, phosphorylase II, DEAE-cellulose, starch-Sepharose
-
phosphorylase II
-
phosphorylases A, B and C
-
phosphorylases b, ab and a, DEAE-Sephacel, AMP-Sepharose
-
polyethylene glycol 6000, ammonium sulfate, gel filtration
-
protamine sulfate, ammonium sulfate, DEAE-Sephadex, Sephadex G-200
-
recombinant C-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography, recombinant cellulose-binding module–intein-alphaGP fusion protein by affinity adsorption on amorphous cellulose followed by intein self-cleavage
-
recombinant His-tagged enzyme from Escherichia coli 6.3fold to homogeneity by heat treatment at 70°C and nickel affinity chromatography
recombinant protein expressed in Escherichia coli
-
recombinant wild-type and mutant enzymes from Escherichia coli
-
starch-Sepharose column
Gracilaria sordida
-
starch-Sepharose, Sephacryl S-300
Gracilaria sordida
-
Streptomycin sulfate, ammonium sulfate, heat, DEAE-cellulose, Sepharose 2B, preparative gel electrophoresis
-
Streptomycin, 33% ethanol, DEAE-Sephadex, glycogen-Sepharose
-
Streptomycin, ammonium sulfate, DEAE-cellulose, aminobutyl-Sepharose, hydroxyapatite
-
Streptomycin, ammonium sulfate, DEAE-Sephadex, Sephadex G-200
-
stromal phosphorylase, ammonium sulfate, Sephacryl 300 Hr, starch adsorption, Q-Sepharose, Mono Q
-
wild-type and mutant enzymes
-
Wild-type and the H334G mutant enzyme produced in Escherichia coli