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2.4.1.1: glycogen phosphorylase

This is an abbreviated version!
For detailed information about glycogen phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.1

Reaction

[(1->4)-alpha-D-glucosyl]n
+
phosphate
=
[(1->4)-alpha-D-glucosyl]n-1
 +
alpha-D-glucose 1-phosphate

Synonyms

1,4-alpha-glucan phosphorylase, alpha-1,4 glucan phosphorylase, alpha-1,4-glycan phosphorylase, alpha-glucan phosphorylase, alpha-glucan phosphorylase H, alpha-glucan/maltodextrin phosphorylase, alphaGP, amylopectin phosphorylase, amylophosphorylase, CcStP, cyosolic phosphorylase, GlgP, glucan phosphorylase, glucosan phosphorylase, glycogen phosphorylase, glycogen phosphorylase a, glycogen phosphorylase b, glycogen phosphorylase-a, GP, GP b, GPA, GPase, GPase a, GPb, GPBB, GPH, granulose phosphorylase, MalP, maltodextrin phosphorylase, More, muscle glycogen phosphorylase, muscle phosphorylase, muscle phosphorylase a and b, myophosphorylase, PF1535, Phb, PHO, Pho 2, Pho1, phosphorylase a, phosphorylase b, phosphorylase, alpha-glucan, plastidial phosphorylase, polyphosphorylase, potato phosphorylase, RMGPa, rmGPb, SP, starch phosphorylase, starch phosphorylase H, stGP, StP, tGPGG, type L alpha-glucan phosphorylase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.1 glycogen phosphorylase

General Stability

General Stability on EC 2.4.1.1 - glycogen phosphorylase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
5'-AMP stabilizes dilute enzyme solutions in media of high ionic strength, e.g. 50 mM ammonium sulfate or MgCl2
-
dialysis for 24 h against water, stable to, phosphorylase A or C
-
dithiothreitol stabilizes
-
freeze-thawing inactivates
freezing inactivates completely
-
glycerol, 10%, stabilizes during purification
-
glycogen, AMP or caffeine prevent proteolytic action of trypsin
-
half-lives of wild-type and mutant enzymes under dissociation/inactivation conditions at 30°C and pH 7.0 in 0.4 M imidazole citrate buffer, pH 7.5, and 0.1 mM L-cysteine, overview
-
increase in thermostability and reusability of the enzyme immobilzed on brick dust
-
phosphate protects against thermal inactivation
-
Streptomycin sulfate or ammonium sulfate precipitation inactivates b-, not a-isozyme
-
sucrose, 10%, stabilizes during purification
-
unstable in the absence of phosphate, half-life at 4°C: less than 12 h