2.3.3.9: malate synthase

This is an abbreviated version!
For detailed information about malate synthase, go to the full flat file.

Word Map on EC 2.3.3.9

2.3.3.9

isocitrate

peroxisomal

icl

glyoxysomal

catalase

citrate

tricarboxylic

tuberculosis

shunt

cotyledons

seedling

microbodies

cucumber

gluconeogenesis

beta-oxidation

4.1.3.1

anaplerotic

fumarase

thiolase

cucumis

phosphotransacetylase

trosy

hydroxypyruvate

acetate-grown

agriculture

3-ketoacyl-coa

pumpkin

Reaction

Synonyms

EC 4.1.3.2, GCE, GCE(13-573), glcB, glyoxylate cycle enzyme, glyoxylate transacetase, glyoxylic transacetase, L-malate glyoxylate-lyase (CoA-acetylating), malate synthase, malate synthase 1, malate synthase A, malate synthase G, malate synthase H, malate synthetase, malic synthetase, malic-condensing enzyme, MLS, MSA, MSG, MSH, SSO1334

ECTree

2 Transferases

2.3 Acyltransferases

2.3.3 Acyl groups converted into alkyl groups on transfer

2.3.3.9 malate synthase

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Results	in table
2	Activating Compound
26198	AA Sequence
1	Application
1	CAS Registry Number
25	Cloned(Commentary)
6	Crystallization (Commentary)
10	Expression
4	General Information
10	General Stability
7	IC50 Value
99	Inhibitors
43	Ki Value [mM]
71	KM Value [mM]
20	Localization
51	Metals/Ions
78	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
110	Organism
13	Pathway
70	PDB ID
22	pH Optimum
12	pH Range
9	pH Stability
1	pI Value
5	Posttranslational Modification
20	Protein Variants
45	Purification (Commentary)
7	Reaction
3	Reaction Type
105	Reference
1	Renatured (Commentary)
37	Source Tissue
37	Specific Activity [micromol/min/mg]
7	Storage Stability
109	Substrates and Products (Substrate)
61	Subunits
66	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
8	Temperature Range [°C]
18	Temperature Stability [°C]
13	Turnover Number [1/s]

Purification

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Purification on EC 2.3.3.9 - malate synthase

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25 entries

DEAE Sepharose column chromatography, HisTrap HP column chromatography, and Superdex 200 pg gel filtration

Euglena gracilis

715749

glutathione affinity chromatography, proteolytic removal of the GST tag, anion exchange chromatography

Pseudomonas aeruginosa

702873

glutathione Sepharose column chromatography

Paracoccidioides sp.

735853

HiTrap column chromatography

Escherichia coli

735671

Ni-chelate affinity chromatography, His-, Trx-, and S-tag removed by cleavage with enterokinase

Paracoccidioides brasiliensis

Q5YLB8

702909

Ni-NTA agarose column chromatography and Superdex 200 gel filtration

3 entries

Ni-NTA column chromatography, and gel filtration

Paracoccidioides sp.

735447

recombinant enzyme

Mycobacterium tuberculosis

638507

recombinant enzymes

2 entries

reverse phase chromatography, anion-exchange column chromatography, and gel filtration

Haloferax volcanii

D4GTL2

719191