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2.3.3.5: 2-methylcitrate synthase

This is an abbreviated version!
For detailed information about 2-methylcitrate synthase, go to the full flat file.

Word Map on EC 2.3.3.5

Reaction

propanoyl-CoA
+
H2O
+
oxaloacetate
=
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
+
CoA

Synonyms

2-MC synthase, 2-MCS, 2-MCS1, 2-methylcitrate oxaloacetate-lyase, 2-methylcitrate synthase 2, citrate synthase II, citrate synthase/2-methylcitrate synthase, EC 4.1.3.31, MCA condensing enzyme, MCS, Mcs1, mcsA, Methylcitrate synthase, methylcitrate synthetase, mmgD, MscA, PrpC, prpC2, Rv1131

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.5 2-methylcitrate synthase

Cloned

Cloned on EC 2.3.3.5 - 2-methylcitrate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli with polyhistidine tags
gene mscA, DNA and amino acid sequence determination and analysis, overexpression in Aspergillus nidulans
-
gene prpC sequenced
gene sequenced
-
genome sequencing, prpDBC gene cluster, prpC2 gene for 2-methylcitrate synthase, cloned into vector pTrc99a and expressed in Escherichia coli
into the pCR2.1 and pUChph1 vectors
-
into the vector pJET1.2 for sequencing, into a modified pET43.1a vector for expression in Escherichia coli BL21DE3 Rosetta 2 cells
pRES1, prpC gene cloned into mutant Escherichia coli W620
-
prpC cloned, sequenced and expressed in Escherichia coli
-
prpC encoded enzyme complements a prpC mutant of Salmonella enterica serovar Typhimurium, transduction of genomic DNA ligated into the cosmid pHC79 to Escherichia coli S17-1
-
the chromosomal gene encoding the 2-methyl-cis-aconitate hydratase is disrupted by direct insertion of a copy of an additional 2-methylcitrate synthase gene (prpc). 2-Methylcitrate synthase is expressed under the control of the constitutive kanamycin-resistance gene resulting in up to 20fold higher specific 2-methylcitrate synthase activities in comparison to the wild-type. The disruption of the acnM gene by insertion of prpC leads to a propionate- and levulinate-negative phenotype of the engineered strains and accumulation of 2-methylcitrate in the medium
-