2.3.3.16: citrate synthase (unknown stereospecificity) This is an abbreviated version! For detailed information about citrate synthase (unknown stereospecificity), go to the full flat file .
Reaction
acetyl-CoA +
H2O +
oxaloacetate =
citrate +
CoA
Synonyms bifunctional citrate synthase/2-methylcitrate synthase, CCNA_01983, CIT1, CitA, citrate condensing enzyme, citrate oxaloacetate-lyase (CoA-acetylating), citrate oxaloacetate-lyase, CoA-acetylating, citrate synthase, citrate synthase Cit1, citrate synthase/2-methylcitrate synthase, citrate synthetase, citric synthase, citric-condensing enzyme, citrogenase, CitZ, CS, CS1, CS2, CS3, CS4, CSI, CSY, CSY4, CTS, EC 4.1.3.7, gltA, GltA2, MCS, mitochondrial citrate synthase, mmgD, More, Msed_1522, oxalacetic transacetase, oxaloacetate transacetase, peroxisomal citrate synthase, Rv0896, SbnG, Si-citrate synthase, sll0401, SSO2589, St0589, St1805, synthase, citrate, TTHA1343, type II citrate synthase
ECTree
Temperature Stability
Temperature Stability on EC 2.3.3.16 - citrate synthase (unknown stereospecificity)
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101
-
recombinant enzyme, stable
40 - 55
the enzyme maintains almost all of its initial activity after incubation for 20 min at 40°C or below. The half-life of the enzyme is 10 min at 50°C and is increased to 210 min in the presence of oxaloacetate. Enzyme activity is undetectable after incubation for 10 min at 55°C
55
half-life about 35 min
100
-
half-life: 20 min
100
half-life: 17 min, structural basis
50
-
rapid inactivation
50
-
in presence of oxaloacetate 70% of activity retained after 10 min, in absence of oxaloacetate complete loss of activity, acetyl-CoA does not protect
50
20 min, 11% loss of activity
80
-
90% of activity after 10 min
80
-
t1/2 of recombinant wild-type is 10 min, t1/2 of recombinant mutant G196V is 8 min, inactivation of the mutant occurs in 2 stages: a first slow and a second rapid one
80
-
65% of activity retained after 10 min
90
-
hexameric enzyme form, half-life: 18 min, dimeric enzyme form, half-life: 12 min
90
-
mutant G196V: t1/2: below 1 min, t1/2 wild-type: 5 min
95
-
90% loss of activity after 10 min
95
5 min, about 2% loss of activity
additional information
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thermal stability of mutants
additional information
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cold-lability in presence of 3 M KCl, but not with NaCl up to 5 M
additional information
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oxaloacetate protects against heat denaturation
additional information
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-
additional information
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thermostability of wild-type and chimeric mutants
additional information
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thermostability of wild-type and chimeric mutants
additional information
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effect of C43 mutation